LOCUS       Exported               14701 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    pINDUCER11 (miR-RUG)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14701)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 14701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14701
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    500..733
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             918..962
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1260..1377
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1442..1816)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1813..1822
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(1826..1844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1836..1883)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    1953..2223
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2256..2294
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2291..2315
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2391..3086
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3137..3230
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     3230..3246
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3231..3247
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3268..3285
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3286..3309
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      3319..3328
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4604..4624
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     ncRNA           4648..4760
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        4811..6022
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5859..5878
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(6029..6062)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             6069..6815
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6864..7436
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7031..7048)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7258..7277
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7454..8173
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7454..8170
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7499..7520)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7760..7779)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8107..8128
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8195..8783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8248..8268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8666..8677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8990..9223
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9324..9341)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9330..9554
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9600..10028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9687..9706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9897..9918
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10037..10057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10042..10371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10222..10357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10284..10303
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11194..11315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11364..11380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11364..11380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11377..11399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11388..11404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11412..11442)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11457..11478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11595..11612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11766..12354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11846..11865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12525..13385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13148..13167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13386..13490)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13538..13672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             13840..14473
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    14520..14645
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
       61 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      121 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      181 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
      241 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
      301 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
      361 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
      421 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
      481 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
      541 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
      601 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
      661 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
      721 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
      781 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
      841 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
      901 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
      961 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1021 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1081 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     1141 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     1201 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     1261 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1321 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     1381 gtttattaca gggacagcag agatccagtt tggttagtac cgggcccgct ctagtccgga
     1441 atcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
     1501 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
     1561 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
     1621 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
     1681 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
     1741 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
     1801 tggtcaactt ggccatggtg gccctcctat agtgagtcgt attatactat gccgatatac
     1861 tatgccgatg attaattgtc aacacgtgct gcaggtccga ggttctaggc tagcttaaag
     1921 gaaccaattc agtcgactgg atcctttacc actccctatc agtgatagag aaaagtgaaa
     1981 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2041 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2101 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2161 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2221 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2281 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     2341 ttgacctcca tagaagacac cgggaccgat ccagcctacc ggtcgccacc atgagcgagc
     2401 tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     2461 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca
     2521 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     2581 acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     2641 tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc gtgctgaccg
     2701 ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag atcaacgggg
     2761 tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg gaggccaaca
     2821 ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg gccctgaagc
     2881 tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc aagaaacccg
     2941 ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa agaatcaagg
     3001 aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag tactgcgacc
     3061 tccctagcaa actggggcac agatgatgcg gccgcaagcc ttgttaagtg ctcgcttcgg
     3121 cagcacatat actatgttga atgaggcttc agtactttac agaatcgttg cctgcacatc
     3181 ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct cgaggtcgac
     3241 ggtatcgata agcttgacgt ccatatgctg gtaccccgtg gaagtctgga agttctcttc
     3301 caggggcccg aaggagacgc caccatgggt ctagataggg ataacagggt aatggtaccg
     3361 gatcgatccc ccaacttcca ggcccgccat agtgatttga tttgccagcg catcgctatc
     3421 aatcgccggg ttcacccatt cgcgtaacca cagttcactg aatttcataa tctattcctg
     3481 ccttatttaa actgtttgag gaaacgcaga tcgttttcga agaatgaacg caggtcggtg
     3541 acgccgtaac gcaacatagt cagacgctcc atccccatcc cgaagccgaa accagagtaa
     3601 acttccgggt cgatgccaac gttacgcaac acgttcggat gcaccatccc gcagcccagc
     3661 acttccagcc atttaccgtt tttacccatg acgtccactt ctgcagaagg ttcggtaaac
     3721 gggaagtagg aaggacggaa gcgaatctgc aaatcttcct caaagaagtt acgcaggaag
     3781 tcgtgcagcg tgcctttcag gttggtaaag ctgatgttgg tatcaacaat cagaccttcc
     3841 atctgatgga acatcggcgt gtgagtctgg tcgtagtcgt tacgataaac acggccaggc
     3901 gcgatgatac gaatcggtgg ctgctgggct ttcatggtgc ggatctgtac gccagaggtc
     3961 tgggtacgca gcaggcgggt agtgtcaaac cagaaagtgt cgtggtcagc gcgcgccggg
     4021 tggtgaccag gaatgttcag agcatcgaag ttatgataat cgtcttcgat ttccggcccg
     4081 gttgccacgg taaagccaag ctcaccgaag aaactttcga tacggtcgat ggtacgggta
     4141 accggatgca gaccgccgtt ttcaatgcga cgacctggca gagagacatc aatcgtttcc
     4201 gccgccagac gcgcattcag tgcagcgctt tccagttccg ctttacgcgc attcagcgcc
     4261 tgctgaacct gctctttcgc ttcgttgata accgcaccag ctgccggacg ctcttctggc
     4321 ggcagctcac gcagggtcgt catctgaagg gttaagtgcc cttttttacc caaatattcg
     4381 acgcgcacat tatctaacgc ggcaacatct gacgcctggc taatggccgc cttcgcactg
     4441 gcaaccagtt ctgcgagatg tgacatggtt ttcctcattg tgtcagtggt gacactggtt
     4501 cgttggactt agagcctatc ccatcaggct attttacttg ccattttggt ccccgatacc
     4561 gtcgaccgcg gattaccctg ttatccctag atctcacaat tctgagaatt tgtattttca
     4621 gggtgatctc cgtggatcta ttacgaattc aaggggctac tttaggagca attatcttgt
