LOCUS Exported 5767 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Actin/Focal Adhesions, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-VASP-5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5767) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 5767) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5767 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 553..1230 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 954..973 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1148..1171 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2495..2616 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2532..2551) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2586..2605 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2623..3078) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2760..2781) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2972..2991 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3105..3209 /gene="bla" /label=AmpR promoter promoter 3211..3568 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3234..3254) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3419..3554 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3481..3500 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3603..4397 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3657..3676) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4267..4286 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4585..4604) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4629..4676 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5005..5593 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5494..5513 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtggcta ccatggtgag caagggcgag gaggtcatca aggagttcat gcgcttcaag 601 gagcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 661 cgcccctacg agggcaccca gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc 721 ttcgcctggg acatcctgtc ccctcagatc atgtacggct ccaaggccta cgtgaagcac 781 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 841 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac 901 ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact tcccctccga cggccccgta 961 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 1021 gccctgaagg gcgagatgaa gatgaggctg aggctgaagg acggcggcca ctacgacgcc 1081 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc 1141 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 1201 cgcgccgagg gccgccactc caccggcgcc tccggactca gatctatgag cgagacggtc 1261 atctgttcca gccgggctac tgtgatgctt tatgatgaca gcaacaagcg atggctccct 1321 gctggcactg gtccccaggc cttcagccgc gtccagatat accacaaccc cactgctaac 1381 tccttccgag ttgttggccg caagatgcag ccggatcagc aggtggttat caactgtgcc 1441 atcattcggg gtgtcaagta caatcaggcc actcccatct tccatcagtg gcgagatgcc 1501 cgccaggtct ggggcctcaa cttcggcagc aaggaggacg ccatacagtt tgcaacaggc 1561 atggccaacg ccctagaggc cttggaagga ggtgggcctc ccccagcccc agcaccccct 1621 gcctggtctg cccagaatgg tccctcccca gaggagctgg aacaacagaa aaggcagccg 1681 gagcatatgg agcgccgggt ctccaatgca ggaggcccac ctgctccccc agctgggggc 1741 cctcctccac ctccaggacc tccccctcct ccaggtcccc ccccaccccc aggtctgccc 1801 tcctcagggg tatctggggc aggtcatgga gcaggggcag ccccaccccc tgcaccccca 1861 ctccctacaa cacagggccc caatagtggg ggttccgggg ccccaggcct ggctgctgcc 1921 attgctggag ccaaactcag gaaagtgagc aagcaggagg aggcctctgg ggggcccctg 1981 gcccccaaag ctgagaacag tcgaagcact ggtggggggc ttatggaaga gatgaacgcc 2041 atgctggccc ggagaagaaa agccacacag gttggggaga agccccccaa agacgagtca 2101 gccagtgagg agtcagaggc ccgactccct gcccagagtg aacctgtgag aagaccctgg 2161 gagaagaaca gcacaacctt gccaaggatg aagtcgtcct cttctgtgac tacctccgag 2221 gcccacccct cgacgccctg ctccagtgat gactccgact tggagagggt gaagcaggag 2281 cttctggaag aggtgcggaa ggagctacag aaaatgaaag aggaaatcat cgaagtcttt 2341 gtccaggagc tgaggaagcg gggttctcct tgagcggccg cgactctaga tcataatcag 2401 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 2461 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 2521 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2581 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 2641 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 2701 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 2761 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 2821 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 2881 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 2941 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 3001 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 3061 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 3121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 3181 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 3241 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3301 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 3361 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 3421 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 3481 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 3541 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 3601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 3661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 3721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 3781 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 3841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 3901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 3961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 4021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 4081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 4141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 4201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 4261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 4321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 4381 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 4441 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 4501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 4561 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 4621 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 4681 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 4741 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 4801 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 4861 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4921 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4981 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 5041 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 5101 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 5161 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 5221 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 5281 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 5341 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 5401 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 5461 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 5521 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 5581 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 5641 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 5701 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 5761 agttccg //