LOCUS Exported 9362 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes dCas9(C)-VP64 with SAM components fused to CXCR4. ACCESSION . VERSION . KEYWORDS pCXCR4_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9362) AUTHORS Baeumler TA, Ahmed AA, Fulga TA TITLE Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. JOURNAL Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. PUBMED 28903044 REFERENCE 2 (bases 1 to 9362) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9362 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 12..297 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 299..576 /label=chicken beta-actin promoter intron 577..804 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" CDS 2060..2080 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 2081..2101 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2120..2146 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2174..4672 /codon_start=1 /product="C-terminal portion of catalytically inactive Streptococcus pyogenes dCas9 carrying the N863A mutation (Zetsche et al., 2015)" /label=dCas9(C) /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL IHQSITGLYETRIDLSQLGGD" CDS 4676..4696 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4715..4864 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 4892..4945 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 4961..5347 /codon_start=1 /product="bacteriophage MS2 coat protein" /label=MS2-N55K /note="binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)" /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI VKAMQGLLKDGNPIPSAIAANSGIY" CDS 5402..5422 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5438..5980 /codon_start=1 /product="C-terminal portion of the p65 subunit of mouse NF-kappa-B" /label=p65 /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED FSSIADMDFSALLSQISS" CDS 6005..6376 /codon_start=1 /product="C-terminal activation domain from the human heat shock transcription factor HSF1" /label=HSF1 /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED PTISLLTGSEPPKAKDPTVS" primer_bind complement(6404..6421) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6410..6617 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6626..6766 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6626..6755 /label=AAV2 ITR rep_origin 6841..7296 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6928..6947) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7138..7159 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7313..7332) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7432..7454 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7492..7510) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7578..7682 /gene="bla" /label=AmpR promoter CDS 7683..8543 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7901..7920) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8714..9302 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9203..9222 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg 121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc 241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc 301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt 361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg 421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg 481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg 541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc 601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta 661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag 721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac 781 ctgcctgaaa tcactttttt tcaggttgga ccggtgcgcc accatgaaga cgatcatcgc 841 cctgagctac atcttctgcc tggtattcgc cgactacaag gacgatgatg acgccagcat 901 cgatatggag gggatatcaa tctacacatc agataattac acggaagaga tgggttccgg 961 cgattacgac tctatgaaag agccgtgttt tagagaggaa aacgcgaact ttaacaagat 1021 ctttctcccc accatctaca gcatcatctt tctcacaggc atcgtaggga acggcctggt 1081 catcctggtg atgggatacc aaaagaagct gaggtcaatg accgacaagt ataggctcca 1141 tctgtccgtg gccgacctcc tgtttgtgat taccctgcct ttttgggcag ttgacgctgt 1201 cgctaattgg tacttcggca acttcctctg taaggcagtg cacgttatct acactgtgaa 1261 tctttatagt tccgtcttga tcttggcctt tatcagtctc gacaggtatt tggcgattgt 1321 gcacgctacc aactcacaac gacctagaaa actcctggct gagaaggtgg tttacgttgg 1381 tgtgtggatt ccagctctcc tgttgacaat accagacttc attttcgcta atgtgagcga 1441 ggccgatgac agatacattt gtgaccgatt ttacccaaac gatctgtggg tagtggtatt 1501 tcagttccaa cacattatgg tcgggctgat cttgcccggc attgtcatac tgtcttgcta 1561 ctgcatcatt atttctaagc tgtcacactc aaaaggccac caaaagagga aggctctgaa 1621 aacaacggtg atcctgatac tggccttctt cgcatgttgg ctgccctact atatcggcat 1681 cagcattgac tcatttatac tcctggaaat tatcaagcag ggctgcgagt tcgagaacac 1741 cgttcataag tggatttcta taaccgaggc cctcgccttc tttcactgtt gtttgaatcc 1801 gattctctac gcgtttcttg gcgccaaatt taaaacaagc gcccaacatg cactgacatc 1861 agtgtctagg gggagctctc tgaaaatcct ctccaaggga aaacgaggcg gacatagcag 1921 tgtcagcact gagtccgaat ccagctcatt tcatagctct atcgataccg gtggacgcac 1981 cccacccagc ctgggtcccc aagatgagtc ctgcaccacc gccagctcct ccctggccaa 2041 ggacacttca tcgaccggtg agaacctgta cttccagggc cccaagaaga agaggaaggt 2101 ggggggaggc agcactagtt acccatacga cgtaccagat tacgctggag gttctggatc 2161 cggcggtggc tcgaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct 2221 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa 2281 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct 2341 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga 2401 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat 2461 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct 2521 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg 2581 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag 2641 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc 2701 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc 2761 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat 2821 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca 2881 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct 2941 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct 3001 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa 3061 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc 3121 catcgacaac aaggtgctga