LOCUS Exported 1350 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch. ACCESSION . VERSION . KEYWORDS pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1350) AUTHORS Dana H, Sun Y, Mohar B, Hulse BK, Kerlin AM, Hasseman JP, Tsegaye G, Tsang A, Wong A, Patel R, Macklin JJ, Chen Y, Konnerth A, Jayaraman V, Looger LL, Schreiter ER, Svoboda K, Kim DS TITLE High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. JOURNAL Nat Methods. 2019 Jul;16(7):649-657. doi: 10.1038/s41592-019-0435-6. Epub 2019 Jun 17. PUBMED 31209382 REFERENCE 2 (bases 1 to 1350) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1350 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..1350 /codon_start=1 /product="improved fluorescent protein-based calcium sensor (Chen et al., 2013)" /label=GCaMP6f /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE LKGIDFKEDGNILGHKLEYNPPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMYARKMKYRDTEEEIREAFGVFDKDGN GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" regulatory 97..106 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind 387..408 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(514..535) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(775..794) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg 61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt 121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag 181 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc 241 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc 301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg 361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 421 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag 481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 541 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc 601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 721 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac 781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 841 gagcttaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 901 aacccacctg accaactgac tgaagagcag atcgcagaat ttaaagagct tttctcccta 961 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct 1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac 1081 ggtgacggca caatcgactt ccctgagttc ctgacaatgt acgcaagaaa aatgaaatac 1141 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc 1201 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat 1261 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac 1321 gaagagtttg tacaaatgat gacagcgaag //