LOCUS       Exported                 853 bp ds-DNA     linear   SYN 01-JUN-2021
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    DCT.VV.Orf270
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             718..759
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             769..786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(809..826)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctcggat ccgtaccctt caccatgtct cgtggggcat taatcgtttt tgaaggattg
       61 gacaaatctg gaaaaacaac acaatgtatg aacatcatgg aatctatacc ggcaaacacg
      121 ataaaatatc ttaactttcc tcagagatcc actgtcactg gaaagatgat agatgactat
      181 ctaactcgta aaaaaaccta taatgatcat atagttaatc tattattttg tgcaaataga
      241 tgggagtttg catcttttat acaagaacaa ctagaacagg gaattacttt aatagttgat
      301 agatacgcgt tctctggagt agcgtatgcc gccgctaaag gcgcgtcaat gactctcagt
      361 aagagttatg aatctggatt gcctaaaccc gacttagtta tattcttgga atctggtagc
      421 aaagaaatta atagaaacgt cggcgaggaa atttatgaag atgttacatt ccaacaaaag
      481 gtattacaag aatataaaaa aatgattgaa gaaggagata ttcattggca aattatttct
      541 tctgaattcg aggaagatgt aaagaaggag ttgattaaga atatagttat agaggctata
      601 cacacggtta ctggaccagt ggggcaactg tggatgtaag gatccaaggg tcaagacaat
      661 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
      721 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
      781 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      841 gttgtttgcc cct
//
