LOCUS Exported 11403 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION GAL1 driven Cas9. gRNA can be cloned into BplI sites.. ACCESSION . VERSION . KEYWORDS bRA77 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11403) AUTHORS Anand R, Beach A, Li K, Haber J TITLE Rad51-mediated double-strand break repair and mismatch correction of divergent substrates. JOURNAL Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12. PUBMED 28405019 REFERENCE 2 (bases 1 to 11403) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11403 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" CDS complement(783..1877) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(1878..2285) /gene="S. cerevisiae LEU2" /label=LEU2 promoter promoter 2439..2880 /gene="S. cerevisiae GAL1" /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" protein_bind 2439..2556 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" primer_bind 2851..2874 /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" CDS 2900..7003 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7016..7036 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7040..7060 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7064..7084 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7085..7150 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" terminator 7169..7416 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(7185..7203) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" rep_origin complement(7476..7931) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7613..7634) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 7825..7844 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8057..8079 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 8071..8088 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 8072..8088 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 8095..8114 /label=T7 /note="T7 promoter, forward primer" promoter 8095..8113 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 8146..8162 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 8146..8162 /label=pBluescriptSK /note="For pBluescript vector" promoter 8194..8462 /label=SNR52 promoter /note="promoter for the S. cerevisiae small nucleolar RNA gene SNR52" misc_RNA 8490..8564 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" terminator 8569..8588 /label=SUP4 terminator /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" promoter complement(8630..8648) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8669..8685) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8669..8685) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8682..8704) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8693..8709 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8717..8747) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8762..8783 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8900..8917) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9071..9659) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9151..9170) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9830..10690) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10453..10472 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10691..10795) /gene="bla" /label=AmpR promoter misc_feature 10832..11335 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" primer_bind 11376..11394 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcgaattcg 241 agctcgttta aactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca 301 ttcacatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 361 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 421 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatataaa 481 ttaggaatca tagtttcatg attttctgtt acacctaact ttttgtgtgg tgccctcctc 541 cttgtcaata ttaatgttaa agtgcaattc tttttcctta tcacgttgag ccattagtat 601 caatttgctt acctgtattc ctttacatcc tcctttttct ccttcttgat aaatgtatgt 661 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 721 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 781 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 841 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 901 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 961 aagatagtgg cgatagggtt gaccttattc tttggcaaat ctggagcaga accgtggcat 1021 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 1081 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1141 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1201 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1261 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1321 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1381 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1441 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1501 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1561 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1621 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc agccttcttg 1681 gaggcttcca gcgcctcatc tggaagtgga acacctgtag catcgatagc agcaccacca 1741 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1801 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1861 ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata tattgctgaa 1921 atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac 1981 aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat ttagtcatga 2041 acgcttctct attctatatg aaaagccggt tccggcgctc tcacctttcc tttttctccc 2101 aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc 2161 cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa 2221 aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg agtattccca 2281 cagttgtatt ctgggcctcc atgtcgctgg ccgggtgacc cggcggggac gaggcaagct 2341 aaacagatct gtaaagagcc ccattatctt agcctaaaaa aaccttctct ttggaacttt 2401 cagtaatacg cttaactgct cattgctata ttgaagtacg gattagaagc cgccgagcgg 2461 gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc 2521 ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta 2581 gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa ccttcaaatg 2641 aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc cttatttctg 2701 gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata aatgcaaaaa 2761 ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact tcttattcaa 2821 atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa cgtcaaggag 2881 aaaaaacccg gatctcaaaa tggataaaaa gtatagtatt ggtttagata ttggtactaa 2941 ctctgtgggt tgggcagtta tcactgacga atataaagtt ccatcaaaga aatttaaggt 3001 gttaggtaac actgacagac actcaataaa aaagaatctt atcggtgctc ttttgttcga 3061 ctccggtgaa actgccgagg ctacacgttt aaaaagaaca gcaagaagaa gatatacccg 3121 tagaaaaaat agaatatgtt atttacaaga aatcttttct aatgaaatgg ctaaagttga 3181 tgattccttt ttccatagat tggaagagtc atttttggtt gaagaagaca aaaagcatga 3241 gagacatcca atctttggga atatagttga tgaagtggct taccatgaaa aatatcctac 3301 catttatcat ttaagaaaga aattggtaga ttcaactgat aaagctgacc ttagattaat 3361 ctatttagca cttgcccata tgattaaatt tagaggtcat tttttgattg aaggtgattt 3421 gaacccagat aattctgacg tggataaatt atttattcaa ttagtccaaa cctacaacca 3481 attatttgag gaaaatccaa ttaatgctag tggtgtcgat gccaaagcta tattatcagc 3541 cagattatca aaatctagac gtttggaaaa tttgattgcc caattgccag gagaaaaaaa 3601 gaatggatta tttggaaact tgatcgcatt atcattgggt ttgacaccaa attttaaatc 3661 taattttgat ttagctgaag atgctaaatt acaattatca aaagacacct atgacgacga 3721 tttggacaat ttacttgctc aaattggtga tcaatatgca gatttgttct tagctgctaa 3781 aaacttatct gatgctattt tgttgtctga tattttgaga gtgaacacag aaataaccaa 3841 agctccatta tcagcatcta tgatcaaacg ttatgatgaa caccatcagg atttgacttt 3901 attgaaagct ttggtgagac aacaattgcc agagaagtat aaagaaatct ttttcgatca 3961 atctaaaaac gggtatgcag gttatattga tgggggtgcc tcccaagagg aattttacaa 4021 atttataaaa cctattttag aaaagatgga tgggactgag gaacttttgg tcaaattgaa 4081 cagagaagat ttgttacgta aacagagaac ttttgataat ggtagtatac ctcaccaaat 4141 tcatttgggt gagttgcatg caattttaag aagacaagaa gatttttatc catttttaaa 4201 agataataga gaaaaaatcg agaaaatttt aacctttaga attccatact atgttgggcc 4261 tttggctaga ggtaattcaa gatttgcctg gatgacacgt aaatcagaag aaactattac 4321 cccttggaat tttgaagagg ttgttgataa aggagcatca gcacagagtt ttattgaaag 4381 aatgaccaat ttcgataaaa acttaccaaa tgaaaaagtt ttaccaaaac attccttgtt 4441 atacgaatat tttactgttt acaatgaact tacaaaggtt aaatatgtta ctgaaggtat 4501 gcgtaagcca gcctttttat ctggagaaca gaaaaaggca atagttgatt tattgtttaa 4561 aacaaataga aaagttactg ttaaacaatt aaaagaagat tactttaaga aaattgaatg 4621 ttttgattca gttgaaatca gtggtgttga agacagattt aatgctagtt taggaactta 4681 ccatgattta cttaaaatta tcaaagataa agatttcttg gataacgaag aaaatgaaga 4741 cattttagaa gacattgttt taaccttaac tttattcgaa gatagagaga tgattgaaga 4801 acgtttgaag acttatgcac atttgtttga cgataaagtg atgaaacagt tgaaaagaag 4861 acgttatact ggatggggta gattgtctcg taaattgatc aatggaatta gagataaaca 4921 aagtggtaaa actatcttgg actttttgaa atctgacgga tttgctaata gaaatttcat 4981 gcaattgatc cacgacgata gtttgacatt taaagaagac atccaaaagg cccaagtgag 5041 tgggcaaggt gattcattac atgaacatat tgcaaattta gccggatctc ctgctattaa 5101 gaaagggata ttacaaactg ttaaagttgt ggatgaatta gtgaaagtaa tgggaagaca 5161 taaacctgaa aacattgtca ttgagatggc aagagaaaat caaactacac aaaaaggaca 5221 gaaaaatagt agagaacgta tgaaaagaat agaagagggt attaaagaat tgggtagtca 5281 aatattgaaa gaacacccag tggaaaatac ccagttgcaa aatgaaaaat tatatcttta 5341 ctaccttcaa aatggacgtg atatgtatgt tgatcaggaa ttagatataa atagactttc 5401 agattatgat gtagatcata tagttccaca atctttcttg aaagatgatt ccatagacaa 5461 taaagtatta actagaagtg ataaaaatag aggtaaaagt gataatgtcc caagtgagga 5521 agtcgtcaaa aagatgaaaa attactggcg tcaacttttg aatgctaaat taattactca 5581 aagaaaattt gataatttga ctaaagcaga aagaggtggg ctttctgaat tagataaagc 5641 cgggttcatt aaaagacaat tggtcgaaac tagacaaatt actaaacatg ttgcccaaat 5701 tttagattcc cgtatgaaca ctaagtatga cgaaaatgat aagttaatac gtgaggttaa 5761 agtcattact ttaaaatcaa aacttgtctc tgatttcaga aaggatttcc aattctataa 5821 agttagagaa attaataatt atcatcatgc tcatgatgca tatttgaatg ctgtagttgg 5881 aactgcttta atcaagaaat accctaaatt agaatctgaa tttgtatatg gtgattacaa 5941 agtctatgat gttagaaaga tgattgctaa atcagaacaa gaaattggta aagctacagc 6001 taaatacttc ttttactcta acattatgaa tttctttaaa acagaaatta ctttggcaaa 6061 cggtgaaatt agaaaaagac ctcttattga aacaaatggt gagactggag agatagtttg 6121 ggacaaaggg cgtgatttcg ctactgttag aaaagtttta tcaatgccac aagttaacat 6181 tgtaaagaaa acagaggttc aaactggtgg tttctcaaaa gaaagtattt tgcctaaaag 6241 aaatagtgat aaattgattg ccagaaaaaa ggattgggat ccaaagaaat atggtggttt 6301 cgactcacca accgtagcct attctgtttt ggttgtggca aaggttgaaa agggtaaaag 6361 taaaaagctt aaatcagtaa aagaactttt gggtattaca ataatggaaa gaagttcctt 6421 tgaaaagaac cctattgatt ttttggaagc taaaggttat