LOCUS       Exported                 435 bp ds-DNA     linear   SYN 22-JUN-2021
DEFINITION  Bacterial expression of Cysteine-modified SpyCatcher002 allowing 
            reaction with thiol coupling reagents.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST14-Cys-SpyCatcher002
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil 
            Anuar INA
  TITLE     Evolving accelerated amidation by SpyTag/SpyCatcher to analyze 
            membrane dynamics.
  JOURNAL   Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.
  PUBMED    29024296
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattgcgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt aaccacctta tcaggtttat caggtgagca aggtccgtcc
      121 ggtgatatga caactgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggccgtg agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acatgtgaag gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc gaagcaacta aaggtgacgc tcatactgga
      421 tccagtggta gctaa
//
