LOCUS Exported 13088 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS LRRK2-WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13088) AUTHORS West AB, Moore DJ, Biskup S, Bugayenko A, Smith WW, Ross CA, Dawson VL, Dawson TM TITLE Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity. JOURNAL Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16842-7. Epub 2005 Nov 3. PUBMED 16269541 REFERENCE 2 (bases 1 to 13088) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13088 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 984..993 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8592..8621 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 8637..8654 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(8677..8694) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8683..8907 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8953..9381 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9040..9059) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9250..9271 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9390..9410) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9395..9724 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9575..9710 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9637..9656 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9791..10585 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9845..9864) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10455..10474 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 10761..10882 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10798..10817) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10852..10871 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10931..10947) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10931..10947) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10944..10966) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10955..10971 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10979..11009) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11024..11045 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(11162..11179) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11333..11921) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11413..11432) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12092..12952) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12715..12734 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12953..13057) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatcctgccg gttccctgag cagcggacgt tcatgctggg 961 agggcggcgg gttggaagca ggtgccacca tggctagtgg cagctgtcag gggtgcgaag 1021 aggacgagga aactctgaag aagttgatag tcaggctgaa caatgtccag gaaggaaaac 1081 agatagaaac gctggtccaa atcctggagg atctgctggt gttcacgtac tccgagcacg 1141 cctccaagtt atttcaaggc aaaaatatcc atgtgcctct gttgatcgtc ttggactcct 1201 atatgagagt cgcgagtgtg cagcaggtgg gttggtcact tctgtgcaaa ttaatagaag 1261 tctgtccagg tacaatgcaa agcttaatgg gaccccagga tgttggaaat gattgggaag 1321 tccttggtgt tcaccaattg attcttaaaa tgctaacagt tcataatgcc agtgtaaact 1381 tgtcagtgat tggactgaag accttagatc tcctcctaac ttcaggtaaa atcaccttgc 1441 tgatactgga tgaagaaagt gatattttca tgttaatttt tgatgccatg cactcatttc 1501 cagccaatga tgaagtccag aaacttggat gcaaagcttt acatgtgctg tttgagagag 1561 tctcagagga gcaactgact gaatttgttg agaacaaaga ttatatgata ttgttaagtg 1621 cgttaacaaa ttttaaagat gaagaggaaa ttgtgcttca tgtgctgcat tgtttacatt 1681 ccctagcgat tccttgcaat aatgtggaag tcctcatgag tggcaatgtc aggtgttata 1741 atattgtggt ggaagctatg aaagcattcc ctatgagtga aagaattcaa gaagtgagtt 1801 gctgtttgct ccataggctt acattaggta attttttcaa tatcctggta ttaaacgaag 1861 tccatgagtt tgtggtgaaa gctgtgcagc agtacccaga gaatgcagca ttgcagatct 1921 cagcgctcag ctgtttggcc ctcctcactg agactatttt cttaaatcaa gatttagagg 1981 aaaagaatga gaatcaagag aatgatgatg agggggaaga agataaattg ttttggctgg 2041 aagcctgtta caaagcatta acgtggcata gaaagaacaa gcacgtgcag gaggccgcat 2101 gctgggcact aaataatctc cttatgtacc aaaacagttt acatgagaag attggagatg 2161 aagatggcca tttcccagct catagggaag tgatgctctc