LOCUS Exported 9557 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual. ACCESSION . VERSION . KEYWORDS pFastBac_Dual_SNF2L-opt_p48 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9557) TITLE pFastBac1_FLAG-BPTF JOURNAL Unpublished REFERENCE 2 (bases 1 to 9557) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9557 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 102..556 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(189..208) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 398..419 /label=F1ori-F /note="F1 origin, forward primer" promoter 582..686 /gene="bla" /label=AmpR promoter CDS 687..1547 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(905..924) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1718..2306 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2207..2226 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2460..2477 /label=L4440 /note="L4440 vector, forward primer" mobile_element 2611..2835 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" promoter complement(3605..3633) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" polyA_signal 4141..4189 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 4214..4233 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" promoter complement(7463..7572) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 7596..7687 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 7612..7629 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" polyA_signal 9147..9281 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9184..9203) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9238..9257 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 9310..9475 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga 421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg 2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc 3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3421 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg 3481 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt 3541 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca 3601 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca 3661 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct 3721 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt 3781 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg 3841 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt 3901 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct 3961 acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg agccctgggc 4021 cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg tattggtccc 4081 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat 4141 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 4201 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccggtacct 4261 tacgacttca ccttcttcac atccttcttg cccgacgatt cagtagccga ctcagccttt 4321 ctcttttggg acattggagt cttggtggca cgcttcttct tctcagcgcg ttcacgctct 4381 tcgatctcca tgttctcctt ttcgatcaga gagatcaaag tgttacatct cctctggaac 4441 tccatggcgg tgcgggactt gatgaaccag tcgaaacgga attgaggagc gttgcgaaca 4501 cactgacgca gctcttcgta gacgttctca cgatcgaatc ccatcttgtg caacatgcag 4561 atgaggaacc tgtcctcttc ctctgtgtag ttctttccct ttgaagtgcc gtattggatt 4621 ctcagctggt ggaagggggc cttgtacctg gcgatcttag cgtccaaggc cttcttgatg 4681 ctgatgcgac gctggattct ggcctcgccc ctttcgattt gagccatgat cttctcgatg 4741 tcctgcagct cgttgcaacg ttcccagaac acagccgagt actccataac ttcctcaggt 4801 gacttgccct ccacttctct ggcgatgtta tcgatatcgt cccttccgta cttctcgtta 4861 gccttgatga attggttgaa gtcacgctta gtccagttgg tgaagccctg agtcagcaac 4921 ttctcctttt cctcggtttc ctctggtgtg agaggttcag caccatcgat cttcttttgt 4981 tcctccctct gagccagggc agggtttggg atgtctgggt ttctcgggac cttgtaaccg 5041 atggtcttac ggtagtagag gatctccttt tcgagcagct cgaacagcct aggggggaag 5101 aattggaagt cctgcacgtt tggctgcttt ggtggtcttg gagccttagg gatcttgggc 5161 tcagagacgc gcagagcttc acggaagtaa gcgtccacgg cgtagttagc ctttctctcc 5221 ctcttagggg gctcgatcca ttcgaccatg ccgagctttt gcttttcgcg gtaatcctcg 5281 ccttcgaact tgtacaggga ctgctcgatg tccatgcgga agttacgcag gctagattct 5341 cccatcttct gcaagcgctc gttcatttca gcggtcttct tctcgccacg ttccaagatg 5401 gttgtgatat cctcgtcggt gagctctgat tccttgctgg cgaagacgtg tgtagcaccg 5461 tgacggatca tctgcagcat ttcctccttg gccaacttgt tggattgctg atcgatcagg 5521 cgtccttgct ggataacgat cgagtccaga cgcaacttga tctcagctct ttccacgatc 5581 ctttcctcaa ctgtgttgtc agtgatgagt ctgaacaccc taactggctt cttctggccg 5641 atgcggtggg cacgatccat agcttgcagg tccacctgag ggttccaatc