LOCUS Exported 10857 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses mouse Fzd6 in mammalian cells. ACCESSION . VERSION . KEYWORDS pLEX307-mFzd6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10857) AUTHORS Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R, Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH TITLE Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. JOURNAL Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509. PUBMED 28885975 REFERENCE 2 (bases 1 to 10857) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10857 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1318 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter complement(3520..3537) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3542..3676 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(3766..3790) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3798..3839 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3890..4478 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3943..3963) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4361..4372) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4550..4783 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4861..4982 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 5022..5157 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5177..5196) /label=T7 /note="T7 promoter, forward primer" promoter complement(5178..5196) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5206..5223) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5206..5222) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5215..5237) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5364..5819 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5451..5470) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5661..5682 /label=F1ori-F /note="F1 origin, forward primer" promoter 5845..5949 /gene="bla" /label=AmpR promoter CDS 5950..6810 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6168..6187) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6981..7569 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7470..7489 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7723..7740 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7857..7878 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7893..7923 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7931..7947 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7936..7958 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7955..7971 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7955..7971 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7990..8010 /label=T3 /note="T3 promoter, forward primer" promoter 7992..8010 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 8038..8264 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 8265..8445 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8492..8617 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9110..9343 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9528..9572 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9803..10013 /label=SV40 promoter /note="SV40 early promoter" promoter 9825..10021 /label=SV40 promoter /note="SV40 early promoter" rep_origin 9872..10007 /label=SV40 ori /note="SV40 origin of replication" CDS 10053..10655 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(10053..10072) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 10549..10569 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 10698..10815 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg 1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt caggccggcc taccatggaa 1381 aggtccccgt ttctgttggc gtgcattctt ctgcccctcg taagaggaca cagccttttc 1441 acctgtgagc caatcaccgt tcccagatgt atgaaaatga cttacaacat gacgttcttc 1501 cctaacctga tgggtcatta tgaccagggg atcgctgctg tggaaatggg gcactttctg 1561 catcttgcaa atctagaatg ttcaccaaac attgaaatgt tcctttgcca agcttttata 1621 ccaacctgca cagagcaaat tcatgtagtt ctaccctgtc ggaaattgtg tgagaaaata 1681 gtttctgatt gcaaaaaact aatggacact tttggcatcc gatggcctga agaacttgaa 1741 tgtaacagat tgccacactg tgatgacact gttcctgtaa cttctcatcc acacacagag 1801 ctttctgggc cacagaagaa atcagatcaa gtcccaagag acattggatt ttggtgtcca 1861 aagcacctta ggacttccgg ggaccaaggc tataggtttc tgggaattga acagtgtgcc 1921 cctccgtgcc ccaatatgta ttttaaaagt gatgaactag actttgccaa aagtttcata 1981 ggaatagttt caatattttg tctttgtgca actctgttca cgttccttac atttttaatt 2041 gacgttagac gattcagata cccagagaga ccaattatct attactctgt ctgctacagc 2101 attgtctctc tcatgtactt cgtggggttt ttgctgggca atagcacggc ttgtaataag 2161 gcagacgaga agctggagct cggggacacc gttgtcctag ggtcaaagaa taaggcttgc 2221 agtgtggtat ttatgtttct gtattttttt acaatggctg gcaccgtgtg gtgggtgatt 2281 ctcaccatta cgtggttctt agctgccggg agaaaatgga gttgcgaagc tattgaacaa 2341 aaagcagtgt ggttccatgc cgttgcctgg ggggcgcccg ggttcctgac cgtcatgctg 2401 ctcgctatga ataaggttga aggagacaac attagcggcg tttgcttcgt tggcctgtat 2461 gacctggacg cctctcgcta cttcgtcctt ctgcctctgt gcctctgcgt atttgttggg 2521 ctgtctctcc tcttagccgg catcatctcc ttgaatcatg tccgacaagt catacagcat 2581 gatggccgga accaagagaa gctaaagaaa ttcatgattc gcatcggagt cttcagtggc 2641 ctgtatcttg tgcccttagt gacacttctc ggttgctatg tctatgagct agtgaacagg 2701 atcacctggg agatgacatg gttctctgat cattgtcacc agtaccgcat cccgtgccct 2761 taccaggcaa atccaaaagc tcgaccagaa ttggctttat ttatgataaa atatctgatg 2821 acattaattg ttggtatctc tgcggtcttc tgggttggaa gcaaaaagac gtgcacagaa 2881 tgggccgggt tctttaagcg aaaccgcaag cgagacccca tcagtgagag ccgccgagtg 2941 ctgcaagagt cctgtgagtt cttcctgaag cacaactcta aagtgaagca caagaagaag 3001 catggcgcac cagggcctca taggctgaag gtcatttcca agtccatggg aactagcaca 3061 ggagcgacca caaatcatgg cacctctgcc atggcaatcg ctgaccatga ttacttaggg 3121 caagaaactt caacagaagt ccacacctcc ccagaagcat ccgtcaaaga gggacgagca 3181 gaccgagcaa acactcccag cgccaaagat cgggactgtg gggaatctgc agggcccagt 3241 tccaagctct ctgggaaccg gaacggcagg gaaagccgag cgggcggcct gaaggagaga 3301 agcaatggat cagagggggc tccaagtgaa ggaagggtaa gtccaaagag cagcgttcct 3361 gagactggcc tgatagactg cagcacttca caggccgcca gttctccaga accaaccagc 3421 ctcaagggct ccacatctct gcctgttcac tcagcttcca gagctaggaa agagcagggt 3481 gctggcagcc attccgacgc ttgaggcgcg cctctagaac tatagtgagt cgtattacgt 3541 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 3601 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 3661 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 3721 ggtgtgggag gttttttaat tcgcggccgc actcgagata tctagaccca gctttcttgt 3781 acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 3841 agtaatgaac tagtaccggt taagtcgaca atcaacgcgt taagtcgaca atcaacctct 3901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 3961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 4021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 4081 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 4141 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 4201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 4261 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc 4321 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 4381 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 4441 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact 4501 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 4561 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac 4621 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 4681 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 4741 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca 4801 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 4861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 4921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 4981 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 5041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 5101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 5161 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 5221 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 5281 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 5341 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 5401 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 5461 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 5521 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 5581 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 5641 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 5701 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 5761 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 5821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 5881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 5941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 6001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 6061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 6121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 6181 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 6241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 6301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 6361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 6421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 6481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 6541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 6601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 6661 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 6721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 6781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 6841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 6961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 7021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 7081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 7141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 7201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 7261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 7321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 7381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 7441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 7501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 7561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 7621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 7681 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 7741 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 7801 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 7861 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 7921 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 7981 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt 8041 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 8101 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg 8161 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc 8221 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag 8281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 8341 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 8401 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 8461 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 8521 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 8581 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 8641 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 8701 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 8761 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 8821 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 8881 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 8941 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 9001 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 9061 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 9121 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 9181 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 9241 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 9301 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 9361 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 9421 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 9481 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 9541 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 9601 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 9661 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 9721 cccacctccc aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg 9781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc 9841 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 9901 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 9961 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 10021 aaagctttct agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc 10081 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 10141 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 10201 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 10261 gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 10321 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 10381 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 10441 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 10501 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 10561 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 10621 cctggtgcat gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg 10681 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 10741 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 10801 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccgg //