LOCUS       Exported                9065 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  It contains ~4.8 Kb of the miR-183-96-182 cluster promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4-Cluster Promoter Delta 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Segura MF, Jubierre L, Li S, Soriano A, Koetz L, Gaziel-Sovran A, 
            Masanas M, Kleffman K, Dankert JF, Walsh MJ, Hernando E
  TITLE     Kruppel-like factor 4 (KLF4) regulates the miR-183~96~182 cluster 
            under physiologic and pathologic conditions.
  JOURNAL   Oncotarget. 2017 Apr 18;8(16):26298-26311. doi: 
            10.18632/oncotarget.15459.
  PUBMED    28412746
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     15..34
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4925..4956
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      4983..4992
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4989..6641
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    6685..6806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6696..6715)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6750..6769
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7054..7071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7225..7813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7305..7324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8013..8873)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    8978..9026
                     /note="synthetic polyadenylation signal"
     misc_feature    9040..66
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
       61 ttctctggcc taactggccg gtaccgagct cggatccact agtaacggcc gccagtgtgc
      121 tggaattcgc ccttgaggct ggactcgatc ctttaacctg gcaggccacc ttctcctctg
      181 acttgggtag gggacccctc tggggtggac gcttatgttt cacctgctcg ggggtcaaag
      241 tcgaccgggg tgtcgcctgg taaaaagcag caagggccgc acccgcacgc ttctctctcc
      301 tctcgccttt gctccgagcg acgattctct actcatcgag gattcatggg agccaagagc
      361 ccgggcgagg cccgccgccc cagtgcctcc gctctgcagc tcgccctctg attcaactcc
      421 tgcaggcttc agcatcccga atgcttattt tattttattt tatttcattt tatttattga
      481 gacggagttt cgttcttgtt gcccaggctg gagtgcaatg gcgcgatctc ggctcaccgc
      541 aacctcctct ttccgggttc aagcgattct cctgcctcag cctccggagt agctggcatt
      601 acaggcgagt gccgccacac ccggctaatt ttgtattttt agtagagatg ggctttctcc
      661 atgttagtca cgctggttgg tctcgaactt ctgatctcag gtgatccacc cgcctcggcc
      721 tcccaaagag ctgggattac aggcgtgagc cactgcgccc ggcccaggtt gctttttaaa
      781 aaatcaattt attggccggg cgcggtggct cacgcctgtg atcccaacac tttgggagac
      841 cgaggtgggc ggatcacgag gtcaggagat cgagaccatc ctgactaaca cggtgaaacc
      901 ccgcctctac taaaaaaaaa aaaaaaaaag aaaaaaggaa aaaaattagc cgggcgtggt
      961 ggcacgcgcc tgtaatccca gctactcggg agactgaggc aggagaattg cttgagccca
     1021 ggaggcggag gttgcagtga gcctaaatag cgccactgca ctccagtctg ggctacagag
     1081 cgagactccg tctcaaaaca aacaaacaaa caaaaaacaa ttcctgcagt ttcttttttt
     1141 aattgaaaat tcaatgcaag aatatggtaa gcaacacaca ttggcttcga acagtcccga
     1201 aagacataca atgaaaagaa aaatctccaa ccctctgcgc tgagaccttt cgtgcagaaa
     1261 aaacctgatg tttaaccccc tgccccattt atatttttac acaaatagga atccacgtgc
     1321 cttttgcgtt tttcactgaa cgcctaggcg actttccagg atatataaag accccatgtt
     1381 ttcctcattc ttttaaagag ctgggtcgat tccgaagaca gaatctcctt tcatttgaca
     1441 gtctctgtgt ttctccttct ttcgaatctc tgcatcctcc gaccccgcag ccgggaagga
     1501 accactccag cctcccgtcc aaacagaacc ctacagggcc ctacctcgat cagaggaaaa
     1561 acgcacagaa gcacacgcat acggttttcg aaaaatgtcg ggaagttcgt tgggtttcca
     1621 gtgactttgt tgacccacga gaatctagat gctagagcaa aggaccgagg gctaagggct
     1681 gagccaagat cgaaagactc ccgctttggc ttccccggca cgtggccctg gcggaggaag
     1741 caccagggac acagcggctg gggcgggcac ccggcggcag tcgcgcggac gagggtgcag
     1801 ggcgtgctga ggccatctcg ctgggcctgc agtttgcaga gatctccggg tcgccaagta
