LOCUS Exported 10219 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION transient expression of dCas9-dHDAC1 fusion protein. ACCESSION . VERSION . KEYWORDS p-dCas9-HDAC1-H141A-Hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10219) AUTHORS Stephan Beck TITLE Beck lab Cas9 plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 10219) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10219 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 86..207 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(97..116) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 151..170 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(238..1263) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(1281..1780) /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(1329..1348) /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 1722..1745 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" enhancer 1785..2164 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2165..2368 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2319..2339 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2410..2429 /label=T7 /note="T7 promoter, forward primer" promoter 2410..2428 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 2436..2445 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2442..6545 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 6552..6572 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6579..6644 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" primer_bind complement(7827..7844) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7833..8057 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(8128..8144) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8128..8144) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8141..8163) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8152..8168 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8176..8206) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8221..8242 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8359..8376) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8530..9118) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8610..8629) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9289..10149) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9912..9931 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10150..35) /gene="bla" /label=AmpR promoter ORIGIN 1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 61 gacgtcgacg ggtatacaga catgataaga tacattgatg agtttggaca aaccacaact 121 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 181 accattataa gctgcaataa acaagttggg gtgggcgaag aactctcggc atctactcta 241 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac 301 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc 361 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt 421 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag 481 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat 541 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa 601 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt 661 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat 721 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca 781 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc 841 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat 901 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc 961 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc 1021 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata 1081 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc 1141 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac 1201 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt 1261 catggtggcg ggatgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca 1321 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc 1381 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc 1441 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat 1501 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac 1561 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa 1621 