LOCUS       Exported                9479 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2.
ACCESSION   .
VERSION     .
KEYWORDS    pBDKRB2_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2177..2197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2198..2218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2237..2263
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2291..4789
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             4793..4813
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4832..4981
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5009..5062
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5078..5464
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5519..5539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5555..6097
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             6122..6493
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(6521..6538)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6527..6734
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6743..6883
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6743..6872
                     /label=AAV2 ITR
     rep_origin      6958..7413
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7045..7064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7255..7276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7430..7449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7549..7571
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7609..7627)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7695..7799
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7800..8660
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8018..8037)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8831..9419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9320..9339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgcgcc accatgaaga cgatcatcgc
      841 cctgagctac atcttctgcc tggtattcgc cgactacaag gacgatgatg acgccagcat
      901 cgatatgttc tcaccctgga agatttctat gttcctttct gtgcgcgagg acagcgtgcc
      961 cacaactgcg agttttagcg ccgacatgct taacgtgacc ctgcagggcc ccactctcaa
     1021 cgggacattt gctcagtcta aatgtcctca agtggaatgg ctcggctggt tgaacactat
     1081 ccagccccct ttcctttggg ttctttttgt tctggccacg ctggaaaaca tctttgttct
     1141 ctctgtgttt tgcctccata agagctcctg tacggtggcc gaaatttacc tgggtaattt
     1201 ggccgcagcg gatctcattt tggcctgtgg cctgcctttc tgggctatta ctatcagcaa
     1261 taattttgat tggttgtttg gcgagacctt gtgtcgcgtg gtgaatgcaa ttatcagtat
     1321 gaatctgtac agctcaatct gcttccttat gctcgtcagc attgacaggt acctcgccct
     1381 tgtaaagact atgagcatgg gccgcatgag gggcgtacgc tgggctaagc tctatagcct
     1441 ggtgatttgg ggttgcaccc tcctcctcag ttcacctatg ctggtcttcc gaacgatgaa
     1501 ggagtactct gacgagggcc acaatgttac cgcatgcgtt atcagttacc catctttgat
     1561 ttgggaggtc ttcacaaaca tgctgctgaa cgtggtgggt tttttgctcc ccctttctgt
     1621 gattaccttc tgtacaatgc agatcatgca agttttgcga aacaacgaaa tgcaaaaatt
     1681 taaggagata cagaccgagc gaagggccac agtgctggtc ttggtcgttt tgctgttgtt
     1741 cattatctgt tggctgcctt tccagatcag cactttcctg gacacgttgc acagactcgg
     1801 aatcctgagt agttgtcagg acgaaaggat catcgacgtt atcactcaaa tcgcgtcctt
     1861 catggcatac tccaatagct gcttgaaccc attggtgtat gttattgtgg gaaaacggtt
     1921 taggaagaag agctgggaag tgtaccaagg ggtgtgccaa aagggaggct gccgatcaga
     1981 gccaatccaa atggaaaata gcatgggtac cctgagaacc tccattagcg tggagcgaca
     2041 gatccacaag ctccaggact gggctggctc tagacagatc gataccggtg gacgcacccc
     2101 acccagcctg ggtccccaag atgagtcctg caccaccgcc agctcctccc tggccaagga
     2161 cacttcatcg accggtgaga acctgtactt ccagggcccc aagaagaaga ggaaggtggg
     2221 gggaggcagc actagttacc catacgacgt accagattac gctggaggtt ctggatccgg
     2281 cggtggctcg aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct
     2341 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat
     2401 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg
     2461 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa
     2521 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat
     2581 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa
     2641 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga
     2701 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa
     2761 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca
     2821 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc
     2881 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg
     2941 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa
     3001 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg
     3061 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta
     3121 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg
     3181 gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat
     3241 cgacaacaag gtgctgacca gaagcgacaa ggcccggggc aagagcgaca acgtgccctc
     3301 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat
     3361 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga
     3421 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc
     3481 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga
     3541 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt
     3601 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     3661 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga
     3721 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc
     3781 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct
     3841 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat
     3901 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt
     3961 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc
     4021 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg
     4081 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg
     4141 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag
     4201 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa
     4261 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag
     4321 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata
     4381 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa
     4441 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca
     4501 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc
     4561 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct
     4621 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga
     4681 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat
     4741 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca gccccaagaa
     4801 gaagagaaag gtggaggcca gcggacgggc tgacgcattg gacgattttg atctggatat
     4861 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     4921 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     4981 gattaacggt accgctagcg gcagtggaga gggcagagga agtctgctaa catgcggtga
     5041 cgtcgaggag aatcctggcc cagtgagcaa gcttatggct tcaaacttta ctcagttcgt
     5101 gctcgtggac aatggtggga caggggatgt gacagtggct ccttctaatt tcgctaatgg
     5161 ggtggcagag tggatcagct ccaactcacg gagccaggcc tacaaggtga catgcagcgt
     5221 caggcagtct agtgcccaga agagaaagta taccatcaag gtggaggtcc ccaaagtggc
     5281 tacccagaca gtgggcggag tcgaactgcc tgtcgccgct tggaggtcct acctgaacat
     5341 ggagctcact atcccaattt tcgctaccaa ttctgactgt gaactcatcg tgaaggcaat
     5401 gcaggggctc ctcaaagacg gtaatcctat cccttccgcc atcgccgcta actcaggtat
     5461 ctacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag gtagcggacc
     5521 taagaaaaag aggaaggtgg cggccgctgg atccccttca gggcagatca gcaaccaggc
     5581 cctggctctg gcccctagct ccgctccagt gctggcccag actatggtgc cctctagtgc
     5641 tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag gaccacccca
     5701 gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc tgagtgaagc
     5761 tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg ggaacagcac
     5821 cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc agcagctgct
     5881 gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg agtaccccga
     5941 agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc caactcccct
     6001 gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa gcatcgctga
     6061 tatggacttt agtgccctgc tgtcacagat ttcctctagt gggcagggag gaggtggaag
     6121 cggcttcagc gtggacacca gtgccctgct ggacctgttc agcccctcgg tgaccgtgcc
     6181 cgacatgagc ctgcctgacc ttgacagcag cctggccagt atccaagagc tcctgtctcc
     6241 ccaggagccc cccaggcctc ccgaggcaga gaacagcagc ccggattcag ggaagcagct
     6301 ggtgcactac acagcgcagc cgctgttcct gctggacccc ggctccgtgg acaccgggag
     6361 caacgacctg ccggtgctgt ttgagctggg agagggctcc tacttctccg aaggggacgg
     6421 cttcgccgag gaccccacca tctccctgct gacaggctcg gagcctccca aagccaagga
     6481 ccccactgtc tcctaagaat tcctagagct cgctgatcag cctcgactgt gccttctagt
     6541 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6601 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6661 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc
     6721 aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     6781 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     6841 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     6901 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     6961 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7021 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7081 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7141 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     7201 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7261 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     7321 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7381 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     7441 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7501 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7561 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7621 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7681 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7741 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7801 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7861 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7921 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7981 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8041 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8101 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8161 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8221 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8281 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8341 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8401 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8461 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc
     8521 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8581 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8641 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8701 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8761 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8821 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8881 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8941 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9001 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9061 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9121 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9181 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9241 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9301 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9361 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9421 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatg
//
