LOCUS Exported 717 bp ds-DNA linear SYN 16-SEP-2021 DEFINITION Anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3.1-R2ACR_853-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 717) AUTHORS Govorunova EG, Sineshchekov OA, Rodarte EM, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL TITLE The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. JOURNAL Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358. PUBMED 28256618 REFERENCE 2 (bases 1 to 717) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 16, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..717 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..717 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(43..64) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(304..323) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 651..672 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 181 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag 241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 601 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa //