LOCUS Exported 2245 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSF. ACCESSION . VERSION . KEYWORDS BB2_L_12_syn_A_F SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2245) AUTHORS Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG TITLE An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. JOURNAL Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. PUBMED 28533066 REFERENCE 2 (bases 1 to 2245) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2245 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..17 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(304..320) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(304..320) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 368..399 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 400..502 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 503..1363 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(721..740) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" terminator 1579..1606 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1618..2205) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1698..1717) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2245..17 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" ORIGIN 1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt 61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata 121 tgcggcctcg atcgaagacg cggagcgcaa aaaaccccgc ccctgacagg gcggggtttt 181 ttcgcgatcg gagaccggat ccctcgaggg tctccgcttc atggcgtctt cccgggcgcg 241 tgatcttacg gcattatacg tatgatcggt ccacgatcag ctagattatc tagtcagctt 301 gatgtcatag ctgtttcctg aggctcaata ctgaccattt aaatcatacc tgacctccat 361 agcagaaagt caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc 421 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt 481 ttcaggagct aaggaagcta aaatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agtttacgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atctcacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctggcaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga tcacttctgc gctcggccct cccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgcatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taatgagggc ccaaatgtaa 1381 tcacctggct caccttcggg tgggcctttc ttgaggacct aaatgtaatc acctggctca 1441 ccttcgggtg ggcctttctt gaggacctaa atgtaatcac ctggctcacc ttcgggtggg 1501 cctttcttga ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttcttgagg 1561 acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt 1621 ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg 1681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 2041 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc 2101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 2161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt 2221 ttctaccgaa gaaaggccca cccgt //