LOCUS       Exported                9338 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  expresses a chimeric cMYC-repressor fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pSN29
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1242
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1309..2625
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    3205..3793
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3258..3278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3676..3687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3946..4126
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4198..4319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4235..4254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4289..4308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4359..4494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4421..4440
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4514..4533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4515..4533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4543..4560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4543..4559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4552..4574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4701..5156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4788..4807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4998..5019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5182..5286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5287..6147
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5505..5524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6318..6906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6807..6826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7060..7077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7194..7215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7230..7260
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7268..7284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7273..7295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7292..7308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7292..7308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7327..7347
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7329..7347
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7375..7601
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7602..7782
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7829..7954
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8447..8680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8865..8909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9176..9293
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      721 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1141 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactct cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat gcccctcaac
     1321 gttagcttca ccaacaggaa ctatgacctc gactacgact cggtgcagcc gtatttctac
     1381 tgcgacgagg aggagaactt ctaccagcag cagcagcaga gcgagctgca gcccccggcg
     1441 cccagcgagg atatctggaa gaaattcgag ctgctgccca ccccgcccct gtcccctagc
     1501 cgccgctccg ggctctgctc gccctcctac gttgcggtca cacccttctc ccttcgggga
     1561 gacaacgacg gcggtggcgg gagcttctcc acggccgacc agctggagat ggtgaccgag
     1621 ctgctgggag gagacatggt gaaccagagt ttcatctgcg acccggacga cgagaccttc
     1681 atcaaaaaca tcatcatcca ggactgtatg tggagcggct tctcggccgc cgccaagctc
     1741 gtctcagaga agctggcctc ctaccaggct gcgcgcaaag acagcggcag cccgaacccc
     1801 gcccgcggcc acagcgtctg ctccacctcc agcttgtacc tgcaggatct gagcgccgcc
     1861 gcctcagagt gcatcgaccc ctcggtggtc ttcccctacc ctctcaacga cagcagctcg
     1921 cccaagtcct gcgcctcgca agactccagc gccttctctc cgtcctcgga ttctctgctc
     1981 tcctcgacgg agtcctcccc gcagggcagc cccgagcccc tggtgctcca tgaggagaca
     2041 ccgcccacca ccagcagcga ctctgaggag gaacaagaag atgaggaaga aatcgatgtt
     2101 gtttctgtgg aaaagaggca ggctcctggc aaaaggtcag agtctggatc accttctgct
     2161 ggaggccaca gcaaacctcc tcacagccca ctggtcctca agaggtgcca cgtctccaca
     2221 catcagcaca actacgcagc gcctccctcc actcggaagg actatcctgc tgccaagagg
     2281 gtcaagttgg acagtgtcag agtcctgaga cagatcagca acaaccgaaa atgcaccagc
     2341 cccaggtcct cggacaccga ggagaatgtc aagaggcgaa cacacaacgt cttggagcgc
     2401 cagaggagga acgagctaaa acggagcttt tttgccctgc gtgaccagat cccggagttg
     2461 gaaaacaatg aaaaggcccc caaggtagtt atccttaaaa aagccacagc atacatcctg
     2521 tccgtccaag cagaggagca aaagctcatt tctgaagagg acttgttgcg gaaacgacga
     2581 gaacagttga aacacaaact tgaacagcta cggaactctt gtgcgggaaa gaaaaccaag
     2641 cggacagctg acagttcttc ttcagaggat gaggaggagt atgtcgtgga gaaggtgcta
     2701 gacaggcgcg tggttaaggg acaagtggaa tatctactga agtggaaagg cttttctgag
     2761 gagcacaata cttgggaacc tgagaaaaac ttggattgcc ctgagctaat ttctgaattt
     2821 atgaaaaagt ataagaagat gaaggagggt gaaaataata aacccaggga gaagtcagaa
     2881 agtaacaaga ggaaatccaa tttctcaaac agtgccgatg acatcaaatc taaaaaaaag
     2941 agagagcaga gcaatgatat cgctcggggc tttgagagag gactggaacc agaaaagatc
     3001 attggggcaa cagattcctg tggtgattta atgttcctaa tgaaatggaa agacacagat
     3061 gaagctgacc tggttcttgc aaaagaagct aatgtgaaat gtccacaaat tgtgatagca
     3121 ttttatgaag agagactgac atggcatgca tatcctgagg atgcggaaaa caaagagaaa
     3181 gaaacagcaa agagctaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3241 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3301 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3361 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3421 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3481 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3541 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     3601 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3661 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3721 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3781 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     3841 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3901 aacgaagaca agatatcctg ctagctccgg atccggacct gtactgggtc tctctggtta
     3961 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4021 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4081 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     4141 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     4201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4321 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4381 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4441 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     4501 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4681 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5041 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5101 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     5161 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5461 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5521 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6181 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6241 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6961 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7021 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7081 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7141 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7201 agttagctca ctcattaggc accccaggtt ttacacttta tgcttccggc tcgtatgttg
     7261 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7321 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     7381 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     7441 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     7501 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     7561 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     7621 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7681 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7741 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7801 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7861 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7921 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7981 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     8041 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     8101 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     8161 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     8221 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     8281 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     8341 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     8401 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     8461 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8521 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8581 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8641 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8701 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8761 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8821 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8881 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8941 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     9001 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     9061 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     9121 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     9181 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9241 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     9301 acgagactag cctcgagaag cttgatatcg aattccac
//
