LOCUS Exported 7301 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence. ACCESSION . VERSION . KEYWORDS pcDNA3-Antares2 c-myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7301) AUTHORS Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW TITLE Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. JOURNAL Nat Methods. 2017 Sep 4. doi: 10.1038/nmeth.4400. PUBMED 28869756 REFERENCE 2 (bases 1 to 7301) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7301 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 898..907 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2794..2823 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter complement(2854..2872) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2855..2872) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2892..2909) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2898..3122 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3168..3596 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3255..3274) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3465..3486 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3605..3625) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3610..3939 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3790..3925 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3852..3871 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4006..4800 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4060..4079) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4670..4689 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4974..5095 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5011..5030) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5065..5084 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5144..5160) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5144..5160) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5157..5179) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5168..5184 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5192..5222) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5237..5258 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5375..5392) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5546..6134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5626..5645) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6305..7165) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6928..6947 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7166..7270) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccgcc 901 accatggtga gcaagggcga ggagctgatc aaggagaaca tgagaagcaa gctgtacctg 961 gaaggcagcg tgaacggcca ccagttcaag tgcacccacg aaggggaggg caagccctac 1021 gagggcaagc agaccaacag gatcaaggtg gtggagggag gccccctgcc gttcgcattc 1081 gacatcctgg ccacccactt tatgtacggg agcaaggtgt tcatcaagta ccccgccgac 1141 ctccccgatt attttaagca gtccttccct gagggcttca catgggagag agtcatggtg 1201 ttcgaagacg ggggcgtgct gaccgccacc caggacacca gcctccagga cggcgagctc 1261 atctacaacg tcaaggtcag aggggtgaac ttcccagcca acggccccgt gatgcagaag 1321 aaaacactgg gctgggagcc cagcaccgag accatgtacc ccgctgacgg cggcctggaa 1381 ggcagatgcg acaaggccct gaagctcgtg ggcgggggcc acctgcacgt caacttcaag 1441 accacataca agtccaagaa acccgtgaag atgcccggcg tccactacgt ggaccgcaga 1501 ctggaaagaa tcaaggaggc cgacaacgag acctacgtcg agcagtacga gcacgctgtg 1561 gccagatact ccaacctggg cggaggcttc acactcgaag atttcgttgg ggactggcga 1621 cagacagccg gctacaactt gagtcaagtc cttgaacagg gaggtgtgtc cagtttgttt 1681 cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg 1741 ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc 1801 cagatcgaaa aaatttttaa ggtggtgtac cctgtggata atcatcactt taaggtgatc 1861 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga 1921 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg 1981 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc 2041 cgagtaacca tcaacggagt gaccggctgg cgtctgcatg aacgcattct ggcgaggcac 2101 gagctgatca aggagaacat gagaagcaag ctgtacctgg aaggcagcgt gaacggccac 2161 cagttcaagt gcacccacga aggggagggc aagccctacg agggcaagca gaccaacagg 2221 atcaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc cacccacttt 2281 atgtacggga gcaaggtgtt catcaagtac cccgccgacc tccccgatta ttttaagcag 2341 tccttccctg agggcttcac atgggagaga gtcatggtgt tcgaagacgg gggcgtgctg 2401 accgccaccc aggacaccag cctccaggac ggcgagctca tctacaacgt caaggtcaga 2461 ggggtgaact tcccagccaa cggccccgtg atgcagaaga aaacactggg ctgggagccc 2521 agcaccgaga ccatgtaccc cgctgacggc ggcctggaag gcagatgcga caaggccctg 2581 aagctcgtgg gcgggggcca cctgcacgtc aacttcaaga ccacatacaa gtccaagaaa 2641 cccgtgaaga tgcccggcgt ccactacgtg gaccgcagac tggaaagaat caaggaggcc 2701 gacaacgaga cctacgtcga gcagtacgag cacgctgtgg ccagatactc caacctgggc 2761 ggaggcatgg acgagctgta caagggctcc ggcgaacaaa aactcatctc agaagaggat 2821 ctgtaactcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc 2881 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 2941 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 3001 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 3061 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 3121 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 3181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 3241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 3301 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 3361 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 3421 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 3481 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 3541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 3601 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 3661 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 3721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 3781 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 3841 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 3901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 3961 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4021 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4081 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4141 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 4201 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4261 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4321 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4381 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4441 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4501 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 4561 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4621 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4681 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4741 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4801 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4861 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4921 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 4981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5101 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 5161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 5221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 5281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 5401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 5461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 5521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 5581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 5641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 5701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 5761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 6001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 6241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 6301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 6361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 6421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 6481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 7261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //