LOCUS Exported 10044 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression. ACCESSION . VERSION . KEYWORDS pSin wPGK-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10044) AUTHORS Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E TITLE Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. JOURNAL Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. PUBMED 26853302 REFERENCE 2 (bases 1 to 10044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10044 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(3..502) /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(51..70) /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 444..467 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" CDS complement(801..845) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1030..1263 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 1756..1881 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 1928..2561 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" polyA_signal 2729..2863 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2753..2772) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2807..2826 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(3288..3308) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 3438..3503 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" promoter complement(4637..4966) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4651..4786 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4705..4724) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 4951..4971 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" primer_bind complement(5041..5058) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5212..5800) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5292..5311) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5971..6831) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6594..6613 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6832..6936) /gene="bla" /label=AmpR promoter primer_bind 7004..7022 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(7060..7082) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 7182..7201 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7415..7434 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 7724..7742 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 7724..7741 /label=SP6 /note="SP6 promoter, forward primer" LTR 7767..8000 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" misc_feature 8089..8677 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 8195..8206 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 8604..8624 /label=WPRE-R /note="WPRE, reverse primer" primer_bind 8690..8706 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 8691..8707 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS complement(9764..9784) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 9853..9970 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc 61 gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc 121 ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa 181 agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc 241 atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc 301 tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca 361 ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg 421 ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt 481 gggaaaagcg cctcccctac ccggtagaat agatccgatg gtcgaggcta gtctcgtgat 541 cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct 601 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac 661 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt 721 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt 781 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca 841 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg 901 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta 961 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc 1021 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg 1081 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc 1141 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc 1201 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct 1261 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct 1321 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc 1381 ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg tggtcttact 1441 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct 1501 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc 1561 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa 1621 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa 1681 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct 1741 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa 1801 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag 1861 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg 1921 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca 1981 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc 2041 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag 2101 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc 2161 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca 2221 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa 2281 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg 2341 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc 2401 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg 2461 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc 2521 aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac 2581 atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga 2641 gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat 2701 gattttggtt agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt 2761 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc 2821 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat 2881 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct 2941 ctacaaatgt ggtatggctg attatgatct ctagtcaagg cactatacat caaatattcc 3001 ttattaaccc ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta 3061 atagcagaca ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg 3121 ttatgcctac ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca 3181 gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca 3241 tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc 3301 ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt 3361 tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca 3421 gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat 3481 acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat 3541 tatgtcacac cacagaagta aggttccttc acaaagatcc ggggcccact cataaatcca 3601 gttgccgcca cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc 3661 gctcatcaca atacgtgcct ggacgtcgag gatttcgcgt gggtcaatgc cgcgccagat 3721 ccacatcaga cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga 3781 ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg 3841 tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc 3901 cagaatactt actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca 3961 gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat 4021 cccacggctg ttcaatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg 4081 gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa 4141 tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat 4201 cgccttctgc gcgtttcagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa 4261 tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacag 4321 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc 4381 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg 4441 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc 4501 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta 4561 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg 4621 tcttgtgtcc aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct 4681 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc 4741 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg 4801 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg 4861 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc 4921 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagctgca 4981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 5041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5641 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 5881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 5941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 6241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 6301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 6361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 6421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 6481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 6541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 6601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 6661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 6721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 6781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 6841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 6901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 6961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 7021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 7081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 7141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 7201 actgagagtg caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca 7261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg 7321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca 7381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag 7441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc 7501 tagcgatgac cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag 7561 aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata 7621 gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac 7681 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata 7741 gaatacacgg aattaattct agatgctgct agagattttc cacactgact aaaagggtct 7801 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca 7861 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc 7921 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg 7981 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc 8041 tgccttgtaa gtcattggtc ttaaaggtac cgagctcgaa ttcccgatgc ggggaggcgg 8101 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag 8161 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga 8221 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca 8281 gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc 8341 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc 8401 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca 8461 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca 8521 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca 8581 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag 8641 tcaatctttc acaaattttg taatccagag gttgattatc ggaattccct cgaggtcgac 8701 ggtatcgata agcttgatct agtggatccc cccgcgttaa tggctaatcg ccatcttcca 8761 gcaggcgcac cattgcccct gtttcactat ccaggttacg gatatagttc atgacaatat 8821 ttacattggt ccagccacca gcttgcatga tctccggtat tgaaactcca gcgcgggcca 8881 tatctcgcgc ggctccgaca cgggcactgt gtccagacca ggccaggtat ctctgaccag 8941 agtcatcctt agcgccgtaa atcaatcgat gagttgcttc aaaaatccct tccagggcgc 9001 gagttgatag ctggctggtg gcagatggcg cggcaacacc attttttctg acccggcaaa 9061 acaggtagtt attcggatca tcagctacac cagagacgga aatccatcgc tcgaccagtt 9121 tagttacccc caggctaagt gccttctcta cacctgcggt gctaaccagc gttttcgttc 9181 tgccaatatg gattaacatt ctcccaccgt cagtacgtga gatatcttta accctgatcc 9241 tggcaatttc ggctatacgt aacagggtgt tataagcaat ccccagaaat gccagattac 9301 gtatatcctg gcagcgatcg ctattttcca tgagtgaacg aacctggtcg aaatcagtgc 9361 gttcgaacgc tagagcctgt tttgcacgtt caccggcatc aacgttttct tttcggatcc 9421 gccgcataac cagtgaaaca gcattgctgt cacttggtcg tggcagcccg gaccgacgat 9481 gaagcatgtt tagctggccc aaatgttgct ggatagtttt tactgccaga ccgcgcgcct 9541 gaagatatag aagataatcg cgaacatctt caggttctgc gggaaaccat ttccggttat 9601 tcaacttgca ccatgccgcc cacgaccggc aaacggacag aagcattttc caggtatgct 9661 cagaaaacgc ctggcgatcc ctgaacatgt ccatcaggtt cttgcgaacc tcatcactcg 9721 ttgcatcgac cggtaatgca ggcaaatttt ggtgtgacct gcataccttt ctcttcttct 9781 taggtgtcat acctaagctg ggctgcagcc cgatccccaa agtggatctc tgctgtccct 9841 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat 9901 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt 9961 tcttttaaaa ttgtggatga atactgccat ttgtagatcc actagtaacg gccgccagtg 10021 tgctggaatt cggcttgcaa gctt //