     4681 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
     4741 ggtataaatt aaatcacttt tttcaattgg aagactaatg cgtttaaaca cgcggcgacg
     4801 cgtaagatct ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg
     4861 gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg
     4921 ctcaggacag cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag
     4981 gacattttag gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga
     5041 acaggcgagg aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt
     5101 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     5161 ggatttgggt cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg
     5221 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
     5281 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
     5341 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
     5401 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5461 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5521 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
     5581 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
     5641 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5701 ggtaggtgtg tggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5761 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5821 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     5881 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     5941 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     6001 gtttttggct tttttgttag acggatccat aacttcgtat aatgtatgct atacgaagtt
     6061 atgccaccat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     6121 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     6181 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     6241 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     6301 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     6361 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     6421 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     6481 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     6541 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     6601 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     6661 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     6721 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     6781 ttgacgattt tgaccttgac atgctccccg ggtaaataac ttcgtatagc atacattata
     6841 cgaagtatgg cgcgccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     6901 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     6961 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7021 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7081 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7141 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7201 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7261 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7321 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7381 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     7441 gtatcgatcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     7501 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     7561 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     7621 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     7681 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     7741 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     7801 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     7861 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     7921 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     7981 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     8041 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     8101 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     8161 gctgtacaag taattaatta agcgcgtctg gaacaatcaa cctctggatt acaaaatttg
     8221 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8281 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8341 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8401 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8461 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8521 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8581 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     8641 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8701 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8761 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     8821 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8881 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     8941 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     9001 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     9061 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9121 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9181 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9241 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     9301 cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     9361 ttgccccttc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9421 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9481 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     9541 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     9601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     9661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9781 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9841 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10021 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    10081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    10141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    10201 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
    10321 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
    10381 ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc tgaactcacc
    10441 gcgacgtgac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
    10501 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
    10561 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
    10621 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
    10681 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
    10741 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
    10801 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
    10861 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
    10921 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
    10981 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
    11041 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc
    11101 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    11161 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    11221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11281 agttgtggtt tgtccaaact aatcaatgta tcttatcatg tctgtatacc gtcgacctct
    11341 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    11581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    11641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    11701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    11761 gcgtttttcc ataggctccg cccccctgat gagcatcaca aaaatcgacg ctcaagtcag
    11821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    11881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    11941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12001 cgctccaagc tgggctgtgt gcactaaccc cccgttcagc ccgaccgctg cgccttatcc
    12061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    12121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    12181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    12241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    12481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    12541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    12601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    12661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    12721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    12781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    12841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    12901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    13021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    13141 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    13201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    13261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    13501 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
    13561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    13621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    13681 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    13741 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    13801 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    13861 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    13921 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
    13981 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
    14041 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
    14101 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    14161 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
    14221 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
    14281 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    14341 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    14401 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    14461 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    14521 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    14581 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    14641 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttarggagag
    14701 c
//
