LOCUS Exported 2855 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION AAV expression of GFP spaghetti monster myc construct. ACCESSION . VERSION . KEYWORDS pAAV.CAG.GFPsm-myc.WPRE.SV40 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2855) AUTHORS Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger LL TITLE High-performance probes for light and electron microscopy. JOURNAL Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 2015 Apr 27. PUBMED 25915120 REFERENCE 2 (bases 1 to 2855) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2855 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(8..596) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(88..107) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(767..1627) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1390..1409 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1628..1732) /gene="bla" /label=AmpR promoter rep_origin complement(1758..2213) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1895..1916) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2107..2126 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 2285..2414 /label=AAV2 ITR ORIGIN 1 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 61 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 1021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 1261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 1321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 1381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 1441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 1501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 1561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 1621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 1681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 1741 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 1801 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 1861 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 1921 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 1981 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 2041 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 2101 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 2161 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc 2221 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 2281 ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac 2341 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat 2401 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg 2461 ctctaggaag agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 2521 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 2581 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 2641 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 2701 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 2761 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 2821 cgatgggggc gggggggggg ggggggcgcg cgcca //