aaggaagtaa agaaggactt 6481 aataatcaaa ttgcctaaat attctttatt tgaattagaa aatgggagaa aaagaatgtt 6541 ggcttctgct ggagaattgc aaaagggtaa tgaattagca ttgccttcca aatatgttaa 6601 cttcttgtat ttagcttcac actatgaaaa gttgaaaggg tcaccagaag ataacgagca 6661 aaaacaatta tttgttgaac aacacaaaca ctacttagat gagattatag aacaaattag 6721 tgaattcagt aaaagagtga tattagctga tgcaaattta gataaagttt tgtcagccta 6781 taacaaacat agagataagc caattagaga acaagcagaa aacattattc acttatttac 6841 ccttaccaat ttaggagcac ctgctgcttt caagtatttt gatacaacaa ttgatcgtaa 6901 aagatatacc tcaacaaaag aagtcttaga cgccacctta attcatcaat caatcactgg 6961 attgtatgag acaagaattg atttgtctca attgggtggt gatgaagggg ctgatcctaa 7021 gaagaaaaga aaagttgatc caaagaaaaa gcgtaaggtg gatcctaaga aaaagagaaa 7081 ggttgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga 7141 cgatgacaag tgataatctc ttctcgagtc atgtaattag ttatgtcacg cttacattca 7201 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 7261 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 7321 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 7381 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtactgcgg tgtgaaatac 7441 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt 7501 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 7561 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 7621 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 7681 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 7741 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 7801 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 7861 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 7921 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 7981 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 8041 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac 8101 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga 8161 tcccccgggc tgcaggaatt cgatatcaag ctttctttga aaagataatg tatgattatg 8221 ctttcactca tatttataca gaaacttgat gttttctttc gagtatatac aaggtgatta 8281 catgtacgtt tgaagtacaa ctctagattt tgtagtgccc tcttgggcta gcggtaaagg 8341 tgcgcatttt ttcacaccct acaatgttct gttcaaaaga ttttggtcaa acgctgtaga 8401 agtgaaagtt ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa agataaatga 8461 tcctagatgc gagatatcct catgcctagg ttttagagct agaaatagca agttaaaata 8521 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgctt tttttgtttt 8581 ttatgtctac cgtcgacctc gagggggggc ccggtaccag cttttgttcc ctttagtgag 8641 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 8701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 8761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 8821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 8881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 8941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 9001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 9061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 9121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 9181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 9241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 9301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 9361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 9421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 9481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 9541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 9601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 9661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 9721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 9781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 9841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 9901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 9961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 10021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 10081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 10141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 10201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 10261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 10321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 10381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 10441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 10501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 10561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 10621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 10681 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 10741 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 10801 ttccccgaaa agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt 10861 aaatttttta atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa 10921 aaatagtttt tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac 10981 cttttatctt gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg 11041 tagaagacca cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta 11101 tatctattta atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg 11161 aaatttttta aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt 11221 atttactttc taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc 11281 aaattattcc atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct 11341 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 11401 gtc //