     1861 cccacgtgct cacatgtgca ttttcttcat ccccagaggt gcgagtcatt ctgcccattt
     1921 tgcaaatggg aaactgaggc ttagagaggt gaagtgaatc cctcgagatc gagatcacag
     1981 agggactgga gcaggggagg actggaactc tgggttcctc tcactggggt tatactagtt
     2041 atgccatggg gccagggtta gcaggagaac ctcaccattc ggagaggggc caatggcaga
     2101 accacggccg catcagtggg tctgcgtgag gggcttgtat cccgggcacc tttcccccta
     2161 gtcctgccgc cggaggaatg ggaagggagg ggaagaccga tggttacttc tttgcccaag
     2221 gtcttcagca cgagtgggat tgtcatggag ctggcgaagg gagccgtgtg gggtagggac
     2281 taggagggag caagccagag ggacaagccg cgacaccatt aaataggacg gagcgcaagg
     2341 cgccgagtct cctaggtggg ggcagcaagg aagacagcgg gtctttccta aacctggcca
     2401 agcgtcgagg aacccaaaac tacccgtccc ggggcgcacg actccgggca gcgtgggacg
     2461 cctgtggcga ggcggaaggg atctctccag cgagagggaa tgcggcaatt gccagacgga
     2521 cattggatct gcagaaggag ggggtaggat acacgcgggc ttaggatgcg ctggacaggc
     2581 cagcaaagtg aggaccccag gtcgtcctga gccacctctt cccccgcctg agccctgagc
     2641 cgtaagggat ccagggtatc aggtccccgg gggccgcccc ctcaccgtcc gagtccagct
     2701 ccggttacct gcctggcctg ctccttaaaa tatgtatgag gcccttgcgg gtgcagtttc
     2761 gcggcccctc ccggagtggg gctgggggac tcagggtgcg aggggagctg aggggggagg
     2821 aagcgacgcg agctccccgg ccggggcctt ctcgactttc agcagcgcat ttaagaaaag
     2881 gtggaaatcg gatctgtctc ggcggcgccc cagccccgcc cgctccgacg gccggacctc
     2941 gcggcccagc ggctagcttt ctgctgcgcg gtgtctggtt ctcgacagca ggtgaatggg
     3001 ccagggcggg gcgcccggag cgcggggggc ggggcgcgcg ttataaaggg gagcagcagg
     3061 ccgcgggggc cactcgcagt ccgcaaggcc tgcgcttccc gctttgcggt cctgggcaca
     3121 cccggcccgg gttgcccagc atcgcatggg tctgaggacc aggtagggcg cgggtgcaga
     3181 gacagccagg gaggctcgag gcgacctaga tgggcggggg aatgctagta gggcggggag
     3241 gggttaatgt ggagctatca cccccttttg gcccgttggg ctctgtgacg tgctcattat
     3301 ctccgctgtc cgggttctcg ctcaccgtgc ccatccctgg ctgcatgggg agaggggccc
     3361 cgaggccctg agactcgtcc agttatccac ctggggtggg aatcttgctt cccgatccat
     3421 ctggctgcga catctacggc ttcagcaggc cctgcccggg agtcgggcat attgaagggt
     3481 tcgtatcctt gccgtcaccc ttggcccctc ccggctcggc aggagctggg ggtggggaaa
     3541 ctatagattt cccgggcccc gcttccactg cctcaccccc ttcttttgct cgagcccaag
     3601 acaaggtgtt aacggtcttc aagtgcaagg gttaggtttg gtgggagcat aggctaagca
     3661 ccaccctcat tttacagggg cttctagggt gccagatctg cgggggtcga ttgggaagaa
     3721 ctagaagcta aaaaggagaa gggcggatta acgttttatt ttaatctggt tttgagctgc
     3781 cgttgccgtc tggggcggcc gggcggggcg gagctgggac tgccaagggc agccaaagca
     3841 gcagggaggg agggggcgct ccggtgctca ggatgctggg gaagaaggaa gaggccaccc
     3901 caacgctgac agcttggagg acctagcccc tgccccccgg tcccttctga atgctccggg
     3961 tggtgtcaga ccaaaggacg gttgagaagg atctaatccg cgtccgcgcc ccagccccgg
     4021 ctggccccgc cccctcccga gggagccact gtcgcagtgg ggggaggggg agatgggaac
     4081 gggtctgggg gcggctcccc tccccctccc tctcttggga cagacagcct ttgttcccgg
     4141 cggcggcggc agcggtggcc agacacctgt gaggccgcag aactggccga gggtgggcgt
     4201 gtgaggacat ggaggcccct tgtccccatt ccagccctgc gcccctgggt ccgggtgcgc
     4261 cccctgagac aggtcagcct tgagcacctt tctgcgggga gactgcagag atcgtccagt
     4321 ggccctacgg tgcgccccag ctcccaacgc acttcccctc ccccctccca gggtcggatt
     4381 tccaagcacg ccacgccaca cacacctcgg ctagctccgg gctcagaggg cgctggggtg
     4441 gcagggcgtc cgggcgcacc tcggctagcg gcaccctagc ccgagggcag gaggcgggga
     4501 tagaaggcgg gcgacgggac cccttggggc cagacccggc cgggttgggc aagcaggcgg
     4561 ggctcccggc gtctccgcag tcccggacgg gcgtgccctg cgccgcgcac caaccggagc
     4621 cacattcaag gtgaatcatc cccggcccgc cccctgcccc tccccgcctc gcggcacctg
     4681 agccaatggc gctgcgcgag gggcgggcgg ggcccggggc ggggggcggt aaaaccctcc
     4741 tggagtcatt ctctgtgcac tccgtcgagc ccccgcgcag ccttgccgga gccgcagggc
     4801 ctccgtccag ccgcgcggtc cgggcagccc gcgccccctg ttcttccggt cctcgggccc
     4861 aggtacctga gctcgctagc ctcgaggata tcaagatctg gcctcggcgg ccaagcttag
     4921 acactagagg gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta
     4981 aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg
     5041 aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg
     5101 gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg
     5161 agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga
     5221 tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca
     5281 tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca
     5341 tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca
     5401 acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact
     5461 accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg
     5521 agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca
     5581 gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc
     5641 gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc
     5701 tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct
     5761 gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc
     5821 aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga
     5881 gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc
     5941 cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc
     6001 agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca
     6061 agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca
     6121 ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca
     6181 tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc
     6241 tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc
     6301 tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc
     6361 tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg
     6421 gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga
     6481 tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt
     6541 tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga
     6601 ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag agtcggggcg
     6661 gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     6721 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6781 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     6841 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     6901 tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     6961 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     7021 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7981 cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag tgaggcaccg
     8041 atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt cgtgtagatc
     8101 actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc gcgagagccg
     8161 cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc cgagcgaaga
     8221 agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg tgatgctaga
     8281 gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac tggcatcgtg
     8341 gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg gtcaagccgg
     8401 gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc tccgatcgtt
     8461 gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact acacaattct
     8521 cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc aaccaagtcg
     8581 ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat acgggacaac
     8641 accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc ttcggggcgg
     8701 aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac tcttgcaccc
     8761 agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa aacaggcaag
     8821 caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct catactcgtc
     8881 ctttttcaat attattgaag catttatcag ggttactagt acgtctctca aggataagta
     8941 agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt tattttcatt
     9001 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
     9061 caaaa
//
