LOCUS Exported 8781 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pK-myc-Par3b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8781) AUTHORS Joberty G, Petersen C, Gao L, Macara IG TITLE The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. JOURNAL Nat Cell Biol 2000 Aug;2(8):531-9. PUBMED 10934474 REFERENCE 2 (bases 1 to 8781) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8781 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 83..104 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 119..149 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 157..173 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 162..184 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 181..197 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 181..197 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 226..605 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 606..809 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 760..780 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 806..830 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1040..1058 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1040..1057 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1071..1090 /label=pMT2-F /note="Synthetic intron, forward primer" CDS 1145..1174 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" regulatory 5293..5302 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 5306..5440 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5343..5362) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5397..5416 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 5509..5866 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5532..5552) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5717..5852 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5779..5798 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(5886..5903) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5886..5902) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5895..5917) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 6115..6570 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6202..6221) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6412..6433 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6587..6606) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6706..6728 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6766..6784) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6852..6956 /gene="bla" /label=AmpR promoter CDS 6957..7817 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7175..7194) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7988..8576 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8477..8496 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8730..8747 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 181 caggaaacag ctatgacatg attacgaatt aattcgagct cgcccgacat tgattattga 241 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 301 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 361 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 421 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 481 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 541 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 601 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 661 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 721 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 781 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac 841 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc 901 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata 961 gagtctatag gcccaccccc ttggcttcgt tagaacgcgg ctacaattaa tacataacct 1021 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct 1081 ctccacaggt gtccactccc aggtccaact gcacctcggt tctaagcttc tgcaggtcga 1141 catggaacag aaactcatct ctgaagagga tctgtctaga ggatcccggg ctagcgggcg 1201 gccgcatatg aaagtgaccg tgtgcttcgg acggacccgg gtggtcgtgc cgtgcgggga 1261 cggccacatg aaagttttca gcctcatcca gcaggcggtg acccgctacc ggaaggccat 1321 cgccaaggat ccaaactact ggatacaggt gcatcgcttg gaacatggag atggaggaat 1381 actagacctt gatgacattc tttgtgatgt agcagacgat aaagacagac tggtagcagt 1441 gtttgatgag caggatccac atcacggagg tgatggcacc agtgccagtt ccacgggtac 1501 ccagagccca gagatatttg gtagtgagct tggcaccaac aatgtctcag cctttcagcc 1561 ttaccaagca acaagtgaaa ttgaggtcac accttcagtc cttcgagcaa atatgcctct 1621 tcatgttcga cgcagtagtg acccagctct aattggcctc tccacttctg tcagtgatag 1681 taatttttcc tctgaagagc cttcaaggaa aaatcccaca cgctggtcaa caacagctgg 1741 cttcctcaag cagaacactg ctgggagtcc taaaacctgc gacaggaaga aagatgaaaa 1801 ctacagaagc ctcccgcggg atactagtaa ctggtctaac caatttcaga gagacaatgc 1861 tcgctcgtct ctgagtgcca gtcacccaat ggtgggcaag tggctggaga aacaagaaca 1921 ggatgaggat gggacagaag aggataacag tcgtgttgaa cctgttggac atgctgacac 1981 gggtttggag catataccca acttttctct ggatgatatg gtaaagctcg tagaagtccc 2041 caacgatgga gggcctctgg gaatccatgt agtgcctttc agtgctcgag gcggcagaac 2101 cctggggtta ttagtaaaac gattggagaa aggtggtaaa gctgaacatg aaaatctttt 2161 tcgtgagaat gattgcattg tcaggattaa tgatggcgac cttcgaaata gaagatttga 2221 acaagcacaa catatgtttc gccaagccat gcgtacaccc atcatttggt tccatgtggt 2281 tcctgcagca aataaagagc agtatgaaca actatcccaa agtgagaaga acaattacta 2341 ttcaagccgt tttagccctg acagccagta tattgacaac aggagtgtga acagtgcagg 2401 gcttcacacg gtgcagagag caccccgact gaaccacccg cctgagcaga tagactctca 2461 ctcaagacta cctcatagcg cacacccctc gggaaaacca ccatccgctc cagcctcggc 2521 acctcagaat gtatttagta cgactgtaag cagtggttat aacaccaaaa aaataggcaa 2581 gaggcttaat atccagctta agaaaggtac agaaggtttg ggattcagca tcacttccag 2641 agatgtaaca ataggtggct cagctccaat ctatgtgaaa aacattctcc cccggggggc 2701 ggccattcag gatggccgac ttaaggcagg agacagactt atagaggtaa atggagtaga 2761 tttagtgggc aaatcccaag aggaagttgt ttcgctgttg agaagcacca agatggaagg 2821 aactgtgagc cttctggtct ttcgccagga agacgccttc cacccaaggg aactgaatgc 2881 agagccaagc cagatgcaga ttccaaaaga aacgaaagca gaagatgagg atattgttct 2941 tacacctgat ggcaccaggg aatttctgac atttgaagtc ccacttaatg attcaggatc 3001 tgcaggcctt ggtgtcagtg tcaaaggtaa ccggtcaaaa gagaaccacg cagatttggg 3061 aatctttgtc aagtccatta ttaatggagg agcagcatct aaagatggaa ggcttcgggt 3121 gaatgatcaa ctgatagcag taaatggaga atccctgttg ggcaagacaa accaagatgc 3181 catggaaacc ctaagaaggt ctatgtctac tgaaggcaat aaacgaggaa tgatccagct 3241 tattgttgca aggagaataa gcaagtgcaa tgagctgaag tcacctggga gcccccctgg 3301 acctgagctg cccattgaaa cagcgttgga tgatagagaa cgaagaattt cccattccct 3361 ctacagtggg attgaggggc ttgatgaatc gcccagcaga aatgctgccc tcagtaggat 3421 aatgggtaaa taccagctgt cccctacagt gaatatgccc caagatgaca ctgtcattat 3481 agaagatgac aggttgccag tgcttcctcc acatctctct gaccagtcct cttccagctc 3541 ccatgatgat gtggggtttg tgacggcaga tgctggtact tgggccaagg ctgcaatcag 3601 tgattcagcc gactgctctt tgagtccaga tgttgatcca gttcttgctt ttcaacgaga 3661 aggatttgga cgtcagagta tgtcagaaaa acgcacaaag caattttcag atgccagtca 3721 attggatttc gttaaaacac gaaaatcaaa aagcatggat ttaggtatag ctgacgagac 3781 taaactcaat acagtggatg accagaaagc aggttctccc agcagagatg tgggtccttc 3841 cctgggtctg aagaagtcaa gctcgttgga gagtctgcag accgcagttg ccgaggtgac 3901 tttgaatggg gatattcctt tccatcgtcc acggccgcgg ataatcagag gcaggggatg 3961 caatgagagc ttcagagctg ccatcgacaa atcttatgat aaacccgcgg tagatgatga 4021 tgatgaaggc atggagacct tggaagaaga cacagaagaa agttcaagat cagggagaga 4081 gtctgtatcc acagccagtg atcagccttc ccactctctg gagagacaaa tgaatggaaa 4141 ccaagagaaa ggtgataaga ctgatagaaa aaaggataaa actggaaaag aaaagaagaa 4201 agatagagat aaggagaagg ataaaatgaa agccaagaag ggaatgctga agggcttggg 4261 agacatgttc aggtttggca aacatcgaaa agatgacaag attgagaaaa cgggtaaaat 4321 aaaaatacag gaatccttta catcagaaga ggagaggata cgaatgaagc aggagcagga 4381 gaggattcaa gccaaaactc gagaatttag ggaacgacaa gctcgagagc gtgactatgc 4441 tgaaattcaa gattttcatc ggacatttgg ctgtgatgat gagttaatgt atgggggagt 4501 ttcttcttat gaaggttcca tggctctcaa cgctagacct cagagcccac gagaagggca 4561 tatgatggat gctttgtatg cccaagtcaa gaagccgcgg aattccaaac cctcacctgt 4621 agacagtaac agatcaactc ctagcaatca tgatcggata cagcgtctga ggcaagaatt 4681 tcagcaagca aagcaagatg aagatgtaga agatcgtcgg cggacctata gttttgagca 4741 accctggccg aacgcacggc cggcgacgca gagcgggcga cactcggtgt ccgtggaggt 4801 gcagatgcag cggcagcggc aggaggagcg cgagagctcc cagcaggccc agcgccagta 4861 cagctctctg cctcggcaaa gcaggaaaaa tgccagctcg gtctcccagg actcttggga 4921 gcagaactac tcccctgggg aaggcttcca gagtgccaaa gagaacccca ggtactccag 4981 ctaccaaggc tccaggaacg gctacctggg aggacatggc ttcaacgcca gggtcatgct 5041 ggaaactcag gagctccttc gccaggaaca gaggcgaaag gagcagcaga tgaagaagca 5101 gcctccttcc gaggggccca gcaactatga ctcgtataag aaagtccagg accccagtta 5161 cgcccctccc aaggggccct tccggcaaga tgtgcccccc tccccttctc aggttgcgag 5221 gctgaacaga cttcagactc ctgagaaagg gaggcccttc tattcctgag cggccgcttg 5281 aattcatcga tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata 5341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5401 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc 5461 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa 5521 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 5581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 5641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 5701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 5761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 5821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc 5881 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 5941 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 6001 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt 6061 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 6121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 6181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 6241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 6301 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 6361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 6421 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 6481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 6541 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 6601 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 6661 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 6721 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 6781 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 6841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 6901 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 6961 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 7021 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 7081 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 7141 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 7201 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 7261 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 7321 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 7381 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 7441 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 7501 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 7561 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 7621 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 7681 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 7741 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 7801 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 7861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 7921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 7981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 8041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 8101 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 8161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 8221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 8281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 8341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 8401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 8461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 8521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 8581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 8641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 8701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 8761 gcccaatacg caaaccgcct c //