LOCUS Exported 10096 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synOptopatch construct. ACCESSION . VERSION . KEYWORDS LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10096) AUTHORS Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE TITLE All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. JOURNAL Nat Methods. 2018 Oct;15(10):823-831. doi: 10.1038/s41592-018-0142-8. Epub 2018 Oct 1. PUBMED 30275587 REFERENCE 2 (bases 1 to 10096) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10096 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 385..660 /label=chicken beta-actin promoter intron 661..1674 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1598..1620 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1682..1701 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 1729..1740 /label=ZF binding site /bound_moiety="CCR5TC zinc-finger domain" /note="target sequence for the CCR5TC zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 2008; Gross et al., 2013)" CDS 1816..1833 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 1837..1869 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 1873..1896 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" CDS 1924..1980 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKAGHAVRAIGRLSS" primer_bind 2676..2695 /label=mCherry-F /note="mCherry, forward primer" CDS 4858..5199 /codon_start=1 /product="zinc-finger domain from the CCR5-224 (+) zinc-finger nuclease that recognizes the left half-site target sequence in the human CCR5 gene (Perez et al., 2008; Gross et al., 2013)" /label=CCR5TC /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH TKIHLRGSQL" CDS 5215..5271 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5272..6030 /codon_start=1 /product="achaerhodopsin-3 (Arch) derivative with improved voltage sensitivity (Hochbaum et al., 2014)" /label=QuasAr2 /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA TSLRSAARERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD VTAKVGFGFILLRSRAILGDTEAPEPSAGAD" CDS 6100..6810 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGGGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELY" primer_bind complement(6142..6163) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6403..6422) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6750..6771 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(7118..7137) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 7183..7238 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7237..7256) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7599..7615) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7599..7615) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7612..7634) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7623..7639 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7647..7677) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7692..7713 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7772..7967 /label=SV40 promoter /note="SV40 early promoter" rep_origin 7818..7953 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7880..7899 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 7973..8107 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8010..8029) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8064..8083 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8175..8192) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8346..8934) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8426..8445) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9105..9965) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9728..9747 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9966..10070) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg 541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 661 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc 721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 781 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc 841 cttaaagggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg 901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc 961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc 1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc 1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag 1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc 1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc 1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg 1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgt catcctcatc 