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa 1681 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg 1741 catgctccag actgccttgg gaaaagcgcc tcccctaccc ggttgacatt gattattgac 1801 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 1861 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 1921 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 1981 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 2041 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 2101 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 2161 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 2221 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 2281 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 2341 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc 2401 cgcggccgct aatacgactc actataggga gagccgccac catggataaa aagtattcta 2461 ttggtttagc catcggcact aattccgttg gatgggctgt cataaccgat gaatacaaag 2521 taccttcaaa gaaatttaag gtgttgggga acacagaccg tcattcgatt aaaaagaatc 2581 ttatcggtgc cctcctattc gatagtggcg aaacggcaga ggcgactcgc ctgaaacgaa 2641 ccgctcggag aaggtataca cgtcgcaaga accgaatatg ttacttacaa gaaattttta 2701 gcaatgagat ggccaaagtt gacgattctt tctttcaccg tttggaagag tccttccttg 2761 tcgaagagga caagaaacat gaacggcacc ccatctttgg aaacatagta gatgaggtgg 2821 catatcatga aaagtaccca acgatttatc acctcagaaa aaagctagtt gactcaactg 2881 ataaagcgga cctgaggtta atctacttgg ctcttgccca tatgataaag ttccgtgggc 2941 actttctcat tgagggtgat ctaaatccgg acaactcgga tgtcgacaaa ctgttcatcc 3001 agttagtaca aacctataat cagttgtttg aagagaaccc tataaatgca agtggcgtgg 3061 atgcgaaggc tattcttagc gcccgcctct ctaaatcccg acggctagaa aacctgatcg 3121 cacaattacc cggagagaag aaaaatgggt tgttcggtaa ccttatagcg ctctcactag 3181 gcctgacacc aaattttaag tcgaacttcg acttagctga agatgccaaa ttgcagctta 3241 gtaaggacac gtacgatgac gatctcgaca atctactggc acaaattgga gatcagtatg 3301 cggacttatt tttggctgcc aaaaacctta gcgatgcaat cctcctatct gacatactga 3361 gagttaatac tgagattacc aaggcgccgt tatccgcttc aatgatcaaa aggtacgatg 3421 aacatcacca agacttgaca cttctcaagg ccctagtccg tcagcaactg cctgagaaat 3481 ataaggaaat attctttgat cagtcgaaaa acgggtacgc aggttatatt gacggcggag 3541 cgagtcaaga ggaattctac aagtttatca aacccatatt agagaagatg gatgggacgg 3601 aagagttgct tgtaaaactc aatcgcgaag atctactgcg aaagcagcgg actttcgaca 3661 acggtagcat tccacatcaa atccacttag gcgaattgca tgctatactt agaaggcagg 3721 aggattttta tccgttcctc aaagacaatc gtgaaaagat tgagaaaatc ctaacctttc 3781 gcatacctta ctatgtggga cccctggccc gagggaactc tcggttcgca tggatgacaa 3841 gaaagtccga agaaacgatt actccatgga attttgagga agttgtcgat aaaggtgcgt 3901 cagctcaatc gttcatcgag aggatgacca actttgacaa gaatttaccg aacgaaaaag 3961 tattgcctaa gcacagttta ctttacgagt atttcacagt gtacaatgaa ctcacgaaag 4021 ttaagtatgt cactgagggc atgcgtaaac ccgcctttct aagcggagaa cagaagaaag 4081 caatagtaga tctgttattc aagaccaacc gcaaagtgac agttaagcaa ttgaaagagg 4141 actactttaa gaaaattgaa tgcttcgatt ctgtcgagat ctccggggta gaagatcgat 4201 ttaatgcgtc acttggtacg tatcatgacc tcctaaagat aattaaagat aaggacttcc 4261 tggataacga agagaatgaa gatatcttag aagatatagt gttgactctt accctctttg 4321 aagatcggga aatgattgag gaaagactaa aaacatacgc tcacctgttc gacgataagg 4381 ttatgaaaca gttaaagagg cgtcgctata cgggctgggg acgattgtcg cggaaactta 4441 tcaacgggat aagagacaag caaagtggta aaactattct cgattttcta aagagcgacg 4501 gcttcgccaa taggaacttt atgcagctga tccatgatga ctctttaacc ttcaaagagg 4561 atatacaaaa ggcacaggtt tccggacaag gggactcatt gcacgaacat attgcgaatc 4621 ttgctggttc gccagccatc aaaaagggca tactccagac agtcaaagta gtggatgagc 4681 tagttaaggt catgggacgt cacaaaccgg aaaacattgt aatcgagatg gcacgcgaaa 4741 atcaaacgac tcagaagggg caaaaaaaca gtcgagagcg gatgaagaga atagaagagg 4801 gtattaaaga actgggcagc cagatcttaa aggagcatcc tgtggaaaat acccaattgc 4861 agaacgagaa actttacctc tattacctac aaaatggaag ggacatgtat gttgatcagg 4921 aactggacat aaaccgttta tctgattacg acgtcgatgc cattgtaccc caatcctttt 4981 tgaaggacga ttcaatcgac aataaagtgc ttacacgctc ggataagaac cgagggaaaa 5041 gtgacaatgt tccaagcgag gaagtcgtaa agaaaatgaa gaactattgg cggcagctcc 5101 taaatgcgaa actgataacg caaagaaagt tcgataactt aactaaagct gagaggggtg 5161 gcttgtctga acttgacaag gccggattta