LOCUS       Exported                5739 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Localization: Actin, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-Actin-C-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5739)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     954..973
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1148..1171
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2539..2660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2576..2595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2630..2649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2667..3122)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2804..2825)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3016..3035
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3149..3253
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3278..3298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3322..3532
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3344..3540
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3391..3526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3453..3472
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3575..4369
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3629..3648)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4239..4258
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4557..4576)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4601..4648
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4977..5565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5466..5485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtggcta ccatggtgag caagggcgag gaggtcatca aggagttcat gcgcttcaag
      601 gagcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccagg ctgaaggtga ccaagggtgg ccccctgccc
      721 ttcgcctggg acatcctgtc ccctcagatc atgtacggct ccaaggccta cgtgaagcac
      781 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      841 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      901 ggcgagttca tctacaaggt gaaggtgcgc ggcaccaact tcccctccga cggccccgta
      961 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1021 gccctgaagg gcgagatgaa gatgaggctg aggctgaagg acggcggcca ctacgacgcc
     1081 gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc
     1141 gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     1201 cgcgccgagg gccgccactc caccggcgcc tccggactca gatctggcag cggtggaggc
     1261 agcgcatccg gcggaagcgg aagcgatgat gatatcgccg cgctcgtcgt cgacaacggc
     1321 tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt cttcccctcc
     1381 atcgtggggc gccccaggca ccagggcgtg atggtgggca tgggtcagaa ggattcctat
     1441 gtgggcgacg aggcccagag caagagaggc atcctcaccc tgaagtaccc catcgagcac
     1501 ggcatcgtca ccaactggga cgacatggag aaaatctggc accacacctt ctacaatgag
     1561 ctgcgtgtgg ctcccgagga gcaccccgtg ctgctgaccg aggcccccct gaaccccaag
     1621 gccaaccgcg agaagatgac ccagatcatg tttgagacct tcaacacccc agccatgtac
     1681 gttgctatcc aggctgtgct atccctgtac gcctctggcc gtaccactgg catcgtgatg
     1741 gactccggtg acggggtcac ccacactgtg cccatctacg aggggtatgc cctcccccat
     1801 gccatcctgc gtctggacct ggctggccgg gacctgactg actacctcat gaagatcctc
     1861 accgagcgcg gctacagctt caccaccacg gccgagcggg aaatcgtgcg tgacattaag
     1921 gagaagctgt gctacgtcgc cctggacttc gagcaagaga tggccacggc tgcttccagc
     1981 tcctccctgg agaagagcta cgagctgcct gacggccagg tcatcaccat tggcaatgag
     2041 cggttccgct gccctgaggc actcttccag ccttccttcc tgggcatgga gtcctgtggc
     2101 atccacgaaa ctaccttcaa ctccatcatg aagtgtgacg tggacatccg caaagacctg
     2161 tacgccaaca cagtgctgtc tggcggcacc accatgtacc ctggcattgc cgacaggatg
     2221 cagaaggaga tcactgccct ggcacccagc acaatgaaga tcaagatcat tgctcctcct
     2281 gagcgcaagt actccgtgtg gatcggcggc tccatcctgg cctcgctgtc caccttccag
     2341 cagatgtgga tcagcaagca ggagtatgac gagtccggcc cctccatcgt ccaccgcaaa
     2401 tgcttctagg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2461 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     2521 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2641 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     2701 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2761 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2821 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2881 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2941 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3001 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3061 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3121 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3181 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3241 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3361 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     3481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3541 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3601 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3661 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     3721 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     3781 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     3841 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     3901 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     3961 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4021 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4081 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4141 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4201 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4261 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4321 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     4381 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     4441 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     4501 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     4561 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     4621 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     4681 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     4741 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     4801 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     4861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5641 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     5701 tacggggtca ttagttcata gcccatatat ggagttccg
//
