LOCUS       Exported               15679 bp ds-DNA     circular SYN 14-FEB-2022
DEFINITION  Lentiviral expression of dCas9-VPR-mCherry fusion protein for 
            CRISPRa..
ACCESSION   .
VERSION     .
KEYWORDS    IGI-P0492 pHR-dCas9-NLS-VPR-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15679)
  TITLE     Corn Lab Cas9 plasmids
REFERENCE   2  (bases 1 to 15679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2228..2606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2607..2810
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2761..2781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2807..2831
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2919..2928
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2925..7028
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7041..7061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    7098..7122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7152..7301
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7326..7346
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7776..8132
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8151..8720
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             8721..8777
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8778..9485
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(8920..8938)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9188..9207
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    9543..10131
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9596..9616)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10014..10025)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10134..10150)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10135..10151)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10241..10474
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10499..10517)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10500..10517)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10807..10826)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11040..11059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11159..11181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11219..11237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11305..11409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11410..12270
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11628..11647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12441..13029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12930..12949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13183..13200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13270..13290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13275..13604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13455..13590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13517..13536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          14738..14803
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14933..14953
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    15378..15512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15415..15434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15469..15488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2281 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2341 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2401 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2461 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2521 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2581 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2641 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2701 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2761 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     2821 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     2881 accgatccag cctccggact ctagaggatc gaacccttgc caccatggac aagaagtact
     2941 ccattgggct cgctatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca
     3001 aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga
     3061 acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa
     3121 gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct
     3181 ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt
     3241 tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg
     3301 tggcgtacca tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta
     3361 ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg
     3421 gacacttcct catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta
     3481 tccaactggt tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag
     3541 ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca
     3601 tcgcacagct ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac
     3661 tcgggctgac ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac
     3721 tgagcaaaga cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt
     3781 acgcagacct ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc
     3841 tgcgagtgaa cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg
     3901 atgagcacca ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga
     3961 agtacaagga aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg
     4021 gagcaagcca ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca
     4081 ccgaggagct gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg
     4141 acaatggaag catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc
     4201 aagaggattt ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat
     4261 ttcggatacc ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga
     4321 ctcgcaaatc agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg
     4381 cctctgccca gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa
     4441 aggtgcttcc taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca
     4501 aggtcaaata cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga
     4561 aagctatcgt ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag
     4621 aagactattt caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc
     4681 gcttcaacgc atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact
     4741 tcctggacaa tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt
     4801 ttgaagatag ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca
     4861 aagtcatgaa acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac
     4921 tgatcaatgg gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg
     4981 atggatttgc caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg
     5041 aggacatcca gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta
     5101 atcttgcagg tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg
     5161 aactcgtcaa agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag
     5221 agaaccaaac tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag
     5281 agggtataaa agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc
     5341 ttcagaatga gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc
     5401 aggaactgga catcaatcgg ctctccgact acgacgtggc tgctatcgtg ccccagtctt
     5461 ttctcaaaga tgattctatt gataataaag tgttgacaag atccgataaa gctagaggga
     5521 agagtgataa cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc
     5581 tgctgaacgc caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag
     5641 gtggcctgtc tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc
     5701 agatcaccaa gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa
     5761 atgacaaact gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt
     5821 tcagaaagga ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg
     5881 atgcctacct gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat
     5941 ctgaatttgt ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg
     6001 agcaggaaat aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt
     6061 tcaagaccga gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa
     6121 acggagaaac aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg
     6181 tcctgtccat gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct
     6241 ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt
     6301 gggaccccaa gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg
     6361 tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca
     6421 tcacaatcat ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag
     6481 gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc
     6541 ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc
     6601 tggcactgcc ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca
     6661 aagggtctcc cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc
     6721 ttgatgagat catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta
     6781 acctcgataa ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg
     6841 cagaaaacat tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt
     6901 acttcgacac caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca
     6961 cactgattca tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg
     7021 gtggagacag cagggctgac cccaagaaga agaggaaggt gtcgccaggg atccgtcgac
     7081 ttgacgcgtt gatatcaaca agtttgtaca aaaaagcagg ctacaaagag gccagcggtt
     7141 ccggacgggc tgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg
