LOCUS       Exported                1003 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Expression of EphA2.
ACCESSION   .
VERSION     .
KEYWORDS    pCLXSN-EphA2-Flag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Song W, Ma Y, Wang J, Brantley-Sieders D, Chen J
  TITLE     JNK signaling mediates EPHA2-dependent tumor cell proliferation, 
            motility, and cancer stem cell-like properties in non-small cell 
            lung cancer.
  JOURNAL   Cancer Res. 2014 May 1;74(9):2444-54. doi: 
            10.1158/0008-5472.CAN-13-2136. Epub 2014 Mar 7.
  PUBMED    24607842
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(26..73)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             complement(74..88)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(101..148)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 cnnnnngnng gtgatgatga ccggtcttat cgtcatcgtc tttgtaatcc ttgtcatcgt
       61 catctttata gtccttatcg tcgtcatcgt ctttgtaatc cttgtcatcg tcatctttat
      121 agtccttatc gtcgtcatcc ttgtaatctc tagagatggg gatccccaca gtgttcacct
      181 ggtccttgag tcccagcagg ctgtaggcga tgcgcttctg gtggccgggc agccgcaccc
      241 caatcctctt gatgtcgtcg ttggtcatct gcaccacctt ctcgatggca gtgtagccgg
      301 ccgccatgaa gtgctccgta tactgctgca tcttgatgga ctccagccac tcggacaccg
      361 tgcggaaggg caccccctcc gagccgctcg tgctggggag ccggatagac acgcgggggt
      421 caaagtcagc cagggtcttg agggagtcag gggcacgaat gagcttgtcc aggatgctga
      481 cgatgtcagc gaacttgggg cggcgggcac gctcctgctg ccagcactgc atcatgagct
      541 ggtagatggc ggangggcag tccatgggtg tggggagccg gaagccatca ttgatggctt
      601 tcatcacctc gtggttggac aactcccagt agggccgctc gccataggtc atcacctccc
      661 acatgacaat gccaaagctc cacacgtcgc tggcagaggt gaacttccgg taggaaatgg
      721 cctccggggc ggtccagcgg atggggatct tgccgccact ggtggtgtag gtggcctcgg
      781 ggtcgtcctc cagcacgcgg gacaggccaa agtcagacac cttgcagacc angttgctgt
      841 tgacgaggat gttgcgggca gccaggtcac ggtgcacata gttcatgttg ggccaggtac
      901 ttcatgccag ctgcgatgnc ccgcagcatg cccaccagct gcagcacgct gaactnncca
      961 tcctnnnncc gaaggaactn nnccagggcc ccattcnnnc atg
//
