LOCUS       Exported                8898 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Hsp68-LacZ-Gateway
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Pennacchio LA, Ahituv N, Moses AM, Prabhakar S, Nobrega MA, Shoukry 
            M, Minovitsky S, Dubchak I, Holt A, Lewis KD, Plajzer-Frick I, 
            Akiyama J, De Val S, Afzal V, Black BL, Couronne O, Eisen MB, Visel 
            A, Rubin EM
  TITLE     In vivo enhancer analysis of human conserved non-coding sequences.
  JOURNAL   Nature. 2006 Nov 23;444(7118):499-502. Epub 2006 Nov 5.
  PUBMED    17086198
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     65..81
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     66..82
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    107..231
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        256..286
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             340..999
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(406..425)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1341..1646
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1525..1544
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1687..1811)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2728..5775
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    5923..6057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5960..5979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6014..6033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6054..6070)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6054..6070)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6105..6124)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6106..6124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6131..6148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6131..6147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(6288..6743)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6446)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6637..6656
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6770..6874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6875..7735
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7093..7112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7906..8494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8395..8414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8648..8665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8782..8803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8818..8848
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8856..8872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8861..8883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8880..8896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8880..8896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg taccgggccc
       61 cccctcgagg tcgacggtat cgataagctt gatatcgaat tccatcacaa gtttgtacaa
      121 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
      181 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
      241 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
      301 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      361 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      421 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      481 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      541 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
      601 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      661 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      721 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      781 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      841 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      901 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
      961 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     1021 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     1081 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     1141 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     1201 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     1261 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     1321 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     1381 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     1441 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     1501 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     1561 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     1621 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     1681 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     1741 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     1801 tacaaagtgg tgatgcccgg gggatccccg ggtaccgagc tccaggaaca tccaaactga
     1861 gcagccgggg tcccccccac cccccacccc gcccctcccg gcaactttga gcctgtgctg
     1921 ggacagagcc tctagttcct aaattagtcc atgaggtcag aggcagcact gccattgtaa
     1981 cgcgattgga gaggatcacg tcaccggaca cgcccccagg catctccctg ggtctcctaa
     2041 acttggcggg gagaagtttt agcccttaag ttttagcctt taacccccat attcagaact
     2101 gtgcgagttg gcgaaacccc acaaatcaca acaaactgta cacaacaccg agctagaggt
     2161 gatctttctt gtccattcca cacaggcctt agtaatgcgt cgccatagca acagtgtcac
     2221 tagtagcacc agcacttccc cacaccctcc ccctcaggaa tccgtactct ccagtgaacc
     2281 ccagaaacct ctggagagtt ctggacaagg gcggaaccca caactccgat tactcaaggg
     2341 aggcggggaa gctccaccag acgcgaaact gctggaagat tcctggcccc aaggcctcct
     2401 ccggctcgct gattggccca gcggagagtg ggcggggccg gtgaagactc cttaaaggcg
     2461 cagggcggcg agcaggtcac cagacgctga cagctactca gaaccaaatc tggttccatc
     2521 cagagacaag cgaagacaag agaagcagag cgagcggcgc gttcccgatc ctcggccagg
     2581 accagccttc cccagagcat ccctgccgcg gagcgcaacc ttcccaggag catccctgcc
     2641 gcggagcgca actttccccg gagcatccac gccgcggagc gcagccttcc agaagcagag
     2701 cgcggcgcca tggctcgcga tgatcccgtc gttttacaac gtcgtgactg ggaaaaccct
     2761 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2821 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2881 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
     2941 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
     3001 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
     3061 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
     3121 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc
     3181 ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
     3241 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
     3301 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
     3361 acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
     3421 ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
     3481 ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
     3541 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
     3601 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
     3661 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
     3721 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
     3781 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
     3841 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
     3901 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
     3961 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
     4021 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
     4081 aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
     4141 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt
     4201 tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc
     4261 atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc
     4321 cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
     4381 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
     4441 gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
     4501 cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa
     4561 gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
     4621 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
     4681 ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
     4741 attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc
     4801 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag
     4861 tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat
     4921 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
     4981 cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg
     5041 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
     5101 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
     5161 gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct
     5221 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat
     5281 ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg
     5341 gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga
     5401 ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat
     5461 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
     5521 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
     5581 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
     5641 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
     5701 agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc
     5761 tggtgtcaaa aataataata accgggcagg ggggatctaa gctctagata agtaatgatc
     5821 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5881 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccac
     6061 tagttctaga gcggccgcca ccgcggtgcg tcgacgccaa ttcgccctat agtgagtcgt
     6121 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     6301 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6361 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6421 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6481 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6541 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6601 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6661 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6721 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6841 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6901 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6961 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7021 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7081 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7141 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7201 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7261 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7321 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7381 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7441 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7501 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7561 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7621 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7681 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7741 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7801 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7861 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7921 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7981 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8041 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8101 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8161 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8221 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8281 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8341 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8401 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8461 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8521 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8581 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8641 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8701 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8761 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8821 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8881 aggaaacagc tatgacca
//
