LOCUS Exported 12066 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato. ACCESSION . VERSION . KEYWORDS pDONOR-SNCAe3-WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12066) AUTHORS Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC TITLE FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling. JOURNAL Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5. PUBMED 28988985 REFERENCE 2 (bases 1 to 12066) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12066 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 75..378 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..659 /label=chicken beta-actin promoter intron 660..1668 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" CDS 1727..2428 /codon_start=1 /product="monomeric blue fluorescent protein" /label=TagBFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" polyA_signal 2572..2627 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" repeat_region 4040..4074 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" enhancer 4420..4723 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4726..5004 /label=chicken beta-actin promoter intron 5005..6013 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" regulatory 6066..6075 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6072..6773 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" /label=dTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLYGMDELYK" primer_bind complement(6205..6223) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6473..6492 /label=mCherry-F /note="mCherry, forward primer" primer_bind 6667..6690 /label=DsRed1-C /note="DsRed1, forward primer" CDS 6774..6827 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6828..7424 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 7321..7341 /label=Puro-F /note="Puromycin resistance gene, forward primer" polyA_signal 7568..7623 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" repeat_region 8153..8215 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(9214..9234) /label=T3 /note="T3 promoter, forward primer" promoter complement(9214..9232) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9253..9269) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9253..9269) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9266..9288) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9277..9293 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9301..9331) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9346..9367 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin 9604..10192 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10093..10112 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10346..10363 /label=L4440 /note="L4440 vector, forward primer" CDS complement(10414..11274) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11037..11056 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11275..11379) /gene="bla" /label=AmpR promoter rep_origin complement(11405..11860) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11542..11563) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 11754..11773 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11987..12009 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 12001..12018 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 12002..12018 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 12028..12047 /label=T7 /note="T7 promoter, forward primer" promoter 12028..12046 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 361 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 421 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 481 gggggcgggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg 541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 661 gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc 721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg 781 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc 841 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg 901 tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg 961 ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg 1021 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 1081 ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc 1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc 1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc 1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt 1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga 1381 cttcctttgt cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta 1441 gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 1501 tgcgtcgccg cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac 1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc 1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac 1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc gccaccatga gcgagctgat 1741 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa 1801 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt 1861 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg 1921 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc 1981 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac 2041 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa 2101 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga 2161 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt 2221 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa 2281 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc 2341 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc 2401 tagcaaactg gggcacaagc ttaattaaca ctcctcaggt gcaggctgcc tatcagaagg 2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg 2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 2701 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa 2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 2941 ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggtttct gatcctgtgt 3001 agagacccta gtttgaatgt gcttatagcc tattattaca tgtgtaatgt tacataaatt 3061 acttaactcg gatttttaat ttcatcagct atttaaaatg ggcataatat aactatatta 3121 aatggctgtt atgaagatta aataagatga tatgtaaaat gtgttttttg tttgtttgtt 3181 tgtttgtctg tttgtttttt tgagacagag tcttgctctg ttacccaggc tggagtgcag 3241 tggcacaatc ttggctcact gcaagttcta cctcccgagt tcatgccatt ctcctgcctc 3301 agcccctccc aagtagctgg gactacaggc acccgccacc acgcctggct aattttttgt 3361 atttttggta gagatggggt ttcaccatat tagccaggat ggtctcgatc tcctgacctc 3421 gtgatctgcc cacctcggcc tcccaaattg ctgggattac aggcatgagc cactgcgccc 3481 agcctaaaat gtttttttac ataatgggtg ttcagcacat gttaaagcct tctctccatc 3541 cttcttccct tttgtttcat gggttgactg atctgtctct agtgctgtac ttttaaagct 3601 tctacagttc tgaattcaaa attatcttct cactgggccc cggtgttatc tcattctttt 3661 ttctcctctg taagttgaca tgtgatgtgg gaacaaaggg gataaagtca ttattttgtg 3721 ctaaaatcgt aattggagag gacctcctgt tagctgggct ttcttctatt tattgtggtg 3781 gttactggag ttccttcttc tagttttagg atatatatat atattttttt ctttccctga 3841 agatataata atatatatac ttctgaagat tgagattttt aaattagttg tattgaaaac 3901 tagctaatca gcaatttaag gctagcttga gacttatgtc ttgaatttgt ttttgtaggc 3961 tccaaaacca aggagggagt ggtgcatggt gtggcaacag gtaagctcca ttgtgcttat 4021 atcgaaagat gatatttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct 4081 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct 4141 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt 4201 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa 4261 