LOCUS       Exported                5638 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-pgk-Cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  TITLE     Aebischer lab's AAV-Cre
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        180..679
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(215..238)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     612..631
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1222..1242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2385..2861
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2584..2603)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2901..3041
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2901..3030
                     /label=AAV2 ITR
     rep_origin      3116..3571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3203..3222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3413..3434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3588..3607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3707..3729
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3767..3785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3853..3957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3958..4818
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4176..4195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4989..5577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5478..5497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgcgatcac gagactagcc tcgacgatgg tcgagtaccg
      181 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
      241 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
      301 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
      361 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
      421 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
      481 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
      541 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
      601 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
      661 ctcatctccg ggcctttcga cctctagcgg gatcggattc gaatcccggc cgggaacggt
      721 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      781 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      841 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
      901 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
      961 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1021 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1081 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1141 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1201 cgattgaatt ccaccatggt gcccaagaag aagaggaaag tctccaacct gctgactgtg
     1261 caccaaaacc tgcctgccct ccctgtggat gccacctctg atgaagtcag gaagaacctg
     1321 atggacatgt tcagggacag gcaggccttc tctgaacaca cctggaagat gctcctgtct
     1381 gtgtgcagat cctgggctgc ctggtgcaag ctgaacaaca ggaaatggtt ccctgctgaa
     1441 cctgaggatg tgagggacta cctcctgtac ctgcaagcca gaggcctggc tgtgaagacc
     1501 atccaacagc acctgggcca gctcaacatg ctgcacagga gatctggcct gcctcgccct
     1561 tctgactcca atgctgtgtc cctggtgatg aggagaatca gaaaggagaa tgtggatgct
     1621 ggggagagag ccaagcaggc cctggccttt gaacgcactg actttgacca agtcagatcc
     1681 ctgatggaga actctgacag atgccaggac atcaggaacc tggccttcct gggcattgcc
     1741 tacaacaccc tgctgcgcat tgccgaaatt gccagaatca gagtgaagga catctcccgc
     1801 accgatggtg ggagaatgct gatccacatt ggcaggacca agaccctggt gtccacagct
     1861 ggtgtggaga aggccctgtc cctgggggtt accaagctgg tggagagatg gatctctgtg
     1921 tctggtgtgg ctgatgaccc caacaactac ctgttctgcc gggtcagaaa gaatggtgtg
     1981 gctgcccctt ctgccacctc ccaactgtcc acccgggccc tggaagggat ctttgaggcc
     2041 acccaccgcc tgatctatgg tgccaaggat gactctgggc agagatacct ggcctggtct
     2101 ggccactctg ccagagtggg tgctgccagg gacatggcca gggctggtgt gtccatccct
     2161 gaaatcatgc aggctggtgg ctggaccaat gtgaacattg tgatgaacta catcagaaac
     2221 ctggactctg agactggggc catggtgagg ctgctcgagg atggggactg aaactgagtc
     2281 gagggggggc ccgggccgcg actctagatc ataatcagcc ataccacatt tctagagtcg
     2341 acctgcagaa gcttgcctcg agcagcgctg ctcgagagat ctacgggtgg catccctgtg
     2401 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     2461 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     2521 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     2581 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     2641 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     2701 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     2761 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     2821 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     2881 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2941 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3001 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3061 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     3121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3181 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3301 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     3361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3421 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     3481 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3541 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     3601 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3661 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3721 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4201 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4501 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
