LOCUS Exported 12082 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP. ACCESSION . VERSION . KEYWORDS pDONOR-SNCAe3-A53T SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12082) AUTHORS Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC TITLE FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling. JOURNAL Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5. PUBMED 28988985 REFERENCE 2 (bases 1 to 12082) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12082 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 75..378 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..659 /label=chicken beta-actin promoter intron 660..1668 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" CDS 1727..2428 /codon_start=1 /product="monomeric blue fluorescent protein" /label=TagBFP /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" polyA_signal 2572..2627 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" repeat_region 4040..4074 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" enhancer 4420..4723 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4726..5004 /label=chicken beta-actin promoter intron 5005..6013 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" regulatory 6066..6075 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6072..6788 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6072..6788 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6117..6138) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6378..6397) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6725..6746 /label=EGFP-C /note="EGFP, forward primer" CDS 6789..6842 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6843..7439 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 7336..7356 /label=Puro-F /note="Puromycin resistance gene, forward primer" polyA_signal 7583..7638 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" repeat_region 8168..8230 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(9230..9250) /label=T3 /note="T3 promoter, forward primer" promoter complement(9230..9248) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9269..9285) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9269..9285) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9282..9304) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9293..9309 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9317..9347) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9362..9383 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin 9620..10208 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10109..10128 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10362..10379 /label=L4440 /note="L4440 vector, forward primer" CDS complement(10430..11290) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11053..11072 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11291..11395) /gene="bla" /label=AmpR promoter rep_origin complement(11421..11876) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11558..11579) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 11770..11789 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 12003..12025 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 12017..12034 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 12018..12034 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 12044..12063 /label=T7 /note="T7 promoter, forward primer" promoter 12044..12062 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 361 gtcatcgcta ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 421 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 481 gggggcgggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg 541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc 601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg 661 gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc 721 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg 781 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc 841 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg 901 tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg 961 ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg 1021 tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 1081 ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc 1141 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc 1201 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc 1261 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt 1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga 1381 cttcctttgt cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta 1441 gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 1501 tgcgtcgccg cgccgccgtc cccttctcca tctccagcct cggggctgcc gcagggggac 1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc 1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac 1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaaggcc gccaccatga gcgagctgat 1741 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa 1801 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt 1861 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg 1921 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc 1981 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac 2041 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa 2101 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga 2161 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt 2221 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa 2281 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc 2341 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc 2401 tagcaaactg gggcacaagc ttaattaaca ctcctcaggt gcaggctgcc tatcagaagg 2461 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg 2521 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 2581 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 2641 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 2701 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa 2761 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 2821 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 2881 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 2941 ttatggagat ccctcgacct gcagcccaag cttggatccc ctaggtttct gatcctgtgt 3001 agagacccta gtttgaatgt gcttatagcc tattattaca tgtgtaatgt tacataaatt 3061 acttaactcg gatttttaat ttcatcagct atttaaaatg ggcataatat aactatatta 3121 aatggctgtt atgaagatta aataagatga tatgtaaaat gtgttttttg tttgtttgtt 3181 tgtttgtctg tttgtttttt tgagacagag tcttgctctg ttacccaggc tggagtgcag 3241 tggcacaatc ttggctcact gcaagttcta cctcccgagt tcatgccatt ctcctgcctc 3301 agcccctccc aagtagctgg gactacaggc acccgccacc acgcctggct aattttttgt 3361 atttttggta gagatggggt ttcaccatat tagccaggat ggtctcgatc tcctgacctc 3421 gtgatctgcc cacctcggcc tcccaaattg ctgggattac aggcatgagc cactgcgccc 3481 agcctaaaat gtttttttac ataatgggtg ttcagcacat gttaaagcct tctctccatc 3541 cttcttccct tttgtttcat gggttgactg atctgtctct agtgctgtac ttttaaagct 3601 tctacagttc tgaattcaaa attatcttct cactgggccc cggtgttatc tcattctttt 3661 ttctcctctg taagttgaca tgtgatgtgg gaacaaaggg gataaagtca ttattttgtg 3721 ctaaaatcgt aattggagag gacctcctgt tagctgggct ttcttctatt tattgtggtg 3781 gttactggag ttccttcttc tagttttagg atatatatat atattttttt ctttccctga 3841 agatataata atatatatac ttctgaagat tgagattttt aaattagttg tattgaaaac 3901 tagctaatca gcaatttaag gctagcttga gacttatgtc ttgaatttgt ttttgtaggc 3961 tccaaaacca aggagggagt ggtgcatggt gtgacaacag gtaagctcca ttgtgcttat 4021 atcgaaagat gatatttaac cctagaaaga tagtctgcgt aaaattgacg catgcattct 4081 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct 4141 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt 4201 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa 4261 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt 4321 atatatatat tttcttgtta tagatatcaa ctagaatgct agttattaat agtaatcaat 