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt 4321 atatatatat tttcttgtta tagatatcaa ctagaatgct agttattaat agtaatcaat 4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 4681 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca 4741 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 4801 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgccaggc 4861 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat 4921 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 4981 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc 5041 cgcgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 5101 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt 5161 tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg 5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg 5281 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc 5341 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa 5401 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg 5461 gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg 5521 cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg 5581 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg 5641 gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg 5701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct 5761 gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga 5821 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc 5881 agcctcgggg ctgccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt 5941 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 6001 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa 6061 aggccgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg 6121 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc 6181 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct 6241 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc 6301 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg 6361 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg 6421 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa 6481 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg 6541 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg 6601 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg 6661 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc 6721 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aaggagggca 6781 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctacc gagtacaagc 6841 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg 6901 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg 6961 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 7021 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 7081 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 7141 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 7201 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 7261 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 7321 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 7381 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacactcc tcaggtgcag 7441 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga 7501 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 7561 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 7621 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 7681 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 7741 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 7801 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 7861 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 7921 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggatccctc 7981 gacttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa 8041 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact 8101 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc 8161 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaaa 8221 gtatctagtg gttagtgtgg cccagtattc aagattccta tgaaattgta aaacaatcac 8281 tgagcattct aagaacatat cagtcttatt gaaactgaat tctttataaa gtatttttaa 8341 ataggtaaat attgattata aataaaaaat atacttgcca agaataatga gggctttgaa 8401 ttgataagct atgtttaatt tatagtaagt gggcatttaa atattctgac caaaaatgta 8461 ttgacaaact gctgacaaaa ataaaatgtg aatattgcca taattttaaa aaaagtaaaa 8521 tttctgttga ttacagtaaa atattttgac cttaaattat gttgattaca atattccttt 8581 gataattcag agtgcatttc aggaaacacc cttggacagt cagtaaattg tttattgtat 8641 ttatctttgt attgttatgg tatagctatt tgtacaaata ttattgtgca attattacat 8701 ttctgattat attattcatt tggcctaaat ttaccgagaa tttgaacaag tcaattaggt 8761 ttataatcaa gaaatatcaa aaatgatgaa aaggatgata atcatcatca gatgttgagg 8821 aagatgagga tgagagtgcc agaaatagag aaatcaaagg agaaccaaaa tttaacaaat 8881 taaaagccca cagacttgct gtaattaagt tttctgttgt aagtactcca cgtttcctgg 8941 cagatgtggt gaagcaaaag atataatcag aaatataatt tatatgatcg gaaagcatta 9001 aacacaatag tgcctataca aataaaatgt tcctatcact gacttctaaa atggaaatga 9061 ggacaatgat atgggaatct taatacagtg ttgtggatag gactaaaaac acaggagtca 9121 gatcttcttg gttcaacttc ctgcttactc cttaccagct gtgtgaacct agtatacgcg 9181 tatgcggccg cttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 9241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 9301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 9361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 9421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gcttaaaact 9481 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 9541 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 9601 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 9661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 9721 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 9781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 9841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 9901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 9961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 10021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 10081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 10141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 10201 caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc 10261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 10321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagaatcaat 10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 10921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 10981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 11041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 11101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 11161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 11221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 11281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 11341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 11401 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 11461 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 11521 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 11581 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 11641 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 11701 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 11761 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 11821 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg 11881 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 11941 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 12001 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggggcgc 12061 gccatt //