LOCUS Exported 10046 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA). ACCESSION . VERSION . KEYWORDS pUDP002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10046) AUTHORS Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG TITLE Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid. JOURNAL FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012. PUBMED 29438517 REFERENCE 2 (bases 1 to 10046) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10046 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 409..1437 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA KE" CDS 2016..2039 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2043..2063 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2064..6164 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6168..6188 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 7107..7773 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 7643..7666 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind complement(7835..7853) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" CDS complement(8287..9147) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8910..8929 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9148..9252) /gene="bla" /label=AmpR promoter rep_origin 9355..9943 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9844..9863 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac 61 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga 121 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata 181 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag 241 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga 301 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc 361 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gggtaaaaag 421 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc 481 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg 541 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt 601 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt 661 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg 721 cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct 781 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa 841 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa 901 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt 961 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat 1021 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg 1081 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag 1141 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg 1201 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc 1261 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact 1321 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa 1381 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataactc 1441 gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt 1501 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc 1561 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga 1621 atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga cgatgcccgt 1681 gtctaaatga ttcgaccagc ctaagaatgt tcaacaatct ataatcagtc catagtcaac 1741 aagagccctc aaagttgata tcgtttagtg atctccctcg agatgagcgg aggatggttg 1801 ctttttgggg agccctaagt ggtacgtgcg gcgagcggga tgtttcccca ttaggcaaag 1861 gacgaccggg taacccctcg agaaaaaaaa tttttcgccg ctaatctggt gttatataaa 1921 gctccccctg ctcctggaat ttttttcctt gtcaactcac accggaaatc aaggcatttc 1981 attctgagta gttcttaaaa acataatcaa caatggatta taaagatgac gatgacaaac 2041 ctccaaaaaa gaagagaaag gtcgataaga aatactcaat aggcttagat atcggcacaa 2101 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg 2161 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg 2221 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac 2281 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag 2341 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg 2401 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa 2461 ctatctatca tctgcgaaaa aaattggtag attctactta taaagcggat ttgcgcttaa 2521 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt 2581 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc 2641 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg 2701 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga 2761 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat 2821 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg 2881 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta 2941 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta 3001 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc 3061 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc 3121 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata 3181 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa 3241 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt acccatcaaa 3301 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa 3361 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc 3421 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta 3481 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac 3541 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc 3601 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa 3661 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca 3721 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat 3781 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct 3841 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag 3901 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg 3961 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc 4021 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc 4081 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta 4141 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt 4201 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta 4261 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc 4321 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc 4381 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc 4441 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct 4501 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa 4561 gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca 4621 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag 4681 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc 4741 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag 4801 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa 4861 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta 4921 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata 4981 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg 5041 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata 5101 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg 5161 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa 5221 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct 5281 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata 5341 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa 5401 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt 5461 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat 5521 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct 5581 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact 5641 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc 5701 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga 5761 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac 5821 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca 5881 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat 5941 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta 6001 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta 6061 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg 6121 gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccctcca aaaaagaaga 6181 gaaaggtctg agcggttttg tataactaaa taatattgga aactaaatac gaatacccaa 6241 attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga aacaggtaaa 6301 tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt attagtcgcc 6361 gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg tataagggaa 6421 actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg aaactacggc aaaggattgg 6481 tcagatcgct tcatacaggg aaagttcggc atcaacatct ttggataata tcagaatgag 6541 aaagaacaga tacgcagtac gttttttggt gagctctttg cacttcttta gttctttcca 6601 tcaatatcag ttttttaaac ttttaggact aaaagtgatg tttaacttca aaatgtttaa 6661 aattttgttc ttcccgacgt tcattaagaa tactaataca ctttaataat tattttaata 6721 ttttgttcta aataatgact tttaattaaa aaagataaaa tataaaaaca tcataataac 6781 tcaccagagg ttaagaacaa aaaaacaaat tagatatctg ctaatccaat atagttaaat 6841 caatctttcc ttggtataat gggtatatta catatatttc aaggaccgac actcctacca 6901 aatatctaaa atttaccata ttaacataac atgtatataa acgtcaaatc ataatcagca 6961 ctaacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc gaggacacgc 7021 tagggccaag gcaaaaagat tccttgatta cgtaagggag ttagaatcat tttgaataaa 7081 aaacacgctt tttcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta 7141 aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc 7201 tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta 7261 ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta 7321 agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca 7381 tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa 7441 aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc 7501 aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct 7561 ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac 7621 ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt 7681 aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac 7741 ttagtttcga ataaacacac ataaacaaac aaacgagacc tgcggaggtc tccacaggcc 7801 ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcccc 7861 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt 7921 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt 7981 ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt 8041 tgggacaagc ttacgtctca cggatcgtat atgccgtagc gacaatctaa gaactatgcg 8101 aggacacgct agcagctgta ttcacgtaga cggataggta tagccagaca tcagcagcat 8161 acttcgggaa ccgtaggcca ccgagcggcc gcgattatca aaaaggatct tcacctagat 8221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 8281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8401 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc 8461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 8521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 8941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 9181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 9241 aggggttccg cgcacatttc cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg 9301 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 9361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 9421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 9481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 9541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 9601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 9661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 9721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 9781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 9841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 9901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 9961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 10021 ccctgattct gtggataacc gtgcgg //