ttaaacgtca gctcgtggaa acccgccaaa 5221 tcacaaagca tgttgcacag atactagatt cccgaatgaa tacgaaatac gacgagaacg 5281 ataagctgat tcgggaagtc aaagtaatca ctttaaagtc aaaattggtg tcggacttca 5341 gaaaggattt tcaattctat aaagttaggg agataaataa ctaccaccat gcgcacgacg 5401 cttatcttaa tgccgtcgta gggaccgcac tcattaagaa atacccgaag ctagaaagtg 5461 agtttgtgta tggtgattac aaagtttatg acgtccgtaa gatgatcgcg aaaagcgaac 5521 aggagatagg caaggctaca gccaaatact tcttttattc taacattatg aatttcttta 5581 agacggaaat cactctggca aacggagaga tacgcaaacg acctttaatt gaaaccaatg 5641 gggagacagg tgaaatcgta tgggataagg gccgggactt cgcgacggtg agaaaagttt 5701 tgtccatgcc ccaagtcaac atagtaaaga aaactgaggt gcagaccgga gggttttcaa 5761 aggaatcgat tcttccaaaa aggaatagtg ataagctcat cgctcgtaaa aaggactggg 5821 acccgaaaaa gtacggtggc ttcgatagcc ctacagttgc ctattctgtc ctagtagtgg 5881 caaaagttga gaagggaaaa tccaagaaac tgaagtcagt caaagaatta ttggggataa 5941 cgattatgga gcgctcgtct tttgaaaaga accccatcga cttccttgag gcgaaaggtt 6001 acaaggaagt aaaaaaggat ctcataatta aactaccaaa gtatagtctg tttgagttag 6061 aaaatggccg aaaacggatg ttggctagcg ccggagagct tcaaaagggg aacgaactcg 6121 cactaccgtc taaatacgtg aatttcctgt atttagcgtc ccattacgag aagttgaaag 6181 gttcacctga agataacgaa cagaagcaac tttttgttga gcagcacaaa cattatctcg 6241 acgaaatcat agagcaaatt tcggaattca gtaagagagt catcctagct gatgccaatc 6301 tggacaaagt attaagcgca tacaacaagc acagggataa acccatacgt gagcaggcgg 6361 aaaatattat ccatttgttt actcttacca acctcggcgc tccagccgca ttcaagtatt 6421 ttgacacaac gatagatcgc aaacgataca cttctaccaa ggaggtgcta gacgcgacac 6481 tgattcacca atccatcacg ggattatatg aaactcggat agatttgtca cagcttgggg 6541 gtgacggatc ccccaagaag aagaggaaag tctcgagcga ctacaaagac catgacggtg 6601 attataaaga tcatgacatc gattacaagg atgacgatga caaggctgca ggaggcggag 6661 gtagcacgca gggcacccgg aggaaagtct gttactacta cgacggggat gttggaaatt 6721 actattatgg acaaggccac ccaatgaagc ctcaccgaat ccgcatgact cataatttgc 6781 tgctcaacta tggtctctac cgaaaaatgg aaatctatcg ccctcacaaa gccaatgctg 6841 aggagatgac caagtaccac agcgatgact acattaaatt cttgcgctcc atccgtccag 6901 ataacatgtc ggagtacagc aagcagatgc agagattcaa cgttggtgag gactgtccag 6961 tattcgatgg cctgtttgag ttctgtcagt tgtctactgg tggttctgtg gcaagtgctg 7021 tgaaacttaa taagcagcag acggacatcg ctgtgaattg ggctgggggc ctgcatgctg 7081 caaagaagtc cgaggcatct ggcttctgtt acgtcaatga tatcgtcttg gccatcctgg 7141 aactgctaaa gtatcaccag agggtgctgt acattgacat tgatattcac catggtgacg 7201 gcgtggaaga ggccttctac accacggacc gggtcatgac tgtgtccttt cataagtatg 7261 gagagtactt cccaggaact ggggacctac gggatatcgg ggctggcaaa ggcaagtatt 7321 atgctgttaa ctacccgctc cgagacggga ttgatgacga gtcctatgag gccattttca 7381 agccggtcat gtccaaagta atggagatgt tccagcctag tgcggtggtc ttacagtgtg 7441 gctcagactc cctatctggg gatcggttag gttgcttcaa tctaactatc aaaggacacg 7501 ccaagtgtgt ggaatttgtc aagagcttta acctgcctat gctgatgctg ggaggcggtg 7561 gttacaccat tcgtaacgtt gcccggtgct ggacatatga gacagctgtg gccctggata 7621 cggagatccc taatgagctt ccatacaatg actactttga atactttgga ccagatttca 7681 agctccacat cagtccttcc aatatgacta accagaacac gaatgagtac ctggagaaga 7741 tcaaacagcg actgtttgag aaccttagaa tgctgccgca cgcacctggg gtccaaatgc 7801 aggcgatttg aaaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 7861 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 7921 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 7981 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 8041 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac 8101 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 8161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta 8221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8701 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 8761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 9001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 9241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 9541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 10021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 10081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 10141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 10201 gagcggatac atatttgaa //