LOCUS       Exported                9083 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  expresses a chimeric OCT4-activator fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pSN34
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M
  TITLE     OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming 
            Human Fibroblasts.
  JOURNAL   Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 
            10.1016/j.celrep.2017.07.071.
  PUBMED    28813671
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..511
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     430..448
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             526..1242
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(571..592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(832..851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1179..1200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1252..1308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        complement(2930..2947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2971..3559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3024..3044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3442..3453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3712..3892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3964..4085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4001..4020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4055..4074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4104..4239
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4166..4185
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4259..4278)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4260..4278)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4288..4305)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4288..4304)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4297..4319)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4446..4901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4533..4552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4743..4764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4927..5031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5032..5892
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5250..5269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6063..6651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6552..6571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6805..6822
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6939..6960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6975..7005
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7013..7029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7018..7040
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7037..7053
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7037..7053
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7072..7092
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7074..7092
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7120..7346
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7347..7527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7574..7699
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8192..8425
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8610..8654
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8921..9038
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtatggt gagcaagggc
      541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      601 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      721 agctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc
      841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      961 tacaacagcc acaacgtcta tatcacggcc gacaagcaga agaacggcat caaggcgaac
     1021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gttcatccag
     1141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1201 accgccgccg ggatcactca cggcatggac gagctgtaca agggatctgg agcaacaaac
     1261 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggcgggacac
     1321 ctggcttcgg atttcgcctt ctcgccccct ccaggtggtg gaggtgatgg gccagggggg
     1381 ccggagccgg gctgggttga tcctcggacc tggctaagct tccaaggccc tcctggaggg
     1441 ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt gggggattcc cccatgcccc
     1501 ccgccgtatg agttctgtgg ggggatggcg tactgtgggc cccaggttgg agtggggcta
     1561 gtgccccaag gcggcttgga gacctctcag cctgagggtg aagcaggagt cggggtggag
     1621 agcaactccg atggggcctc cccggagccc tgcaccgtca cccctggtgc cgtgaagctg
     1681 gagaaggaga agctggagca aaacccggag gagtcccagg acatcaaagc tctgcagaaa
     1741 gaactcgagc aatttgccaa gctcctgaag cagaagagga tcaccctggg atatacacag
     1801 gccgatgtgg ggctcaccct gggggttcta tttgggaagg tattcagcca aacgaccatc
     1861 tgccgctttg aggctctgca gcttagcttc aagaacatgt gtaagctgcg gcccttgctg
     1921 cagaagtggg tggaggaagc tgacaacaat gaaaatcttc aggagatatg caaagcagaa
     1981 accctcgtgc aggcccgaaa gagaaagcga accagtatcg agaaccgagt gagaggcaac
     2041 ctggagaatt tgttcctgca gtgcccgaaa cccacactgc agcagatcag ccacatcgcc
     2101 cagcagcttg ggctcgagaa ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag
     2161 ggcaagcgat caagcagcga ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct
     2221 ttctcagggg gaccagtgtc ctttcctctg gccccagggc cccattttgg taccccaggc
     2281 tatgggagcc ctcacttcac tgcactgtac tcctcggtcc ctttccctga gggggaagcc
     2341 tttccccctg tctctgtcac cactctgggc tctcccatgc attcaaacgg atccgttagt
     2401 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg
     2461 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaga
     2521 tccgcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg
     2581 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat
     2641 tccccgggtc cgagatccgt tagtatgcag ccgtcactta gaagtgagta tgagtaccct
     2701 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt
     2761 gatgcggaga gatgggtaag cagatccgcc cccccgaccg atgtcagcct gggggacgag
     2821 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
     2881 ctggacatgt tgggggacgg ggattccccg ggtccgagat cctctagaac tatagtgagt
     2941 cgtattacgt gagcggccgc gtaggtcgac aatcaacctc tggattacaa aatttgtgaa
     3001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3361 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3541 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     3601 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3661 actcccaacg aagacaagat atcctgctag ctccggatcc ggacctgtac tgggtctctc
     3721 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3781 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3841 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     3901 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     3961 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4021 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4081 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     4141 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4201 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     4261 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     4321 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4381 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4441 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4501 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4561 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4621 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4681 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4741 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4801 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4861 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4921 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4981 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5041 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5101 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5161 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5221 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5281 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5341 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5401 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5461 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5521 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5581 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5641 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5701 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5761 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5821 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5881 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5941 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6001 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6181 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6421 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6901 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6961 taggcacccc aggttttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7021 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     7081 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     7141 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     7201 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     7261 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     7321 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     7381 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7441 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7501 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     7561 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     7621 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     7681 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     7741 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     7801 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7861 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7921 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7981 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     8041 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     8101 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     8161 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     8221 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     8281 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     8341 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     8401 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     8461 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     8521 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     8581 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     8641 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     8701 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     8761 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     8821 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     8881 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
     8941 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9001 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctcg
     9061 agaagcttga tatcgaattc cac
//