catgctgatg cattcttcat 2221 caaaggaagt tttccaggca tctgcgaatg cattgtcaac tctcttagaa caaaatgtta 2281 atttcagaaa aatactgtta tcaaaaggaa tacacctgaa tgttttggag ttaatgcaga 2341 agcatataca ttctcctgaa gtggctgaaa gtggctgtaa aatgctaaat catctttttg 2401 aaggaagcaa cacttccctg gatataatgg cagcagtggt ccccaaaata ctaacagtta 2461 tgaaacgtca tgagacatca ttaccagtgc agctggaggc gcttcgagct attttacatt 2521 ttatagtgcc tggcatgcca gaagaatcca gggaggatac agaatttcat cataagctaa 2581 atatggttaa aaaacagtgt ttcaagaatg atattcacaa actggtccta gcagctttga 2641 acaggttcat tggaaatcct gggattcaga aatgtggatt aaaagtaatt tcttctattg 2701 tacattttcc tgatgcatta gagatgttat ccctggaagg tgctatggat tcagtgcttc 2761 acacactgca gatgtatcca gatgaccaag aaattcagtg tctgggttta agtcttatag 2821 gatacttgat tacaaagaag aatgtgttca taggaactgg acatctgctg gcaaaaattc 2881 tggtttccag cttataccga tttaaggatg ttgctgaaat acagactaaa ggatttcaga 2941 caatcttagc aatcctcaaa ttgtcagcat ctttttctaa gctgctggtg catcattcat 3001 ttgacttagt aatattccat caaatgtctt ccaatatcat ggaacaaaag gatcaacagt 3061 ttctaaacct ctgttgcaag tgttttgcaa aagtagctat ggatgattac ttaaaaaatg 3121 tgatgctaga gagagcgtgt gatcagaata acagcatcat ggttgaatgc ttgcttctat 3181 tgggagcaga tgccaatcaa gcaaaggagg gatcttcttt aatttgtcag gtatgtgaga 3241 aagagagcag tcccaaattg gtggaactct tactgaatag tggatctcgt gaacaagatg 3301 tacgaaaagc gttgacgata agcattggga aaggtgacag ccagatcatc agcttgctct 3361 taaggaggct ggccctggat gtggccaaca atagcatttg ccttggagga ttttgtatag 3421 gaaaagttga accttcttgg cttggtcctt tatttccaga taagacttct aatttaagga 3481 aacaaacaaa tatagcatct acactagcaa gaatggtgat cagatatcag atgaaaagtg 3541 ctgtggaaga aggaacagcc tcaggcagcg atggaaattt ttctgaagat gtgctgtcta 3601 aatttgatga atggaccttt attcctgact cttctatgga cagtgtgttt gctcaaagtg 3661 atgacctgga tagtgaagga agtgaaggct catttcttgt gaaaaagaaa tctaattcaa 3721 ttagtgtagg agaattttac cgagatgccg tattacagcg ttgctcacca aatttgcaaa 3781 gacattccaa ttccttgggg cccatttttg atcatgaaga tttactgaag cgaaaaagaa 3841 aaatattatc ttcagatgat tcactcaggt catcaaaact tcaatcccat atgaggcatt 3901 cagacagcat ttcttctctg gcttctgaga gagaatatat tacatcacta gacctttcag 3961 caaatgaact aagagatatt gatgccctaa gccagaaatg ctgtataagt gttcatttgg 4021 agcatcttga aaagctggag cttcaccaga atgcactcac gagctttcca caacagctat 4081 gtgaaactct gaagagtttg acacatttgg acttgcacag taataaattt acatcatttc 4141 cttcttattt gttgaaaatg agttgtattg ctaatcttga tgtctctcga aatgacattg 4201 gaccctcagt ggttttagat cctacagtga aatgtccaac tctgaaacag tttaacctgt 4261 catataacca gctgtctttt gtacctgaga acctcactga tgtggtagag aaactggagc 4321 agctcatttt agaaggaaat aaaatatcag ggatatgctc ccccttgaga ctgaaggaac 4381 tgaagatttt aaaccttagt aagaaccaca tttcatccct atcagagaac tttcttgagg 4441 cttgtcctaa agtggagagt ttcagtgcca gaatgaattt tcttgctgct atgcctttct 4501 tgcctccttc tatgacaatc ctaaaattat ctcagaacaa attttcctgt attccagaag 4561 caattttaaa tcttccacac ttgcggtctt tagatatgag cagcaatgat attcagtacc 4621 taccaggtcc cgcacactgg aaatctttga acttaaggga actcttattt agccataatc 4681 agatcagcat cttggacttg agtgaaaaag catatttatg gtctagagta gagaaactgc 4741 atctttctca caataaactg aaagagattc ctcctgagat tggctgtctt gaaaatctga 4801 catctctgga tgtcagttac aacttggaac taagatcctt tcccaatgaa atggggaaat 4861 taagcaaaat atgggatctt cctttggatg aactgcatct taactttgat tttaaacata 4921 taggatgtaa agccaaagac atcataaggt ttcttcaaca gcgattaaaa aaggctgtgc 4981 cttataaccg aatgaaactt atgattgtgg gaaatactgg gagtggtaaa accaccttat 5041 tgcagcaatt aatgaaaacc aagaaatcag atcttggaat gcaaagtgcc acagttggca 5101 tagatgtgaa agactggcct atccaaataa gagacaaaag aaagagagat ctcgtcctaa 5161 atgtgtggga ttttgcaggt cgtgaggaat tctatagtac tcatccccat tttatgacgc 5221 agcgagcatt gtaccttgct gtctatgacc tcagcaaagg acaggctgaa gttgatgcca 5281 tgaagccttg gctcttcaat ataaaggctc gcgcttcttc ttcccctgtg atcctcgttg 5341 gcacacattt ggatgtttct gatgagaagc aacgcaaagc ctgcatgagt aaaatcacca 5401 aggaactcct gaataagcga gggttccctg ccatacgaga ttaccacttt gtgaatgcca 5461 ccgaggaatc tgatgctttg gcaaaacttc ggaaaaccat cataaacgag agccttaatt 5521 tcaagatccg agatcagctt gttgttggac agctgattcc agactgctat gtagaacttg 5581 