LOCUS Exported 489 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation. ACCESSION . VERSION . KEYWORDS pDEST14-Avitag-SpyCatcher002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 489) AUTHORS Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil Anuar INA TITLE Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. JOURNAL Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623. PUBMED 29024296 REFERENCE 2 (bases 1 to 489) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..489 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 37..81 /codon_start=1 /product="peptide tag that allows for enzymatic biotinylation" /label=AviTag(TM) /translation="GLNDIFEAQKIEWHE" CDS 106..126 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" ORIGIN 1 atgtcgtact accatcacca tcaccatcac ggcagcggcc tgaacgatat ttttgaagcg 61 cagaaaattg aatggcatga aggcgattac gacatcccaa cgaccgaaaa cctgtatttt 121 cagggcgcca tggtaaccac cttatcaggt ttatcaggtg agcaaggtcc gtccggtgat 181 atgacaactg aagaagatag tgctacccat attaaattct caaaacgtga tgaggacggc 241 cgtgagttag ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca 301 tggatttcag atggacatgt gaaggatttc tacctgtatc caggaaaata tacatttgtc 361 gaaaccgcag caccagacgg ttatgaggta gcaactgcta ttacctttac agttaatgag 421 caaggtcagg ttactgtaaa tggcgaagca actaaaggtg acgctcatac tggatccagt 481 ggtagctaa //