LOCUS Exported 10240 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Labels mother centrioles/basal bodies. ACCESSION . VERSION . KEYWORDS pLenti-EGFP-hChibby1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10240) TITLE pLenti-EGFP-hChibby1 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10240) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10240 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 1190..1906 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1190..1906 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1235..1256) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1496..1515) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1843..1864 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2330..2918 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2383..2403) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2801..2812) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3442..3622 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(3648..3665) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3654..3878 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3924..4352 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4011..4030) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4221..4242 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4361..4381) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4366..4695 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4546..4681 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4608..4627 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 4743..4790 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 4809..5183 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 5313..5434 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5350..5369) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5404..5423 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5483..5499) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5483..5499) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5496..5518) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5507..5523 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5531..5561) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5576..5597 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5714..5731) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5885..6473) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5965..5984) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6644..7504) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7267..7286 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7505..7609) /gene="bla" /label=AmpR promoter primer_bind complement(7684..7703) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 7875..8254 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8256..8454 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 8409..8429 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 8472..8652 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8699..8824 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9317..9550 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9735..9779 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10077..10194 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgacca tggtgagcaa 1201 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 1261 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 1321 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 1381 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 1441 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 1501 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 1561 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 1621 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 1681 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 1741 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 1801 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 1861 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaat tcggggccat 1921 tacggccatg cctttctttg ggaatacgtt cagtccgaag aagacacctc ctcggaagtc 1981 ggcatctctc tccaacctgc attctttgga tcgatcaacc cgggaggtgg agctgggctt 2041 ggaatacgga tccccgacta tgaacctggc agggcaaagc ctgaagtttg aaaatggcca 2101 gtggatagca gagacagggg ttagtggcgg tgtggaccgg agggaggttc agcgccttcg 2161 caggcggaac cagcagttgg aggaagagaa caatctcttg cggctgaaag tggacatctt 2221 attagacatg ctttcagagt ccactgccga atcccactta atggagaagg aactggatga 2281 actgaggatc agccggaaga gaaaatgagg ccgcctcggc cgcggccgca atcaacctct 2341 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 2401 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 2461 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 2521 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 2581 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 2641 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 2701 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 2761 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 2821 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 2881 tcagacgagt cggatctccc tttgggccgc ctccccgcct gcctgcaggt ttgtcgagac 2941 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 3001 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 3061 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 3121 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 3181 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca 3241 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc 3301 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg 3361 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga 3421 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 3481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 3541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 3601 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc 3661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 3721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 3781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 3841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 3901 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 3961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 4021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 4081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 4141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 4201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 4261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 4321 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 4381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 4561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 4621 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4681 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac 4741 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga 4801 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg 4861 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact 4921 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg 4981 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt 5041 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg 5101 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact 5161 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct 5221 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 5281 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 5341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 5401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 5461 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 5521 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 5581 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 5641 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 5701 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 5941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 6241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 6301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 6361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 6421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 6481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 6541 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 6601 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6661 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6721 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6781 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6841 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6901 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 6961 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7021 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7081 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7141 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7201 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 7261 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 7321 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 7381 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 7441 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7501 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7561 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 7621 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct 7681 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 7741 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 7801 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 7861 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 7921 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 7981 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 8041 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 8101 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 8161 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 8221 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa 8281 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa 8341 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc 8401 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt 8461 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 8521 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 8581 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 8641 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct 8701 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact 8761 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag 8821 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg 8881 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt 8941 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct 9001 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac 9061 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat 9121 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac 9181 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa 9241 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa 9301 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta 9361 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc 9421 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag 9481 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc 9541 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt 9601 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg 9661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 9721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 9781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 9841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 9901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 9961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga 10021 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt 10081 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca 10141 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt 10201 tattacaggg acagcagaga tccagtttgg ttaattaatt //