LOCUS Exported 11358 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression plasmid for N-terminal HA-tagged human CCR5. ACCESSION . VERSION . KEYWORDS pCEP4-HA-CCR5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11358) AUTHORS Heredia JD, Park J, Brubaker RJ, Szymanski SK, Gill KS, Procko E TITLE Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. JOURNAL J Immunol. 2018 Apr 20. pii: jimmunol.1800343. doi: 10.4049/jimmunol.1800343. PUBMED 29678950 REFERENCE 2 (bases 1 to 11358) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11358 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 475..2264 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(2570..4495) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(4930..4948) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5016..5120 /gene="bla" /label=AmpR promoter CDS 5121..5981 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5339..5358) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6152..6740 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6641..6660 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 7171..7316 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 7359..8396 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" primer_bind complement(8394..8413) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 8438..8486 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" enhancer 9140..9519 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9520..9723 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9674..9694 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 9720..9744 /label=LNCX /note="Human CMV promoter, forward primer" CDS 9826..9852 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" protein_bind 10018..10029 /label=ZF binding site /bound_moiety="CCR5TC zinc-finger domain" /note="target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)" polyA_signal 10949..11083 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10973..10992) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 11027..11046 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(11225..11247) /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gccccgccgc cggacgaact aaacctgact acggcatctc tgccccttct tcgctggtac 61 gaggagcgct tttgttttgt attcggggca gtgcatgtaa tcccttcagt tggttggtac 121 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt 181 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc 241 tttcatcctg gagcagactt tgcatgctgt ggactgcaac acaacattgc ctttatgtgt 301 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg 361 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg 421 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg 481 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat 541 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa 601 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca 661 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt 721 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact 781 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc 841 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata 901 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct 961 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac 1021 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga 1081 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag 1141 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa 1201 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt 1261 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac 1321 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac 1381 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg 1441 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga 1501 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct 1561 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc 1621 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc 1681 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc 1741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc 1801 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt 1861 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata 1921 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata 1981 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata 2041 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata 2101 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata 2161 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc 2221 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgcatgct ggttgctccc 2281 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag 2341 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg 2401 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat 2461 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg 2521 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc 2581 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg 2641 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc 2701 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa 2761 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga 2821 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga 2881 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt 2941 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt 3001 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct 3061 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc 3121 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg 3181 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt 3241 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag 3301 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct 3361 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt 3421 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc 3481 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg 3541 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc 3601 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc 3661 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc 3721 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc 3781 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc 3841 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc 3901 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc 3961 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg 4021 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg 4081 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc 4141 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc 4201 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc 4261 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc 4321 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat 4381 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt 4441 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc 4501 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc 4561 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac 4621 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact 4681 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag 4741 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc 4801 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg 4861 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa 4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 5341 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 5641 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 6781 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca 6841 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc 6901 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt 6961 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt 7021 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag 7081 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa 7141 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca 7201 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat 7261 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc 7321 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg 7381 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc 7441 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc 7501 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg 7561 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga 7621 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac 7681 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta 7741 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc 7801 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg 7861 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact 7921 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca 7981 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg 8041 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct 8101 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc 8161 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt 8221 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac 8281 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata 8341 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca 8401 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 8461 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 8521 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 8581 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 8641 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta 8701 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag 8761 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg 8821 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt 8881 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg 8941 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg 9001 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc 9061 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt 9121 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg 9181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 9241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 9301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 9361 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat 9421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact 9481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac 9541 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac 9601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac 9661 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga 9721 gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg 9781 aagaccatca tcgccctgag ctacatcttc tgcctggtgt tcgcctaccc ctacgacgtg 9841 cccgactacg ccggctcccc gggaggcgcg gattatcaag tgtcaagtcc aatctatgac 9901 atcaattatt atacatcgga gccctgccaa aaaatcaatg tgaagcaaat cgcagcccgc 9961 ctcctgcctc cgctctactc actggtgttc atctttggtt ttgtgggcaa catgctggtc 10021 atcctcatcc tgataaactg caaaaggctg aagagcatga ctgacatcta cctgctcaac 10081 ctggccatct ctgacctgtt tttccttctt actgtcccct tctgggctca ctatgctgcc 10141 gcccagtggg actttggaaa tacaatgtgt caactcttga cagggctcta ttttataggc 10201 ttcttctctg gaatcttctt catcatcctc ctgacaatcg ataggtacct ggctgtcgtc 10261 catgctgtgt ttgctttaaa agccaggacg gtcacctttg gggtggtgac aagtgtgatc 10321 acttgggtgg tggctgtgtt tgcgtctctc ccaggaatca tctttaccag atctcaaaaa 10381 gaaggtcttc attacacctg cagctctcat tttccataca gtcagtatca attctggaag 10441 aatttccaga cattaaagat agtcatcttg gggctggtcc tgccgctgct tgtcatggtc 10501 atctgctact cgggaatcct aaaaactctg cttcggtgtc gaaatgagaa gaagaggcac 10561 agggctgtga ggcttatctt caccatcatg attgtttatt ttctcttctg ggctccctac 10621 aacattgtcc ttctcctgaa caccttccag gaattctttg gcctgaataa ttgcagtagc 10681 tctaacaggt tggaccaagc tatgcaggtg acagagactc ttgggatgac gcactgctgc 10741 atcaacccca tcatctatgc ctttgtcggg gagaagttca gaaactacct cttagtcttc 10801 ttccaaaagc acattgccaa acgcttctgc aaatgctgtt ctattttcca gcaagaggct 10861 cccgagcgag caagctcagt ttacacccga tccactgggg agcaggaaat atctgtgggc 10921 ttgtgataac tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt 10981 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc 11041 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat 11101 tcattttatg tttcaggttc agggggaggt ggggaggttt tttaaagcaa gtaaaacctc 11161 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg 11221 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 11281 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 11341 tgaccggtcg actctaga //