LOCUS Exported 5423 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Baculovirus expression for structure determination; may not be full ORF. ACCESSION . VERSION . KEYWORDS 5WCJ (METTL13) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5423) TITLE SGC Toronto Q4 2016, Q1 Q2 2017 REFERENCE 2 (bases 1 to 5423) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5423 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 2..456 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(89..108) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 298..319 /label=F1ori-F /note="F1 origin, forward primer" promoter 482..586 /gene="bla" /label=AmpR promoter CDS 587..1447 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(805..824) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1618..2206 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2107..2126 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2360..2377 /label=L4440 /note="L4440 vector, forward primer" mobile_element 2511..2735 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(3505..3533) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" promoter 3885..3976 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 3901..3918 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 4035..4052 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 4065..4085 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" polyA_signal 4913..5047 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4950..4969) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5004..5023 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 5076..5241 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg 301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg 2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct 2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga 2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag 2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt 2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga 2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac 2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg 2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg 2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca 2941 tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact 3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc 3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta 3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct 3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg 3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg 3301 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc 3361 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg 3421 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt 3481 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt 3541 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc 3601 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag 3661 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt 3721 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa 3781 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca ggactctagc 3841 tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg gagataatta 3901 aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat 3961 aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc gcggatctcg 4021 gtccgaaaac catgcatcat catcatcatc acagcagcgg cagagaaaac ttgtatttcc 4081 agggctgttg tgaacaccac aaagccatga tcgctggcct tgccctgctg agaaacccag 4141 agctactcct agagatccca ctggcattgt tggtggtagg cctgggcggg ggcagcctcc 4201 ccctctttgt ccacgatcat tttccaaagt cctgcattga tgctgtggag atcgatccct 4261 ccatgttgga agtggccacc cagtggtttg gcttctccca gagtgaccga atgaaggtcc 4321 acattgcaga tggcctggac tatatcgcca gcttggcagg aggaggagaa gcacggcctt 4381 gctacgatgt cataatgttt gatgttgaca gtaaggaccc aacactggga atgagttgtc 4441 cgcccccagc atttgtggag caatcttttc tacagaaggt taaaagcatc ttgactcctg 4501 aaggtgtttt tattctcaac cttgtgtgcc gagacttggg gctaaaagac tcagtgctgg 4561 ctgggctcaa ggcagtgttc cccctcctat atgtccggcg aattgagggt gaagtgaatg 4621 agatcctgtt ctgtcagctg caccctgagc aaaaacttgc cacaccagag ctcctagaaa 4681 cagcccaggc tttggagcgg accctgagga agcctgggag gggttgggat gacacgtatg 4741 tcttgtcaga tatgctcaag acggtgaaaa ttgtgtgatg acgaagcttg tcgagaagta 4801 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 4861 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 4921 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 4981 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 5041 ctggatctga tcactgatat cgcctaggag atccgaacca gataagtgaa atctagttcc 5101 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc 5161 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc 5221 caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca 5281 tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg tcacagaatg 5341 aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata tcaacgctta 5401 tttgcagcct gaatggcgaa tgg //