LOCUS Exported 6097 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expresses cytoplasmic ATP sensor in neurons with red reference protein. ACCESSION . VERSION . KEYWORDS Synapsin-cyto-mRuby3-iATPSnFR1.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6097) AUTHORS Lobas MA, Tao R, Nagai J, Kronschlager MT, Borden PM, Marvin JS, Looger LL, Khakh BS TITLE A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP. JOURNAL Nat Commun. 2019 Feb 12;10(1):711. doi: 10.1038/s41467-019-08441-5. PUBMED 30755613 REFERENCE 2 (bases 1 to 6097) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6097 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..448 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" primer_bind 1711..1732 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(1835..1856) /label=EGFP-N /note="EGFP, reverse primer" misc_feature 2324..2912 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2377..2397) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2795..2806) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2915..2931) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2916..2932) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 2961..3082 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2972..2991) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3026..3045 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 3244..3373 /label=AAV2 ITR primer_bind complement(3439..3456) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3610..4198) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3690..3709) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4369..5229) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4992..5011 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5230..5334) /gene="bla" /label=AmpR promoter rep_origin complement(5360..5815) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5497..5518) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5709..5728 /label=F1ori-R /note="F1 origin, reverse primer" repeat_region 5887..6016 /label=AAV2 ITR ORIGIN 1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga 61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga 121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg 181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc 241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg 301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg 361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg 421 aggagtcgtg tcgtgcctga gagcgcagtc gaattcaagc tgctagcaag gatccatggt 481 gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc 541 ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcaggccgt acgagggagt 601 gcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct 661 tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag tacccggcag acattcctga 721 tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga 781 tggtggagtc gtcaccgtca cgcaggacac cagccttgag gatggcgaac tcgtttacaa 841 cgtcaaagtc agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa 901 gggttgggag cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac 961 tgatattgca ctgaaagttg atggtggtgg ccatctgcat tgcaatttcg taacaactta 1021 caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg ttgatcaccg 1081 cctggaaagg atagaggaaa gtgacaatga aacgtacgta gtacaacgcg aagtcgcagt 1141 tgccaagtac tccaatcttg gtggtggatc catgaagact attcacgtga gtgtcgtaac 1201 tcccgacggg cctgtatatg aggatgacgt tgaaatggtg agcgtcaaag caaaaagtgg 1261 cgagctcggt attctcccag gccacattcc cttgaaagct cccctagaga tcagtgccgc 1321 acgcctgaag aaagggggca aaacacagta tatcgctgtg tcaggcggca acctcgaagt 1381 gcggcctgac aaggtgacca tctatgccca ggcagccgag agggctgagg atatcgatgt 1441 cctgcgcgcc aaggccgcca aggagagagc cgagcgccga ctccaatcac aggtcctgag 1501 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcga acttcaagat 1561 ccgccacaac gtggaggacg gcagcatgca gctcgccgac cactaccagc agaacacccc 1621 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgtgct 1681 gagcaaagac cctaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 1741 cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga gcatgagcaa 1801 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 1861 cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac 1921 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 1981 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 2041 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcagct tcaaggacga 2101 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 2161 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 2221 caactttggg ttgcacgaca tcgattttaa gcgcgccgag ctcgccctta agcgcgcaat 2281 gaataggctc tcagttgccg aaatgaagta gaagcttatc gataatcaac ctctggatta 2341 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 2401 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 2461 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 2521 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 2581 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 2641 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 2701 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg 2761 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 2821 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 2881 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg 2941 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat 3001 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 3061 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 3121 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga 3181 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact 3241 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 3301 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 3361 agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 4081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5341 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 5401 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 5461 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 5521 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 5581 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 5641 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 5701 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 5761 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 5821 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 5881 cgccagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 5941 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 6001 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 6061 tgctctagga agatctctgc agagggccct gcgtatg //