LOCUS       Exported                 626 bp ds-DNA     linear   SYN 04-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJH548
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Sadofsky MJ, Hesse JE, McBlane JF, Gellert M
  TITLE     Expression and V(D)J recombination activity of mutated RAG-1 
            proteins.
  JOURNAL   Nucleic Acids Res. 1993 Dec 11. 21(24):5644-50.
  PUBMED    8284210
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     34..53
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     133..149
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     133..149
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
       61 agcttctaga gatccctcga cctcgaggga tcttccatac ctaccagttc tgcgcctgca
      121 ggtcgcggcc gctctagaac tagtggatcc aacatggctg cctccttgcc gtctaccctg
      181 agcttcagtt ctgcacccga tgaaattcaa cacccacaaa tcaaattttc cgagtggaaa
      241 tttaagctgt ttagggtgag atcctttgaa aaggcacccg aagaagcaca gaaggagaag
      301 gattcctcag aggggaaacc ttacctagaa cagtctccag tagttccaga gaagcctggt
      361 ggtcagaact caattctgac tcaacgagca ctgaaactcc atcctaaatt ttcaaagaaa
      421 ttccatgctg atgggaagtc aagcgacaaa gcagttcacc aagccaggct tagacacttc
      481 tgccgcatct gtgggaatcg tttctagagt gacgggcaca gccggagata cccagtccac
      541 gggcccgtgg acgctaaaac ccaaagtctt ttccgaaaga aggaaataag agtcacttcc
      601 tggccagacc tcattgccag gatttt
//
