LOCUS Exported 9281 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of EphA2 in mammalian cells. ACCESSION . VERSION . KEYWORDS Y593E EphA2 pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9281) AUTHORS Fang WB, Brantley-Sieders DM, Hwang Y, Ham AJ, Chen J TITLE Identification and functional analysis of phosphorylated tyrosine residues within EphA2 receptor tyrosine kinase. JOURNAL J Biol Chem. 2008 Jun 6;283(23):16017-26. doi: 10.1074/jbc.M709934200. Epub 2008 Apr 3. PUBMED 18387945 REFERENCE 2 (bases 1 to 9281) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9281 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(45..63) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(46..63) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(83..100) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 89..313 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 359..787 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(446..465) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 656..677 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(796..816) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 840..1050 /label=SV40 promoter /note="SV40 early promoter" promoter 862..1058 /label=SV40 promoter /note="SV40 early promoter" rep_origin 909..1044 /label=SV40 ori /note="SV40 origin of replication" primer_bind 971..990 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1125..1919 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(1179..1198) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1789..1808 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 2093..2214 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2130..2149) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2184..2203 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(2263..2279) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2263..2279) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2276..2298) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2287..2303 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2311..2341) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2356..2377 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2494..2511) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2665..3253) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2745..2764) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3424..4284) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4047..4066 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4285..4389) /gene="bla" /label=AmpR promoter primer_bind complement(4464..4483) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 4655..5034 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5035..5238 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 5189..5209 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 5283..5302 /label=T7 /note="T7 promoter, forward primer" promoter 5283..5301 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta 61 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 301 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 361 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 421 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 481 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 541 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 601 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 661 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 721 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 781 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 841 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 961 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 1021 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 1081 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 1141 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 1201 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 1261 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 1321 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 1381 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 1441 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 1501 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 1561 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 1621 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 1681 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 1741 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 1801 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 1861 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 1921 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 1981 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 2041 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 2101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 2161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 2221 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 2281 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 2341 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 2401 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 2461 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 2521 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 2581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 2641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 2701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 2761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 2821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 2881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 2941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 3121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 3181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 3241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 3301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 3361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 3421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 3601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 3661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 3721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 