LOCUS Exported 3361 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-419 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3361) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 3361) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3361 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..42) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 110..214 /gene="bla" /label=AmpR promoter CDS 434..1225 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1396..1984 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1885..1904 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2138..2155 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg 361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac 421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt 481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc 541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt 601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc 661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat 721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga 781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac 841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc 901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc 961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag 1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct 1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga 1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga 1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat 1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact 2221 ctgtggtctc aaatgaacac cggcggtggc caatacacgg attttccggc ggtggaaact 2281 cacggaggac agttcataag ttacgatatc ttcggtagtt tattcgagat cacatctaag 2341 tatcgtcctc cgattatacc aattggtcgt ggagcttatg gaatcgtttg ctctgtgttg 2401 gatacggaga cgaacgagct agtagcgatg aagaagatag ctaatgcttt tgataatcat 2461 atggatgcta agcgtacgct tcgtgagatc aagcttcttc gtcatcttga tcatgaaaac 2521 attatagcta taagagatgt tgttccacca ccactaagaa ggcagttcag tgatgtttat 2581 atctctactg aattaatgga tactgatctt catcaaatca tcagatctaa ccagagttta 2641 tcagaagaac actgtcagta cttcttgtac cagctacttc gaggactgaa gtatatccac 2701 tcagctaaca ttattcatag ggatttaaag ccgagcaatc ttctgttgaa cgcgaattgc 2761 gatttaaaga tttgtgattt cggtcttgct agacctactt cagagaatga ttttatgact 2821 gagtatgttg ttacgagatg gtatagagca cctgagcttc tgttgaactc ttcagattac 2881 acagctgcta ttgatgtttg gtctgttggt tgtatcttta tggagcttat gaatagaaag 2941 cctttgttcc ctggtaaaga ccatgttcat caaatgcgct tattgacaga gttgcttggc 3001 acaccgacag aatctgatct cggttttact cacaatgagg atgcgaaaag atacatccgg 3061 caacttccca acttcccacg tcagccctta gctaaacttt tctctcatgt taacccaatg 3121 gccattgatc ttgttgacag aatgttgacg tttgacccca acagaagaat cactgttgaa 3181 caagctctga atcaccagta ccttgctaaa ttgcacgacc cgaatgatga gccaatctgt 3241 caaaagccat tctcttttga gttcgaacaa cagcctctgg atgaggaaca gataaaagag 3301 atgatctacc aagaagccat agcactcaat ccaacatacg gttaggcttt gagaccacga 3361 a //