aaaaaatcat tttatcggag cgtaaaaatg tgccaattga atttcccgta attgaccgga 5641 aacgattatt acaactagtg agagaaaatc agctgcagtt agatgaaaat gagcttcctc 5701 acgcagttca ctttctaaat gaatcaggag tccttcttca ttttcaagac ccagcactgc 5761 agttaagtga cttgtacttt gtggaaccca agtggctttg taaaatcatg gcacagattt 5821 tgacagtgaa agtggaaggt tgtccaaaac accctaaggg aattatttcg cgtagagatg 5881 tggaaaaatt tctttcaaag aaaaggaaat ttccaaagaa ctacatgtca cagtatttta 5941 agctcctaga aaaattccag attgctttgc caataggaga agaatatttg ctggttccaa 6001 gcagtttgtc tgaccacagg cctgtgatag agcttcccca ttgtgagaac tctgaaatta 6061 tcatccgact atatgaaatg ccttattttc caatgggatt ttggtcaaga ttaatcaatc 6121 gattacttga gatttcacct tacatgcttt cagggagaga acgagcactt cgcccaaaca 6181 gaatgtattg gcgacaaggc atttacttaa attggtctcc tgaagcttat tgtctggtag 6241 gatctgaagt cttagacaat catccagaga gtttcttaaa aattacagtt ccttcttgta 6301 gaaaaggctg tattcttttg ggccaagttg tggaccacat tgattctctc atggaagaat 6361 ggtttcctgg gttgctggag attgatattt gtggtgaagg agaaactctg ttgaagaaat 6421 gggcattata tagttttaat gatggcgaag aacatcaaaa aatcttactt gatgacttga 6481 tgaagaaagc agaggaagga gatctcttag taaatccaga tcaaccaagg ctcaccattc 6541 caatatctca gattgcccct gacttgattt tggctgacct gcctagaaat attatgttga 6601 ataatgatga gttggaattt gaacaagctc cagagtttct cctaggtgat ggcagttttg 6661 gatcagttta ccgagcagcc tatgaaggag aagaagtggc tgtgaagatt tttaataaac 6721 atacatcact caggctgtta agacaagagc ttgtggtgct ttgccacctc caccacccca 6781 gtttgatatc tttgctggca gctgggattc gtccccggat gttggtgatg gagttagcct 6841 ccaagggttc cttggatcgc ctgcttcagc aggacaaagc cagcctcact agaaccctac 6901 agcacaggat tgcactccac gtagctgatg gtttgagata cctccactca gccatgatta 6961 tataccgaga cctgaaaccc cacaatgtgc tgcttttcac actgtatccc aatgctgcca 7021 tcattgcaaa gattgctgac tacggcattg ctcagtactg ctgtagaatg gggataaaaa 7081 catcagaggg cacaccaggg tttcgtgcac ctgaagttgc cagaggaaat gtcatttata 7141 accaacaggc tgatgtttat tcatttggtt tactactcta tgacattttg acaactggag 7201 gtagaatagt agagggtttg aagtttccaa atgagtttga tgaattagaa atacaaggaa 7261 aattacctga tccagttaaa gaatatggtt gtgccccatg gcctatggtt gagaaattaa 7321 ttaaacagtg tttgaaagaa aatcctcaag aaaggcctac ttctgcccag gtctttgaca 7381 ttttgaattc agctgaatta gtctgtctga cgagacgcat tttattacct aaaaacgtaa 7441 ttgttgaatg catggttgct acacatcaca acagcaggaa tgcaagcatt tggctgggct 7501 gtgggcacac cgacagagga cagctctcat ttcttgactt aaatactgaa ggatacactt 7561 ctgaggaagt tgctgatagt agaatattgt gcttagcctt ggtgcatctt cctgttgaaa 7621 aggaaagctg gattgtgtct gggacacagt ctggtactct cctggtcatc aataccgaag 7681 atgggaaaaa gagacatacc ctagaaaaga tgactgattc tgtcacttgt ttgtattgca 7741 attccttttc caagcaaagc aaacaaaaaa attttctttt ggttggaacc gctgatggca 7801 agttagcaat ttttgaagat aagactgtta agcttaaagg agctgctcct ttgaagatac 7861 taaatatagg aaatgtcagt actccattga tgtgtttgag tgaatccaca aattcaacgg 7921 aaagaaatgt aatgtgggga ggatgtggca caaagatttt ctccttttct aatgatttca 7981 ccattcagaa actcattgag acaagaacaa gccaactgtt ttcttatgca gctttcagtg 8041 attccaacat cataacagtg gtggtagaca ctgctctcta tattgctaag caaaatagcc 8101 ctgttgtgga agtgtgggat aagaaaactg aaaaactctg tggactaata gactgcgtgc 8161 actttttaag ggaggtaacg gtaaaagaaa acaaggaatc aaaacacaaa atgtcttatt 8221 ctgggagagt gaaaaccctc tgccttcaga agaacactgc tctttggata ggaactggag 8281 gaggccatat tttactcctg gatctttcaa ctcgtcgact tatacgtgta atttacaact 8341 tttgtaattc ggtcagagtc atgatgacag cacagctagg aagccttaaa aatgtcatgc 8401 tggtattggg ctacaaccgg aaaaatactg aaggtacaca aaagcagaaa gagatacaat 8461 cttgcttgac cgtttgggac atcaatcttc cacatgaagt gcaaaattta gaaaaacaca 8521 ttgaagtgag aaaagaatta gctgaaaaaa tgagacgaac atctgttgag ctcgagtcta 8581 gagggccctt cgaacaaaaa ctcatctcag aagaggatct gaatatgcat accggtcatc 8641 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc 8701 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 8761 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 8821 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 8881 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 8941 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 9001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 9061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 9121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 9181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 9241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 9301 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 9361 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 9421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 9481 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 9541 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 9601 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 9661 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 9721 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg 9781 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 9841 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 9901 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 9961 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 10021 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 10081 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 10141 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 10201 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 10261 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 10321 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 10381 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 10441 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 10501 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 10561 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag 10621 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 10681 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 10741 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 10801 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 10861 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 10921 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 10981 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 11041 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 11101 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 11161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 11221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 11281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 11341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 11401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 11461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 11521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 11581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 11641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 11701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 11761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 11821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 11881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 11941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 12001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 12061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 12121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 12181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 12241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 12301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 12361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 12421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 12481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 12541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 12601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 12661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 12721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 12781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 12841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 12901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 12961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 13021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 13081 ctgacgtc //