LOCUS Exported 5434 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBAD33.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5434) AUTHORS Chung HS, Raetz CR TITLE Interchangeable domains in the Kdo transferases of Escherichia coli and Haemophilus influenzae. JOURNAL Biochemistry. 2010 May 18;49(19):4126-37. PUBMED 20394418 REFERENCE 2 (bases 1 to 5434) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5434 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 338..793 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(425..444) /label=F1ori-R /note="F1 origin, reverse primer" CDS complement(1348..2007) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1922..1941 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(2008..2110) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(2502..2519) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2636..3181) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(2716..2735) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3253..3272 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" CDS complement(3452..4330) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 4357..4641 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 4564..4583 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" RBS 4701..4723 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 4731..4763 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" primer_bind complement(4829..4846) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 4999..5085 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 5177..5204 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 5223..5314 /gene="bla" /label=AmpR promoter ORIGIN 1 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 61 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 301 cagaccaagt ttactcatat atactttaga ttgatttacg cgccctgtag cggcgcatta 361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 541 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt 601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aacttgaaca 661 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc 721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 781 acgtttacaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 1021 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtcag 1141 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa 1201 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt 1261 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcac caggcgttta 1321 agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg 1381 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg 1441 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac 1501 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca 1561 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt 1621 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg 1681 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg 1741 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg 1801 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt 1861 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg 1921 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt 1981 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa 2041 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac 2101 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg 2161 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca 2221 aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg 2281 ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc 2341 gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg cttactatgt 2401 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg 2461 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta 2521 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg 2581 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc 2641 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa 2701 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc 2761 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt 2821 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa 2881 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2941 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt 3001 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc 3061 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc 3121 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca 3181 agaagatcat cttattaatc agataaaata tttgctcatg agcccgaagt ggcgagcccg 3241 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 3301 gatgccggcc acgatgcgtc cggcgtagag gatctgctca tgtttgacag cttatcatcg 3361 atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg 3421 tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca 3481 ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata 3541 cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca 3601 tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta 3661 agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa 3721 catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact 3781 gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca 3841 tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt 3901 ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt 3961 catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc 4021 agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac 4081 gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa 4141 attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac 4201 ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg 4261 ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg 4321 cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca 4381 tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc 4441 cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca 4501 aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt 4561 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 4621 aactctctac tgtttctcca tacccgtttt tttgggctag cgaattcgag ctcggtaccc 4681 ggggatcctc tagaaataat tttgtttaac tttaagaagg agatatacat atggctagca 4741 tgactggtgg acagcaaatg ggtcgggatc cgaattcgag ctccgtcgac aagcttggct 4801 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg 4861 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc 4921 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag 4981 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 5041 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat 5101 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc 5161 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact 5221 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 5281 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 5341 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 5401 gaaagtaaaa gatgctgaag atcagttggg tgca //