tgagtcgtac 5701 aagatcacaa cgtcagcgct ggccaggttg atacccaaac ctccagcgcg ggtagacagc 5761 atgaagatga acttggacga gtttggggcg ttgaaagcct cgatggcttc ctcacgttcc 5821 tcgtgagggg tctgaccgtc gaggcgacag tactcgtatc cacgccacat gcagtaatct 5881 tccaagatgt ccaagagtct tgtcatttgg ctgaagatga gaaccctaga tccctgctcc 5941 ttgagcttgg ccagcaactt gtccaggacc accatcttgc cggagttcga gacgatgtgc 6001 tcatcagtgg tgtacggtgg accgggttca gctccgtcga acaggtacgg gtggttacag 6061 cacttgcgga gttgcatcag gatgttgagc agacgcatct tgtccatctt tcctgagctg 6121 ttcaagacat cgatgtcctt catgaggatc ttagtgtacc actccctctg catcttgctg 6181 aggcccaggt agatcttgat ctccttctta gggggcagag acttttccac gtctgtcttg 6241 attctcctca agaggaaagg cttgaggaca gcgtgcaggc gctccaccaa cttctggtca 6301 ccgaggcagt tcttagtatc gaaccaggag tcgaaatcgt cggccgagtt gaagacgtcg 6361 ggcagcaaga agttgagcag agcccacaac tcgtggaggt tgttctgcag tggtgttcca 6421 gtcaagagca gtctgttggt gctcttgaac tccctcacga tttcagacaa cttggacttc 6481 tcgttcttga ttctgtgggc ttcgtcgatg accaggtaac gccagtggaa cttcttgaaa 6541 acagacttct ccttgatgac catttcgtag gaagtgacac acacatccca ttcaccgggc 6601 atcatttcgt cgcggatgaa agcggcacga gcatccttgt ctccaacgaa gcagatgact 6661 ctcagcgatg gcacccaacg cttgaactcg ttcatccagt tgtggagggt tgacttcgga 6721 accaggacca tgtgcggtcc tgggatgttg cggtagtgct tcaggtagcc caagagggcg 6781 atggtttgga gtgtctttcc caggcccatc tcatcagcca agatgccgtt gacaccgttt 6841 tcgtacagag agatcaacca gttgaggcct ctgatctggt agtccctcag ggggccaccc 6901 ttgacgtatg acgggctcac ctcgaagcgg atgcacacgt tggatgtctt acgagactcg 6961 gacagcaatt cctcgtcttc ctcttgttca gtgcgacggt gacggtaatc gccagccgag 7021 atcagtgact gcttctcgtc cttcttgatg cgaggacgac ccaacttcat gttgagcggg 7081 ctagttggag acttttgggc tgagggctgg atgaagtgag cgaacagctc ggtctgcttg 7141 agcaggaatt cgaatctctt agccctatcg gccttcatct tttcctcgta ctctgggtcc 7201 atctcctttt cgctcttagg ggccttagcg gccaacttga gttggaaaga ggacacgttc 7261 ttctccttct tcttctcgcc cttttcagta gcggcggtag cttctgtagc agcagcagca 7321 gctccttcct cctgcgaggt tgagggaccc ggttgttcat cttcgataac gacgatagta 7381 gcggtggcat cagcagcagc gacggtagca gcgacggcag cagtgtcttg ttccatcccg 7441 ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta 7501 attaatatac aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt 7561 gaattaaagg tccgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa 7621 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc 7681 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatcgg gatggccgac 7741 aaggaagcag ccttcgacga cgcagtggaa gaacgagtga tcaacgagga atacaaaata 7801 tggaaaaaga acaccccttt tctttatgat ttggtgatga cccatgctct ggagtggccc 7861 agcctaactg cccagtggct tccagatgta accagaccag aagggaaaga tttcagcatt 7921 catcgacttg tcctggggac acacacatcg gatgaacaaa accatcttgt tatagccagt 7981 gtgcagctcc ctaatgatga tgctcagttt gatgcgtcac actacgacag tgagaaagga 8041 gaatttggag gttttggttc agttagtgga aaaattgaaa tagaaatcaa gatcaaccat 8101 gaaggagaag taaacagggc ccgttatatg ccccagaacc cttgtatcat cgcaacaaag 8161 actccttcca gtgatgttct tgtctttgac tatacaaaac atccttctaa accagatcct 8221 tctggagagt gcaacccaga cttgcgtctc cgtggacatc agaaggaagg ctatgggctt 8281 tcttggaacc caaatctcag tgggcactta cttagtgctt cagatgacca taccatctgc 8341 ctgtgggaca tcagtgccgt tccaaaggag ggaaaagtgg tagatgcgaa gaccatcttt 8401 acagggcata cggcagtagt agaagatgtt tcctggcatc tactccatga gtctctgttt 8461 gggtcagttg ctgatgatca gaaacttatg atttgggata ctcgttcaaa caatacttcc 8521 aaaccaagcc actcagttga tgctcacact gctgaagtga actgcctttc tttcaatcct 8581 tatagtgagt tcattcttgc cacaggatca gctgacaaga ctgttgcctt gtgggatctg 8641 agaaatctga aacttaagtt gcattccttt gagtcacata aggatgaaat attccaggtt 8701 cagtggtcac ctcacaatga gactatttta gcttccagtg gtactgatcg cagactgaat 8761 gtctgggatt taagtaaaat tggagaggaa caatccccag aagatgcaga agacgggcca 8821 ccagagttgt tgtttattca tggtggtcat actgccaaga tatctgattt ctcctggaat 8881 cccaatgaac cttgggtgat ttgttctgta tcagaagaca atatcatgca agtgtggcaa 8941 atggcagaga acatttataa tgatgaagac cctgaaggaa gcgtggatcc agaaggacaa 9001 gggtcctagg catgccccag cttgtcgaga agtactagag gatcataatc agccatacca 9061 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 9121 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 9181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 9241 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta 9301 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc 9361 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat 9421 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg 9481 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg 9541 tcatcttcgg ctacttt //