LOCUS Exported 9290 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes gRNA for 3' target of human ARID4B. ACCESSION . VERSION . KEYWORDS PX458_ARID4B_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9290) AUTHORS Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM TITLE CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. JOURNAL Genome Res. 2015 Sep 9. PUBMED 26355004 REFERENCE 2 (bases 1 to 9290) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9290 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 15..255 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 15..35 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 186..205 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 283..358 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 455..740 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 742..1019 /label=chicken beta-actin promoter intron 1020..1248 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 1260..1269 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1269..1334 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1341..1361 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1386..5486 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5487..5534 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5550..5603 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5604..6317 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 5604..6317 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5646..5667) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5907..5926) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6254..6275 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(6345..6362) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6351..6558 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6567..6707 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6567..6696 /label=AAV2 ITR rep_origin 6782..7237 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6869..6888) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7079..7100 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7254..7273) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7373..7395 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7433..7451) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7519..7623 /gene="bla" /label=AmpR promoter CDS 7624..8484 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7842..7861) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8655..9243 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9144..9163 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttttgctcac atgtgagggc ctatttccca tgattccttc atatttgcat atacgataca 61 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa 121 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta 181 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata 241 tcttgtggaa aggacgaaac accgttgagt gcaggtgaca gcgttttaga gctagaaata 301 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 361 ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc 421 gccaattctg cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc 481 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga 541 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 601 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 661 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 721 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct 781 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga 841 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg 901 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 961 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 1021 gagtcgctgc gacgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg 1081 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct 1141 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta 1201 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggaccggtg 1261 ccaccatgga ctataaggac cacgacggag actacaagga tcatgatatt gattacaaag 1321 acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag 1381 cagccgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg 1441 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc 1501 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg 1561 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct 1621 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca 1681 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg 1741 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa 1801 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc 1861 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg 1921 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc 1981 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca 2041 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa 2101 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg 2161 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg 2221 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca 2281 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct 2341 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc 2401 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg 2461 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc 2521 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc 2581 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc 2641 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga 2701 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca 2761 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg 2821 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata 2881 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg 2941 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc 3001 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga 3061 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa 3121 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa 3181 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg 3241 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg 3301 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg 3361 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc 3421 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg 3481 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc 3541 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga 3601 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg 3661 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga 3721 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc 3781 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc 3841 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc 3901 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa 3961 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga 4021 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc 4081 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac 4141 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga 4201 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt 4261 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca 4321 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa 4381 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga 4441 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca 4501 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc 4561 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt 4621 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg 4681 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga 4741 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg 4801 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg 4861 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg 4921 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta 4981 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac 5041 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca 5101 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg 5161 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag 5221 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata 5281 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag 5341 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca 5401 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga 5461 tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg 5521 caaaaaagaa aaaggaattc ggcagtggag agggcagagg aagtctgcta acatgcggtg 5581 acgtcgagga gaatcctggc ccagtgagca agggcgagga gctgttcacc ggggtggtgc 5641 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 5701 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 5761 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 5821 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 5881 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 5941 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 6001 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 6061 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 6121 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 6181 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 6241 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 6301 tggacgagct gtacaaggaa ttctaactag agctcgctga tcagcctcga ctgtgccttc 6361 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 6421 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 6481 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa 6541 tagcaggcat gctggggagc ggccgcagga acccctagtg atggagttgg ccactccctc 6601 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 6661 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 6721 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 6781 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 6841 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 6961 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 7021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 7081 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta 7141 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt 7201 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 7261 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 7321 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 7381 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 7441 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 7501 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 7561 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 7621 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 7681 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 7741 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 7801 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 7861 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 7921 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 7981 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 8041 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 8101 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 8161 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 8221 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 8281 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga 8341 agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 8401 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 8461 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 8521 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 8581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 8641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 8701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 8761 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 8821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 8881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 8941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 9001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 9061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 9121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 9181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 9241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc //