LOCUS       Exported               11697 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses a synthetic 
            cluster of miR-9/9* and miR-124 fused to Bcl-xL under a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pTight-9-124-BclxL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Victor MB, Richner M, Hermanstyne TO, Ransdell JL, Sobieski C, Deng 
            PY, Klyachko VA, Nerbonne JM, Yoo AS
  TITLE     Generation of Human Striatal Neurons by MicroRNA-Dependent Direct 
            Conversion of Fibroblasts.
  JOURNAL   Neuron. 2014 Oct 22;84(2):311-23. doi: 10.1016/j.neuron.2014.10.016.
            Epub 2014 Oct 22.
  PUBMED    25374357
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    122..140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    157..175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    193..211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        296..343
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        335..353
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      357..366
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             363..737
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    802..919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1217..1261)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1446..1679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2176..2301
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2348..2981
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3149..3283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3331..3435
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3436..4296
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3654..3673)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4467..5055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4956..4975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5209..5226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5343..5364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5379..5409
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5417..5433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5422..5444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5441..5457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5441..5457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5506..5627
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6835..7164)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6849..6984
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6903..6922)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7149..7169
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7178..7606)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7288..7309)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7500..7519
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7652..7876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7865..7882
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             7983..8216
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8423..9011
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8529..8540
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8938..8958
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9027..9629)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9110..9130)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9632..10204
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9791..9810)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     10020..10037
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(11602..219)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(11605..11629)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11676..11694
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aactcgacat acgttctcta tcactgatag ggataaactc gacatacgtt ctctatcact
       61 gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac tcgacatacg
      121 ttctctatca ctgataggga gtaaactcga catcgttctc tatcactgat agggagtaaa
      181 ctcgacatac gttctctatc actgataggg agtaaactcg agtgaagacg aaagggcctc
      241 atcgatggga tccgcgttaa ccaccggtct ctagaacctc ggacctgcag cacgtgttga
      301 caattaatca tcggcatagt atatcggcat agtataatac gactcactat aggagggcca
      361 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
      421 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
      481 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
      541 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
      601 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
      661 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
      721 ccgaggagca ggactgattc cggactagag cgggcccggt actaaccaaa ctggatctct
      781 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
      841 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
      901 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac
      961 gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     1021 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     1081 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     1141 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     1201 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     1261 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     1321 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     1381 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     1441 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     1501 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     1561 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     1621 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     1681 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     1741 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     1801 tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact
     1861 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     1921 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     1981 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     2041 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     2101 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     2161 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     2221 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     2281 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     2341 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     2401 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     2461 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     2521 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     2581 cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca
     2641 agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa
     2701 tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg
     2761 caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc
     2821 tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg
     2881 ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     2941 aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac
     3001 atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga
     3061 gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat
     3121 gattttggtt agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt
     3181 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3241 tattgcttta tttgtaacca ttataagctg caataaacaa gttgatctcc cgatccgtcg
     3301 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3361 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3421 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3481 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3541 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3601 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3661 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3721 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3781 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     3841 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     3901 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3961 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4021 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4081 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4141 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4201 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4261 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4321 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4381 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4441 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5101 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5161 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5221 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5281 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5341 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     5401 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     5461 gattacgcca agctctagct agaggtcgac ggtatacaga catgataaga tacattgatg
     5521 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5581 atgctattgc tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag
     5641 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     5701 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtag cacgtgctat
     5761 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     5821 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     5881 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     5941 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     6001 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     6061 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     6121 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     6181 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     6241 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     6301 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     6361 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     6421 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     6481 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattaa
     6541 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     6601 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     6661 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     6721 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     6781 tttcatcacg tgctgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca
     6841 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggcagaggc
     6901 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     6961 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     7021 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     7081 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     7141 ctttccacac cctaactgac acacattcca cagaattaat tcgcgttaaa tttttgttaa
     7201 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7261 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7321 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7381 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7441 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7501 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7561 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg atacccccta
     7621 gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
     7681 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     7741 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     7801 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     7861 caactagaag gcacagtcga ggctgatcag cgggtttaaa caatgtcaag gcctctcact
     7921 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     7981 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     8041 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     8101 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     8161 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc
     8221 cctcttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     8281 aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc
     8341 acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc
     8401 tcgactgcag aattaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga
     8461 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     8521 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     8581 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
     8641 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     8701 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     8761 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     8821 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     8881 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     8941 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     9001 agaggttgat tgttccagac gcgaactcag gcaccgggct tgcgggtcat gcaccaggtg
     9061 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     9121 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccagcgcg ctcggccgcc
     9181 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     9241 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     9301 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcga gccgatctcg
     9361 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     9421 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     9481 agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag
     9541 tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg
     9601 cgcaccgtgg gcttgtactc ggtggccatg gtattatcgt gtttttcaaa ggaaaaccac
     9661 gtccccgtgg ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca
     9721 tgtgcaccga ggccccagat cagatcccat acaatggggt accttctggg catccttcag
     9781 ccccttgttg aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac
     9841 aacgtggcac tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc
     9901 gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt
     9961 tgtttgtctt caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg
    10021 cattcctttg gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg
    10081 tttccgggcc ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa
    10141 cgcacaccgg ccttattcca agcggcttcg gccagtaacg ttaggggggg gggagggaga
    10201 ggggcggaat ttgcggccgt gtcgcacagg atgggttcat tctagaattg tttctcccca
    10261 cccttcctaa cttctctcgg atgtccttca agtgcagctg taggctccgc tcttggcatt
    10321 caccgcgtgc cttaattgta tgacattaaa tcaaggtccg ctgtgaacac ggagagcaga
    10381 gtctctgatc ttgatctaga agcaagtgac tcattgcaac tattactata attttctccc
    10441 tgcccttcac cctctcttgg agctctaggc ttggagtccc tactcctaga gttgtagctc
    10501 tgagcagtac tcccacctac tagaaggaga gccgaggtcg gatacctggt cccggcgtgg
    10561 ccgctccgtc gagctggaat ccctgggtgg cgtgggcttc tctgggctcc tctggctcct
    10621 gtggccaggg cggccggccg ggccatcgca cgcagaagtt gtgagagtca tttctacttt
    10681 cggttatcta gctttatgac ggctctgtgg cactcataca gctagataac caaagagaga
    10741 aacgggcctc ccattcgccg gcaccgctgc cgcgctgtgg gccccacgtg ccgccgccga
    10801 cccggcgcgg gggcctgagc ggactccgct cccgcagccc cgggcgcacg cggagagcca
    10861 gaggggaccg acaggccgcc gacgcgttca tttccgactg aagagtgagc ccagcagaac
    10921 cacgccggcc acagtcatgc ccgtcaggaa ccagcggttg aagcgttcct ggccctttcg
    10981 gctctcggct gctgcattgt tcccatagag ttccacaaaa gtatcccagc cgccgttctc
    11041 ctggatccaa ggctctaggt ggtcattcag gtaagtggcc atccaagctg cgatccgact
    11101 caccaatacc tgcatctcct tgtctacgct ttccacgcac agtgccccgc cgaaggagaa
    11161 aaaggccaca atgcgacccc agtttacccc atcccggaag agttcattca ctacctgttc
    11221 aaagctctga tatgctgtcc ctggggtgat gtggagctgg gatgtcaggt cactgaatgc
    11281 ccgccggtac cgcagttcaa actcgtcgcc tgcctccctc agcgcttgct ttactgctgc
    11341 catggggatc acctcccggg catccaaact gctgctgtgg ccagtggctc cattcaccgc
    11401 ggggctgtct gccaggtgcc aggatgggtt gccattgatg gcactggggg tctccatctc
    11461 cgattcagtc ccttctgggg cctcagtcct gttctcttcc acatcactaa actgactcca
    11521 gctgtatcct ttctgggaaa gcttgtagga gagaaagtca accaccagct cccggttgct
    11581 ctgagacatg gtggcagcgc tggcgatctg acggttcact aaacgagctc tgcttatata
    11641 ggcctcccac cgtacacgcc tacctcgaca tacgttctct atcactgata gggagta
//