     7201 atgattttga ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc
     7261 tcggcagtga cgcccttgat gatttcgacc tggacatgct gattaactct agaagttccg
     7321 gatctccgaa aaagaaacgc aaagttggta gccagtacct gcccgacacc gacgaccggc
     7381 accggatcga ggaaaagcgg aagcggacct acgagacatt caagagcatc atgaagaagt
     7441 cccccttcag cggccccacc gaccctagac ctccacctag aagaatcgcc gtgcccagca
     7501 gatccagcgc cagcgtgcca aaacctgccc cccagcctta ccccttcacc agcagcctga
     7561 gcaccatcaa ctacgacgag ttccctacca tggtgttccc cagcggccag atctctcagg
     7621 cctctgctct ggctccagcc cctcctcagg tgctgcctca ggctcctgct cctgcaccag
     7681 ctccagccat ggtgtctgca ctggctcagg caccagcacc cgtgcctgtg ctggctcctg
     7741 gacctccaca ggctgtggct ccaccagccc ctaaacctac acaggccggc gagggcacac
     7801 tgtctgaagc tctgctgcag ctgcagttcg acgacgagga tctgggagcc ctgctgggaa
     7861 acagcaccga tcctgccgtg ttcaccgacc tggccagcgt ggacaacagc gagttccagc
     7921 agctgctgaa ccagggcatc cctgtggccc ctcacaccac cgagcccatg ctgatggaat
     7981 accccgaggc catcacccgg ctcgtgacag gcgctcagag gcctcctgat ccagctcctg
     8041 cccctctggg agcaccaggc ctgcctaatg gactgctgtc tggcgacgag gacttcagct
     8101 ctatcgccga tatggatttc tcagccttgc tgggctctgg cagcggcagc cgggattcca
     8161 gggaagggat gtttttgccg aagcctgagg ccggctccgc tattagtgac gtgtttgagg
     8221 gccgcgaggt gtgccagcca aaacgaatcc ggccatttca tcctccagga agtccatggg
     8281 ccaaccgccc actccccgcc agcctcgcac caacaccaac cggtccagta catgagccag
     8341 tcgggtcact gaccccggca ccagtccctc agccactgga tccagcgccc gcagtgactc
     8401 ccgaggccag tcacctgttg gaggatcccg atgaagagac gagccaggct gtcaaagccc
     8461 ttcgggagat ggccgatact gtgattcccc agaaggaaga ggctgcaatc tgtggccaaa
     8521 tggacctttc ccatccgccc ccaaggggcc atctggatga gctgacaacc acacttgagt
     8581 ccatgaccga ggatctgaac ctggactcac ccctgacccc ggaattgaac gagattctgg
     8641 ataccttcct gaacgacgag tgcctcttgc atgccatgca tatcagcaca ggactgtcca
     8701 tcttcgacac atctctgttt gctactaact tcagcctgct gaagcaggct ggagacgtgg
     8761 aggagaaccc tggacctgtg agcaagggcg aggaggataa catggccatc atcaaggagt
     8821 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     8881 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     8941 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     9001 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     9061 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     9121 cctccctcca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     9181 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     9241 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     9301 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     9361 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     9421 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     9481 agtagggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     9541 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9601 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9661 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9721 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9781 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9841 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9901 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     9961 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10021 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10081 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
    10141 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
    10201 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    10261 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10321 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10381 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10441 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
    10501 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
    10561 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
    10621 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
    10681 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
    10741 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
    10801 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
    10861 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
    10921 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
    10981 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
    11041 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    11101 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    11161 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    11221 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    11281 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    11341 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    11401 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    11461 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    11521 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    11581 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    11641 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    11701 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    11761 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    11821 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    11881 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    11941 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    12001 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    12061 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    12121 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    12181 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    12241 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    12301 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    12361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    12421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    12481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    12541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    12601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    12661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    12721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    12781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    12841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    12901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    12961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    13021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    13081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    13141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    13201 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    13261 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    13321 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    13381 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    13441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    13501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    13561 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga
    13621 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
    13681 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
    13741 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
    13801 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
    13861 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
    13921 ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
    13981 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
    14041 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga
    14101 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
    14161 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg
    14221 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
    14281 tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg
    14341 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
    14401 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
    14461 tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
    14521 atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
    14581 gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac
    14641 tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata
    14701 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
    14761 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
    14821 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
    14881 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
    14941 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
    15001 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
    15061 tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
    15121 cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
    15181 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
    15241 ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
    15301 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    15361 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    15421 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    15481 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    15541 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    15601 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    15661 gtactacaaa cttagtagt
//