ccagaagcga caaggcccgg ggcaagagcg acaacgtgcc 3181 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct 3241 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact 3301 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt 3361 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg 3421 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca 3481 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc 3541 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg 3601 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa 3661 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac 3721 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga 3781 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca 3841 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct 3901 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta 3961 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa 4021 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag 4081 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa 4141 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa 4201 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa 4261 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga 4321 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga 4381 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct 4441 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca 4501 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat 4561 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag 4621 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acagccccaa 4681 gaagaagaga aaggtggagg ccagcggacg ggctgacgca ttggacgatt ttgatctgga 4741 tatgctggga agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct 4801 tgatgacttt gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat 4861 gctgattaac ggtaccgcta gcggcagtgg agagggcaga ggaagtctgc taacatgcgg 4921 tgacgtcgag gagaatcctg gcccagtgag caagcttatg gcttcaaact ttactcagtt 4981 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa 5041 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag 5101 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt 5161 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa 5221 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc 5281 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg 5341 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg 5401 acctaagaaa aagaggaagg tggcggccgc tggatcccct tcagggcaga tcagcaacca 5461 ggccctggct ctggccccta gctccgctcc agtgctggcc cagactatgg tgccctctag 5521 tgctatggtg cctctggccc agccacctgc tccagcccct gtgctgaccc caggaccacc 5581 ccagtcactg agcgctccag tgcccaagtc tacacaggcc ggcgagggga ctctgagtga 5641 agctctgctg cacctgcagt tcgacgctga tgaggacctg ggagctctgc tggggaacag 5701 caccgatccc ggagtgttca cagatctggc ctccgtggac aactctgagt ttcagcagct 5761 gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa ccaatgctga tggagtaccc 5821 cgaagccatt acccggctgg tgaccggcag ccagcggccc cccgaccccg ctccaactcc 5881 cctgggaacc agcggcctgc ctaatgggct gtccggagat gaagacttct caagcatcgc 5941 tgatatggac tttagtgccc tgctgtcaca gatttcctct agtgggcagg gaggaggtgg 6001 aagcggcttc agcgtggaca ccagtgccct gctggacctg ttcagcccct cggtgaccgt 6061 gcccgacatg agcctgcctg accttgacag cagcctggcc agtatccaag agctcctgtc 6121 tccccaggag ccccccaggc ctcccgaggc agagaacagc agcccggatt cagggaagca 6181 gctggtgcac tacacagcgc agccgctgtt cctgctggac cccggctccg tggacaccgg 6241 gagcaacgac ctgccggtgc tgtttgagct gggagagggc tcctacttct ccgaagggga 6301 cggcttcgcc gaggacccca ccatctccct gctgacaggc tcggagcctc ccaaagccaa 6361 ggaccccact gtctcctaag aattcctaga gctcgctgat cagcctcgac tgtgccttct 6421 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 6481 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 6541 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat 6601 agcaggcatg ctggggagcg gccgcaggaa cccctagtga tggagttggc cactccctct 6661 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 6721 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 6781 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 6901 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 7021 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 7141 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat 7201 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta 7261 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 7321 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 7381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 7441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 7501 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 7561 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 7681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 7741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 7801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 7861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 7921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 7981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 8041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 8101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 8161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 8221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 8281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 8341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggaa 8401 gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 8461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 8521 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 8581 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 8641 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 8701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 8761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 8821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 8881 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 8941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 9001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 9061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 9121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 9181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 9241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 9301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 9361 tg //