3781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 3841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 3901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 3961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 4021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 4081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 4141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 4201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 4321 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4381 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc 4441 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat 4501 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca 4561 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg 4621 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa 4681 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 4741 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 4801 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 4861 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 4921 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 4981 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 5041 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 5101 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 5161 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 5221 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa 5281 attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat ccactagtaa 5341 cggccgccag tgtgctggaa ttccgtagaa gttgtctctg tcggcgggcg ggcgggcagg 5401 attggggcac cgagaccggc gtgcggacag cagggatcgc ggggagcgag gggtgcggca 5461 tggagctccg ggcagtcggt ttctgcctgg cgctgctgtg gggttgcgcg ctggcggccg 5521 cggcggcaca gggaaaggaa gttgttttgt tggacttcgc agcaatgaag ggagagctcg 5581 gctggctcac gcacccctat ggcaaagggt gggacctgat gcagaacatc atggacgaca 5641 tgcctatcta catgtactcg gtgtgcaacg tggtatccgg cgaccaggac aactggctcc 5701 gcaccaactg ggtgtaccgg gaggaggccg agcgcatctt tattgagctc aagttcacgg 5761 tgcgagactg taacagcttc ccgggtggcg ccagctcctg caaagagacc ttcaacctct 5821 actatgcaga gtcagatgtg gactatggca ccaacttcca gaagcgccag ttcaccaaga 5881 ttgacaccat cgcccctgac gagatcacgg tcagcagtga cttcgaggct cgcaacgtca 5941 agctgaacgt agaggagcgc atggtggggc cccttacccg gaagggcttc tacctggcct 6001 tccaggacat cggcgcctgc gtggcgctgc tctccgttcg cgtctactac aagaagtgtc 6061 ccgagatgct gcagagcttg gctcgcttcc ccgagaccat tgctgtcgct gtttccgata 6121 cacaacccct ggccacggtg gccggtacct gcgtggacca tgccgtggtg ccttatgggg 6181 gcgaggggcc tctcatgcac tgcacggtgg atggcgagtg gctggtgccc atcgggcagt 6241 gcctgtgcca ggaaggctac gagaaggtcg aggatgcctg ccgagcctgt tctccaggat 6301 tcttcaagtc tgaggcatct gagagccctt gcctggagtg tccagagcat accctgccat 6361 ccacagaggg tgccacctcc tgccagtgtg aagaaggcta tttcagggca cctgaggacc 6421 cactgtccat gtcttgcaca cgtccaccct ctgcccctaa ctacctcacg gccattggca 6481 tgggtgccaa agtagaactg cgttggacag ctcccaagga cactggtggc cgccaggaca 6541 ttgtctacag tgtcacttgt gaacagtgct ggccagagtc tggcgagtgt gggccctgtg 6601 aggccagcgt gcgctattca gaacctcctc acgccctgac ccgcacgagt gtgacagtca 6661 gtgacctgga gccccacatg aactatacct tcgctgtcga agcacgcaat ggcgtctcag 6721 gcctggtgac tagccgaagc ttccggactg ccagcgtcag tattaaccaa acagagcccc 6781 ccaaagtgag gctggaggac cgaagcacca cctccctgag tgtcacctgg agcatcccgg 6841 tgtcacagca gagccgtgtg tggaagtacg aagtcaccta ccgcaagaag ggggatgcca 6901 acagctataa tgtgcgccgc acggaaggct tctccgtgac cctggatgac cttgctccgg 6961 ataccacgta cctggtgcag gtgcaggcgc tgacgcagga gggccaagga gccggcagca 7021 aagtgcacga gttccagacg ctgtccacgg aaggatctgc caacatggcg gtgatcggcg 7081 gtgtggctgt aggtgttgtt ttgcttctgg tactggcagg agttggcctc ttcatccatc 7141 gaaggaggag gaacctgcgg gctcgccagt cctctgagga tgtccgtttt tccaagtcag 7201 aacaactaaa gcccctgaag acctatgtgg atcctcacac tgaggaagac cccaaccagg 7261 ctgtactcaa gtttaccacc gagatccacc catcctgtgt ggcaaggcag aaggtcattg 7321 gagcaggaga gtttggagag gtctataaag ggacgctgaa ggcatcctcg gggaagaagg 7381 agataccggt ggccatcaag acactgaaag cgggctacac tgagaagcag cgggtggact 7441 tcctgagcga ggccagcatc atgggccagt ttagccacca caatatcatc cgcctggagg 7501 gcgtggtctc taaatacaaa cccatgatga ttatcacaga gtacatggag aatggagcgc 7561 tagacaagtt ccttagggag aaggatggtg agttcagcgt acttcagttg gtgggcatgc 7621 tgaggggtat cgcatccggc atgaagtacc tggccaacat gaactacgtg caccgggacc 7681 tggctgcccg caacatcctc gtcaacagca acctggtgtg caaggtgtcc gattttggcc 7741 tgtcgcgtgt gctggaggat gaccccgagg ccacctacac cacaagtggc ggcaagatcc 7801 ctattcgatg gacagcccca gaggccattt cctaccgcaa gttcacctca gccagcgatg 7861 tgtggagcta cggcattgtc atgtgggaag tgatgactta tggcgaacgg ccctactggg 7921 aactgtcaaa ccacgaggtc atgaaagcca tcaacgacgg cttccggctc cccacgccca 7981 tggactgccc ttcagccatt taccagctca tgatgcagtg ctggcagcaa gagcgctccc 8041 gccggcccaa gtttgccgac atcgttagca tcctggacaa gctcatccga gcccccgact 8101 ccctcaagac gctggctgac ttcgatcccc gagtgtccat ccggctgccc agcaccagcg 8161 gctcggaggg agtccccttc cgtacggtgt ccgagtggct ggagagcatc aagatgcaac 8221 agtacacgga acacttcatg gtggctggct acacggccat cgagaaggtg gtacagatgt 8281 ccaacgaaga catcaaaagg atcggagtgc gtcttcctgg ccaccagaag cgcattgcct 8341 acagcctgct gggactcaag gaccaggtca acacagtggg gattcctatc tgagtccatt 8401 ggggctgtgc ccaacaatac ttgaagagcc acagtggtct ccctgccgac ctggtgctgg 8461 cccactggaa ctttatttat ttctgtttcc tcgtctatgc ctccctgagg actctgcagg 8521 gggcttttga atgacaccct ggcctgagcc tgggaaactt ggatgctggt cagggctctc 8581 tttcccctgc aaaggaccca gctaagcact tagcagtttg ccatggcctt cccagcatcc 8641 cctgaggcta aagttccacc aagaccgtcg atatcgacga gggacatttc caaacggacc 8701 tccccatctt catttggcct cctgagaagc cacctcagga gctgaggcta agcactaagc 8761 ccaggaccat atgactaggg cactgtacgc cccgccccta gttagagggt aggttttgga 8821 cttggctggg tgtggtcaca gcaatctccc agtgcctttt acagacccag ggctctgccc 8881 tccgccctcg agggccagct tcttgctttc ctagggccct ctcaggatgc ttggctgtgc 8941 tgaggttttt attaaatata tattttatac ttgcggaaag aatgagtgtg tggcagggac 9001 cttgccaggg ctggagacag aggatcccct gcaacagaca ttcccgggct gggggctggc 9061 ggacctgcag gagactttcc gccagccacc ccgtctccag cccctttgga caaatgtcgc 9121 tgtcagtgtt acagatttct tttattgggt tgtttttttg ttgtattttt ttgaacctta 9181 acttattatt ttttttatat ttattgttag aaaatgactt atttctgctc tggaataaag 9241 ttgcagatgg ttcaaaaaaa aaaaggaatt ctgcagatat c //