4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 4681 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca 4741 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 4801 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgccaggc 4861 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat 4921 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 4981 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc 5041 cgcgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 5101 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt 5161 tcttttctgt ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg 5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg 5281 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc 5341 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa 5401 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg 5461 gctgtaaccc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg 5521 cggggctccg tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg 5581 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg 5641 gcggccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg 5701 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct 5761 gggaggcgcc gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga 5821 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc 5881 agcctcgggg ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt 5941 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 6001 ctttttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa 6061 aggccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 6121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 6181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 6241 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 6301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 6361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 6421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 6481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 6541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 6601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 6661 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 6721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 6781 tgtacaagga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 6841 ctaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac 6901 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc 6961 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 7021 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 7081 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 7141 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 7201 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 7261 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 7321 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 7381 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac 7441 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct 7501 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc 7561 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg 7621 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag 7681 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa 7741 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc 7801 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt 7861 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc 7921 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc 7981 aagcttggat ccctcgactt ttgttacttt atagaagaaa ttttgagttt ttgttttttt 8041 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt 8101 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga 8161 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat 8221 ctttctaggg ttaaagtatc tagtgattag tgtggcccag tattcaagat tcctatgaaa 8281 ttgtaaaaca atcactgagc attctaagaa catatcagtc ttattgaaac tgaattcttt 8341 ataaagtatt tttaaatagg taaatattga ttataaataa aaaatatact tgccaagaat 8401 aatgagggct ttgaattgat aagctatgtt taatttatag taagtgggca tttaaatatt 8461 ctgaccaaaa atgtattgac aaactgctga caaaaataaa atgtgaatat tgccataatt 8521 ttaaaaaaag taaaatttct gttgattaca gtaaaatatt ttgaccttaa attatgttga 8581 ttacaatatt cctttgataa ttcagagtgc atttcaggaa acacccttgg acagtcagta 8641 aattgtttat tgtatttatc tttgtattgt tatggtatag ctatttgtac aaatattatt 8701 gtgcaattat tacatttctg attatattat tcatttggcc taaatttacc gagaatttga 8761 acaagtcaat taggtttata atcaagaaat atcaaaaatg atgaaaagga tgataatcat 8821 catcagatgt tgaggaagat gaggatgaga gtgccagaaa tagagaaatc aaaggagaac 8881 caaaatttaa caaattaaaa gcccacagac ttgctgtaat taagttttct gttgtaagta 8941 ctccacgttt cctggcagat gtggtgaagc aaaagatata atcagaaata taatttatat 9001 gatcggaaag cattaaacac aatagtgcct atacaaataa aatgttccta tcactgactt 9061 ctaaaatgga aatgaggaca atgatatggg aatcttaata cagtgttgtg gataggacta 9121 aaaacacagg agtcagatct tcttggttca acttcctgct tactccttac cagctgtgtg 9181 taacctagta tacgcgtatg cggccgctta attaatccag cttttgttcc ctttagtgag 9241 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 9301 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 9361 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 9421 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 9481 ttgggcgctt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 9541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 9601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 9661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 9721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 9781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 9841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 9901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 9961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 10021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 10081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 10141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 10201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg 10261 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 10321 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 10381 aagcggaaga atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 10441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 10501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 10561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 10621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 10681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 10741 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 10801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 10861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 10921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 10981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 11041 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 11101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 11161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 11221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 11281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 11341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 11401 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 11461 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 11521 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 11581 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 11641 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 11701 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 11761 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 11821 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca 11881 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 11941 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 12001 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata 12061 gggcgaattg gggcgcgcca tt //