LOCUS Exported 7225 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-FLEX-ArchT-tdTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7225) AUTHORS Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES TITLE A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. JOURNAL Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. PUBMED 21811444 REFERENCE 2 (bases 1 to 7225) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7225 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 135..412 /label=chicken beta-actin promoter primer_bind 691..713 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 775..794 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind complement(829..862) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind complement(925..958) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(981..2408) /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER SEGRHHLFLYGMDELYK" regulatory 3158..3167 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind 3184..3217 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 3288..3321 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 3363..3951 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3416..3436) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3834..3845) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3994..4115 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4005..4024) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4059..4078 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 4277..4406 /label=AAV2 ITR primer_bind complement(4472..4489) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4643..5231) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4723..4742) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5402..6262) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6025..6044 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6263..6367) /gene="bla" /label=AmpR promoter rep_origin complement(6393..6848) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6530..6551) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6742..6761 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 6920..7049 /label=AAV2 ITR ORIGIN 1 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 61 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 121 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 181 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 241 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgrggggc ggggcggggc 301 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 361 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc 421 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 481 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 541 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 601 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg 661 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct 721 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg 781 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccccat aacttcgtat 841 aaagtatcct atacgaagtt atatcaaaat aggaagacca atgcttcacc atcgacccga 901 attgccaagc atcaccatcg acccataact tcgtataatg tatgctatac gaagttatac 961 tagggatccc ccctcgagtt ttacttgtac agctcgtcca tgccgtacag gaacaggtgg 1021 tggcggccct cggagcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg 1081 atgtccagct tggtgtccac gtagtagtag ccgggcagtt gcacgggctt cttggccatg 1141 tagatggtct tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggcctggtgg 1201 atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc 1261 atggtcttct tctgcattac ggggccgtcg ggggggaagt tggtgccgcg catcttcacc 1321 ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg tcacggtcac cagaccgccg 1381 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtaa 1441 tcggggatgt cggcggggtg cttcacgtac gccttggagc cgtacatgaa ctggggggac 1501 aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg 1561 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg 1621 gagccctcca tgcgcacctt gaagcgcatg aactctttga tgacggccat gttgttgtcc 1681 tcggaggagg cggtgccgga gctgccgctg ccggtgctgc cggtgccatg ccccaggaac 1741 aggtggtggc ggccctcgga gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg 1801 gaggtgatgt ccagcttggt gtccacgtag tagtagccgg gcagttgcac gggcttcttg 1861 gccatgtaga tggtcttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc 1921 tggtggatct cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc 1981 cagcccatgg tcttcttctg cattacgggg ccgtcggggg ggaagttggt gccgcgcatc 2041 ttcaccttgt agatcagcgt gccgtcctgc agggaggagt cctgggtcac ggtcaccaga 2101 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc 2161 ttgtaatcgg ggatgtcggc ggggtgcttc acgtacgcct tggagccgta catgaactgg 2221 ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg 2281 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg 2341 ttcatggagc cctccatgcg caccttgaag cgcatgaact ctttgatgac ctcctcgccc 2401 ttgctcacta ccggtcgggg ctcgggggcc tcggtgtcgc ccaggatggc cctgctcctc 2461 agcaggatga agccgaagcc cactttggcg gtcacgtcca gcaccatgaa cagcagggtc 2521 tcgattccca gtcccaccac tccagctccc tcggttccga tgatccacag gatggggtag 2581 gcggtccaca gcaccagcac cagggcggtc agggtgttga aggtgctagc cacctctggt 2641 cccctctcct tagcagcagc cctcaggctg gtagccagga agtacagcac cacgatcatg 2701 cagatggtgc tgaacagcca ccagctgtac ctagccagtg gggtgtggct cagggcgccg 2761 atcaggccgg tcacgatcat cagggcgtcc actcccacca gggttccgat gctcaccctg 2821 tccaccttag ccagcagggc caggtcgagc agaagcaaag gggtggtgaa cagccagtcg 2881 gcgtatctgg cgtagtagat gtccagcacc tcgccggcca cggtcacctc ggtcaggccg 2941 atgccgaaga acatgctcag gtaggcggcg ctggcgatgc cgggcaccag gattgtgatg 3001 ctgtagtact ccctggcctc cttgtcggtc acgccccagc ccttcacgat gaagtagaag 3061 gtgccgatca gcatcagcag ggttccgatt cccagccaca gggtctctgg ccttccgtcg 3121 cccagcaggt cgtatcctgc ctgcagagcg atggggtcca tggtggcggt accgaattct 3181 agtataactt cgtataggat actttatacg aagttatcat tgggattctt cctattttga 3241 tccaagcatc accatcgacc ctctagtcca gatctcacca tcgacccata acttcgtata 3301 gcatacatta tacgaagtta tgtccctcga agaggttcga attcgatatc aagcttatcg 3361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 3721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 3781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 3961 gtcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa 4021 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 4081 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 4141 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat 4201 gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta gcatggcggg 4261 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct 4321 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg 4381 gcctcagtga gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag 4441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 6001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 6061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 6121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6361 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa 6421 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 6481 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 6541 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 6601 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 6661 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 6721 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 6781 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 6841 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 6901 ctcttcgcta ttacgccagc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc 6961 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg 7021 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt 7081 atctacgtag ccatgctcta ggaagagtac cattgacgtc aataatgacg tatgttccca 7141 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 7201 cccacttggc agtacatcaa gtgta //