LOCUS Exported 19109 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.. ACCESSION . VERSION . KEYWORDS pCXLE-dCas9VPPH-T2A-GFP-shP53 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 19109) AUTHORS Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti EM, Trokovic R, Kere J, Otonkoski T TITLE Human pluripotent reprogramming with CRISPR activators. JOURNAL Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PUBMED 29980666 REFERENCE 2 (bases 1 to 19109) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..19109 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 385..661 /label=chicken beta-actin promoter intron 662..1678 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1602..1624 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1686..1705 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 1752..1778 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 1782..1802 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1812..5912 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5916..5936 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5973..6083 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" CDS 6135..6245 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" CDS 6297..6407 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" CDS 6459..6569 /codon_start=1 /product="3 tandem repeats of the minimal activation domain of herpes simplex virus VP16 (Cheng et al., 2013)" /label=VP48 /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML" CDS 6618..8468 /codon_start=1 /gene="EP300" /product="catalytic histone acetyltransferase core of the human E1A-associated protein p300 (Delvecchio et al., 2013)" /label=p300 core /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD" regulatory 8232..8241 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8505..9047 /codon_start=1 /product="C-terminal portion of the p65 subunit of mouse NF-kappa-B" /label=p65 /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED FSSIADMDFSALLSQISS" CDS 9072..9443 /codon_start=1 /product="C-terminal activation domain from the human heat shock transcription factor HSF1" /label=HSF1 /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED PTISLLTGSEPPKAKDPTVS" CDS 9477..9530 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 9537..10256 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 9537..10253 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(9582..9603) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(9843..9862) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 10190..10211 /label=EGFP-C /note="EGFP, forward primer" misc_feature 10294..10882 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(10347..10367) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10765..10776) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(10972..10991) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 11037..11092 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(11091..11110) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(11453..11469) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11453..11469) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11466..11488) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11477..11493 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11501..11531) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11546..11567 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind complement(11656..11689) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 12150..14075 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" protein_bind complement(14243..14276) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin 14463..16252 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" promoter 16723..17036 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 16955..16975 /label=mU6-F /note="Mouse U6 promoter, forward primer" primer_bind complement(17187..17204) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(17358..17946) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(17438..17457) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(18117..18977) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 18740..18759 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(18978..19082) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc 721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgaccg 1741 gtgccaccat gtacccatac gatgttccag attacgcttc gccgaagaaa aagcgcaagg 1801 tcgaagcgtc cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct 1861 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca 1921 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa 1981 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc 2041 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct 2101 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca 2161 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc 2221 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc 2281 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca 2341 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg 2401 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca 2461 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt 2521 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc 2581 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc 2641 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg 2701 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga 2761 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc 2821 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg 2881 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc 2941 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc 3001 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag 3061 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg 3121 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg 3181 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact 3241 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact 3301 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact 3361 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg 3421 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga 3481 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg 3541 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc 3601 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag 3661 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa 3721 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg 3781 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga 3841 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc 3901 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg 3961 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc 4021 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga 4081 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc 4141 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag 4201 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga 4261 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg 4321 tcgacgccat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga 4381 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga 4441 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg 4501 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca 4561 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc 4621 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc 4681 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga 4741 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga 4801 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg 4861 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct 4921 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc 4981 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc 5041 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga 5101 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata 5161 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca 5221 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga 5281 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc 5341 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc 5401 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg 5461 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc 5521 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt 5581 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca 5641 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc 5701 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc 5761 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca 5821 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga 5881 cacggatcga cctgtctcag ctgggaggcg acagccccaa gaagaagaga aaggtggagg 5941 ccagcctcga gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt gacttagaca 6001 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg 6061 acgattttga ccttgacatg ctccccgggt cactacccag cgctagcgtc gagtgcgaaa 6121 gcggcgggcc ggccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc 6181 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca 6241 tgctccccgg gtcactaccc agcgctagcg tcgagtgcga aagcggcggg ccggccgacg 6301 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg 6361 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtcactac 6421 ccagcgctag cgtcgagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact 6481 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg 6541 ctcttgacga ttttgacctt gacatgctcc ccgggtcact acccagcgct agcgtcgagt 6601 tcgaaggcag cggcgggatt ttcaaaccag aagaactacg acaggcactg atgccaactt 6661 tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct gtggaccctc 6721 agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat ctttctacca 6781 ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc gatgatattt 6841 ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta tacaaatact 6901 gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa agccttggat 6961 actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac ggcaaacagt 7021 tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat catttctgtg 7081 agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatgac ccttcccagc 7141 ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca ctggatcctg 7201 aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt gtccttcacc 7261 atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa agtgcacgaa 7321 ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt ggcacctttc 7381 tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca ggagaggtca 7441 ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc atgaaagcaa 7501 ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa gccctctttg 7561 cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt caagagtatg 7621 gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc gatagtgttc 7681 atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta attggatatt 7741 tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt ccaccaagtg 7801 agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc aagcccaagc 7861 gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt attgtccatg 7921 actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca aaggaattgc 7981 cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag gaactggaac 8041 aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca gatgtgacca 8101 agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa aataagagca 8161 gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac gacctctcac 8221 agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc cgcctcattg 8281 ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc atcccctgcg 8341 atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac ctggagttct 8401 cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg cacacgcaga 8461 gccaggacgt cgacactagc gtcgagttcg aaggcagcgg cgggccttca gggcagatca 8521 gcaaccaggc cctggctctg gcccctagct ccgctccagt gctggcccag actatggtgc 8581 cctctagtgc tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag 8641 gaccacccca gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc 8701 tgagtgaagc tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg 8761 ggaacagcac cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc 8821 agcagctgct gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg 8881 agtaccccga agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc 8941 caactcccct gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa 9001 gcatcgctga tatggacttt agtgccctgc tgtcacagat ttcctctagt gggcagggag 9061 gaggtggaag cggcttcagc gtggacacca gtgccctgct ggacctgttc agcccctcgg 9121 tgaccgtgcc cgacatgagc ctgcctgacc ttgacagcag cctggccagt atccaagagc 9181 tcctgtctcc ccaggagccc cccaggcctc ccgaggcaga gaacagcagc ccggattcag 9241 ggaagcagct ggtgcactac acagcgcagc cgctgttcct gctggacccc ggctccgtgg 9301 acaccgggag caacgacctg ccggtgctgt ttgagctggg agagggctcc tacttctccg 9361 aaggggacgg cttcgccgag gaccccacca tctccctgct gacaggctcg gagcctccca 9421 aagccaagga ccccactgtc tcctctagag tcgacactag cgtcgacggc tccggagagg 9481 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atcgatatgg 9541 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 9601 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 9661 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 9721 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 9781 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 9841 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 9901 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 9961 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 10021 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 10081 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 10141 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 10201 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaattta 10261 aagaattcaa gcttcgggga ctagtcatat gataatcaac ctctggatta caaaatttgt 10321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 10381 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 10441 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 10501 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 10561 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 10621 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 10681 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 10741 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 10801 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 10861 tccctttggg ccgcctcccc gcatcggtaa attcactcct caggtgcagg ctgcctatca 10921 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc 10981 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata 11041 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag 11101 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc 11161 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta 11221 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta 11281 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt 11341 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc 11401 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc 11461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 11521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 11581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatctcaatt ccgatcatat 11641 tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctgaag 11701 aggagtttac gtccagccaa gcttaggatc aattctcatg tttgacagct tatcatcgat 11761 aagctgatcc tcacaggccg cacccagctt ttcttccgtt gccccagtag catctctgtc 11821 tggtgacctt gaagaggaag aggaggggtc ccgagaatcc ccatccctac cgtccagcaa 11881 aaagggggac gaggaatttg aggcctggct tgaggctcag gacgcaaatc ttgaggatgt 11941 tcagcgggag ttttccgggc tgcgagtaat tggtgatgag gacgaggatg gttcggagga 12001 tggggaattt tcagacctgg atctgtctga cagcgaccat gaaggggatg agggtggggg 12061 ggctgttgga gggggcagga gtctgcactc cctgtattca ctgagcgtcg tctaataaag 12121 atgtctattg atctctttta gtgtgaatca tgtctgacga ggggccaggt acaggacctg 12181 gaaatggcct aggagagaag ggagacacat ctggaccaga aggctccggc ggcagtggac 12241 ctcaaagaag agggggtgat aaccatggac gaggacgggg aagaggacga ggacgaggag 12301 gcggaagacc aggagccccg ggcggctcag gatcagggcc aagacataga gatggtgtcc 12361 ggagacccca aaaacgtcca agttgcattg gctgcaaagg gacccacggt ggaacaggag 12421 caggagcagg agcgggaggg gcaggagcag gaggggcagg agcaggagga ggggcaggag 12481 caggaggagg ggcaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag 12541 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag 12601 gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag 12661 caggaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag 12721 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca ggaggggcag 12781 gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag 12841 gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggagca ggaggggcag 12901 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag 12961 caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag 13021 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg 13081 caggagcagg aggaggggca ggagcaggag gggcaggagc aggaggtgga ggccggggtc 13141 gaggaggcag tggaggccgg ggtcgaggag gtagtggagg ccggggtcga ggaggtagtg 13201 gaggccgccg gggtagagga cgtgaaagag ccaggggggg aagtcgtgaa agagccaggg 13261 ggagaggtcg tggacgtgga gaaaagaggc ccaggagtcc cagtagtcag tcatcatcat 13321 ccgggtctcc accgcgcagg ccccctccag gtagaaggcc atttttccac cctgtagggg 13381 aagccgatta ttttgaatac caccaagaag gtggcccaga tggtgagcct gacgtgcccc 13441 cgggagcgat agagcagggc cccgcagatg acccaggaga aggcccaagc actggacccc 13501 ggggtcaggg tgatggaggc aggcgcaaaa aaggagggtg gtttggaaag catcgtggtc 13561 aaggaggttc caacccgaaa tttgagaaca ttgcagaagg tttaagagct ctcctggcta 13621 ggagtcacgt agaaaggact accgacgaag gaacttgggt cgccggtgtg ttcgtatatg 13681 gaggtagtaa gacctccctt tacaacctaa ggcgaggaac tgcccttgct attccacaat 13741 gtcgtcttac accattgagt cgtctcccct ttggaatggc ccctggaccc ggcccacaac 13801 ctggcccgct aagggagtcc attgtctgtt atttcatggt ctttttacaa actcatatat 13861 ttgctgaggt tttgaaggat gcgattaagg accttgttat gacaaagccc gctcctacct 13921 gcaatatcag ggtgactgtg tgcagctttg acgatggagt agatttgcct ccctggtttc 13981 cacctatggt ggaaggggct gccgcggagg gtgatgacgg agatgacgga gatgaaggag 14041 gtgatggaga tgagggtgag gaagggcagg agtgatgtaa cttgttagga gacgccctca 14101 atcgtattaa aagccgtgta ttcccccgca ctaaagaata aatccccagt agacatcatg 14161 cgtgctgttg gtgtatttct ggccatctgt cttgtcacca ttttcgtcct cccaacatgg 14221 ggcaattgcc ggaaccctta atataacttc gtataatgta tgctatacga agttattagg 14281 tccctcgaag aggttcacta gcggatctca attgggcata cccatgttgt cacgtcactc 14341 agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac ctggtgagca 14401 atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat gggagcaacc 14461 agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa 14521 aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg catgaggata 14581 gcatatgcta cccggataca gattaggata gcatatacta cccagatata gattaggata 14641 gcatatgcta cccagatata gattaggata gcctatgcta cccagatata aattaggata 14701 gcatatacta cccagatata gattaggata gcatatgcta cccagatata gattaggata 14761 gcctatgcta cccagatata gattaggata gcatatgcta cccagatata gattaggata 14821 gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt aggatagcat 14881 atactaccct aatctctatt aggatagcat atgctacccg gatacagatt aggatagcat 14941 atactaccca gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct 15001 atgctaccca gatataaatt aggatagcat atactaccca gatatagatt aggatagcat 15061 atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat 15121 atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc caccgtgctc 15181 tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag gcgcaagtgt 15241 gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc ccacctactt 15301 atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt gaccgcagtg 15361 gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc aaaaccgcag 15421 ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa agagtggcca 15481 cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg ggggtgttag 15541 agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg ttacaaatag 15601 agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt aataacccca 15661 gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt gagatggaca 15721 tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat attcagaatg 15781 tttcattcct acactagtat ttattgccca aggggtttgt gagggttata ttggtgtcat 15841 agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt cacctgaaac 15901 cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt attctattag 15961 ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg cccgctcctt 16021 gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga atcctgaccc 16081 catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc ttaggaccct 16141 tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc tattgaatta 16201 gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg tttagggtta 16261 acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct tatcggtagc 16321 tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg ggcccctggg 16381 aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac ataaaggcaa 16441 tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca ctcagtgttg 16501 gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc ctttgtgttt 16561 ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac caaccaactg 16621 aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc aggtttagtt 16681 cgtccggggc ggggcatcga tcctctagag tcgacgctag cggatccgac gccgccatct 16741 ctaggcccgc gccggccccc tcgcacagac ttgtgggaga agctcggcta ctcccctgcc 16801 ccggttaatt tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga 16861 cagataatct gttcttttta atactagcta cattttacat gataggcttg gatttctata 16921 agagatacaa atactaaatt attattttaa aaaacagcac aaaaggaaac tcaccctaac 16981 tgtaaagtaa ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgtttggg 17041 ccgactccag tggtaatcta cttcaagaga gtagattacc actggagtct tttttggaat 17101 tcctgcagcc cgggggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 17161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 17221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 17281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 17341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 17401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 17461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 17521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 17581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 17641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 17701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 17761 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 17821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 17881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 17941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 18001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 18061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 18121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 18181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 18241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 18301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 18361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 18421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 18481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 18541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 18601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 18661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 18721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 18781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 18841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 18901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 18961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 19021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 19081 cgcacatttc cccgaaaagt gccacctgg //