LOCUS Exported 6008 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Baculovirus expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS DPAGT1 (DPAGT1A-c005) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6008) TITLE SGC Oxford 2017 REFERENCE 2 (bases 1 to 6008) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6008 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..92 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 17..34 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 154..171 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 196..216 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" polyA_signal 1614..1748 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1651..1670) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1705..1724 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 1777..1942 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" rep_origin 2126..2580 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2213..2232) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2422..2443 /label=F1ori-F /note="F1 origin, forward primer" promoter 2606..2710 /gene="bla" /label=AmpR promoter CDS 2711..3571 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2929..2948) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3742..4330 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4231..4250 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4484..4501 /label=L4440 /note="L4440 vector, forward primer" mobile_element 4635..4859 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(5629..5657) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" ORIGIN 1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc 61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca 121 tcgggcgcgg atctcggtcc gaaaaccatg ggccaccatc atcatcatca ttcttctggt 181 gtagatctgg gtaccgagaa cctgtacttc caatccatgt gggccttctc ggaattgccc 241 atgccgctgc tgatcaattt gatcgtctcg ctgctgggat ttgtggccac agtcaccctc 301 atcccggcct tccggggcca cttcattgct gcgcgcctct gtggtcagga cctcaacaaa 361 accagccgac agcagatccc agaatcccag ggagtgatca gcggtgctgt tttccttatc 421 atcctcttct gcttcatccc tttccccttc ctgaactgct ttgtgaagga gcagtgtaag 481 gcattccccc accatgaatt tgtggccctg ataggtgccc tccttgccat ctgctgcatg 541 atcttcctgg gctttgcgga tgatgtactg aatctgcgct ggcgccataa gctgctgcta 601 cctacagctg cctcactacc tctcctcatg gtctatttca ccaactttgg caacacgacc 661 attgtggtgc ccaagccctt ccgcccgata cttggcctgc atctggactt gggaatcctg 721 tactatgtct acatggggct gctggcagtg ttctgtacca atgccatcaa tatcctagca 781 ggaattaacg gcctagaggc tggccagtca ctagtcattt ctgcttccat cattgtcttc 841 aacctggtag agttggaagg tgattgtcgg gatgatcatg tcttttccct ctacttcatg 901 ataccctttt ttttcaccac tttgggattg ctctaccaca actggtaccc atcacgggtg 961 tttgtgggag ataccttctg ttactttgct ggcatgacct ttgccggggt gggcatcttg 1021 ggacacttca gcaagaccat gctactattc ttcatgcccc aggtgttcaa cttcctctac 1081 tcactgcctc agctcctgca tatcatcccc tgccctcgcc accgcatacc cagactcaat 1141 atcaagacag gcaaactgga gatgagctat tccaagttca agaccaagag cctctctttc 1201 ttgggcacct ttattttaaa ggtggcagag agcctccagc tggtgacagt acaccagagt 1261 gagactgaag atggtgaatt cactgaatgt aacaacatga ccctcatcaa cttgctactt 1321 aaagtccttg ggcccataca tgagagaaac ctcacattgc tcctgctgct gctgcagatc 1381 ctgggcagtg ccatcacctt ctccattcga tatcagctcg ttcgactctt ctatgatgtc 1441 tgacagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt gtcgagaagt 1501 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 1561 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 1741 tctggatctg atcactgata tcgcctagga gatccgaacc agataagtga aatctagttc 1801 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat 1861 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc 1921 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac 1981 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat 2041 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt 2101 atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt 2161 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2221 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2281 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 2341 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 2401 ttggagtcca cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2461 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2521 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt 2581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 2641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 2701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 2761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 2821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 2881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 2941 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 3001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 3061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 3121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 3181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 3241 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 3301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 3361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 3421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 3481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 3541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 3601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 3661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 3721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 3901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 4021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 4081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 4141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 4441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 4501 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 4561 ccgcatagac cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc 4621 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 4681 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 4741 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 4801 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg 4861 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg 4921 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc 4981 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca 5041 taagcaccaa gcgcgttggc ctcatgcttg agcagattga tgagcgcggt ggcaatgccc 5101 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc 5161 tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca 5221 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga 5281 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc 5341 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca 5401 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc 5461 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca 5521 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg 5581 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc 5641 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg 5701 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag 5761 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg 5821 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg 5881 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat 5941 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat 6001 attaatag //