1741 gccgccacca tgctgcagaa cgagcttgct cttaagttgg ctggacttga tattaacaag 1801 actggaggag gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag 1861 caaatgggtc gggatctgta cgacgatgac gataaggatc tcgcaacaat ggtcgactca 1921 tcacgtcgta agtggaataa ggcaggtcac gcagtcagag ctataggtcg gctgagctca 1981 cccgtggttt ccgagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag 2041 gggctgaggc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc 2101 aagaagcccg tgcagctgcc cggcgcctac atcgtggaca tcaagttgga catcgtgtcc 2161 cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg ccactccacc 2221 ggcggcatgg acgagctgta caagggaggt acaggcggga gtctggtgag caagggcgag 2281 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 2341 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt 2401 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 2461 tcccctcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac 2521 tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac 2581 ggcggcatta ttcacgtgaa ccaggactcc tccctgcagg acggcgtatt catctacaag 2641 gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg 2701 ggctgggagg ctacgcgtga cgacctgact gaagagcaga tcgcagaatt taaagaggct 2761 ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct tgggacggtg 2821 ttccgatcgc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta 2881 gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat ggcaagaaaa 2941 atgaatgaca cagacagtga agaggaaatt cgcgaagcgt tccgcgtgtt tgataaggac 3001 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggagagaag 3061 ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg ggatggtcag 3121 gtaaactacg aagagtttgt acaaatgatg acagcgaagg gtggaggttc tggtggaggt 3181 tctggaggtg gatcaggagg aggttcgaga tctaagctct caggtggcgg agggagcggc 3241 ggaggtggct ctggaggtgg tggctcagct gaagcatggt acaatctggg aaacgcttat 3301 tacaagcagg gcgactatca aaaagccatt gagtattacc aaaaggctct tgagctagac 3361 cctaataacg ccgaagcgtg gtataaccta ggcaacgcat attacaagca gggagactac 3421 caaaaggcaa tcgaatatta ccagaaggca ctcgaactgg acccaaataa cgcagaggct 3481 tggtataatc tgggaaacgc ctactataaa cagggtgact accaaaaggc gatagaggac 3541 tatcagaagg ccttggagct cgatccgaac aatctgcaac gtagtgctgg tggcggaggg 3601 tctggtggag gaggctcagg aggaggtggc gcatcaaacg ttgccaagta cgggcacctt 3661 gaagccagcc ctgatgatgg gcagaatcag ttcagtgata tcattaagtc cagatctgat 3721 gaacacaacg atgtacagaa gaaaaccttt accaaatgga taaacgctcg attttcaaag 3781 agtgggaaac cacccatcaa tgatatgttc tcagacctca aagatgggag aaagctcttg 3841 gatcttctag aaggcctcac aggaacatca ttgccaaagg aacgtggttc cacaagggtg 3901 catgccttaa ataatgtcaa ccgggtgctg caggttttac atcagaacaa tgtggagctg 3961 gtgaatatcg gaggcactga cattgtggat ggaaatccca agctgacgct gggtttgctg 4021 tggagtatta ttctgcactg gcaggtgaag gatgtcatga aagatatcat gtcagacctg 4081 cagcagacga acagcgagaa gatcctgctg agctgggtac ggcagaccac caggccctac 4141 agccaagtca acgtcctcaa cttcaccacc agctggacag atggactcgc attcaacgcc 4201 gtgctccacc ggcacaaacc agatctcttc agctgggaca gagtggtcaa aatgtcccca 4261 actgagagac ttgaacatgc ttttagcaag gcccatactt atttggggat tgaaaaactt 4321 ctggatcctg aagatgttgc cgtgcagctc cctgacaaga aatccataat tatgtattta 4381 acgtctctgt ttgaggtgct tcctcagcaa gtcactatag accaattgaa gctctcaggt 4441 ggcggaggga gcggcggagg tggctctgga ggtggtggct cagctgaagc atggtacaat 4501 ctgggaaacg cttattacaa gcagggcgac tatcaaaaag ccattgagta ttaccaaaag 4561 gctcttgagc tagaccctaa taacgccgaa gcgtggtata acctaggcaa cgcatattac 4621 aagcagggag actaccaaaa ggcaatcgaa tattaccaga aggcactcga actggaccca 4681 aataacgcag aggcttggta taatctggga aacgcctact ataaacaggg tgactaccaa 4741 aaggcgatag aggactatca gaaggccttg gagctcgatc cgaacaatct gcaacgtagt 4801 gctggtggcg gagggtctgg tggaggaggc tcaggaggag gtggcgcatc aaacgaattc 4861 cagtgccgga tctgcatgcg gaacttcagc gaccggtcca acctgagcag gcacatcaga 4921 acccacaccg gagaaaagcc cttcgcctgc gacatttgcg gccggaagtt cgccatcagc 4981 agcaacctga acagccacac caagatccac actggcagcc agaaaccttt ccagtgcaga 5041 atttgtatga gaaactttag cagaagcgac aacctggcca gacacatccg gacacatact 5101 ggtgaaaaac cttttgcctg tgatatctgt ggcagaaagt ttgccacctc cggcaatctg 5161 acccggcaca caaagattca cctgcggggc agccagctat cgattggctc cggagccacg 5221 aacttctctc tgttaaagca agcaggagac gtggaagaaa accccggtcc catggtaagt 5281 atcgctctgc aggctggtta cgacctactg ggtgacggca gacctgaaac cctgtggctg 5341 ggcatcggca ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga 5401 gtcaccgata aggatgcccg ggaatattac gctgtgacta tcctggtgtc cggaatcgca 5461 tccgccgcat atctgtctat gttctttggt atcgggctta ctgaggtgtc cgtcgggggc 5521 gaaatgttgg atatctatta tgccaggtac gcccagtggc tgtttaccac cccacttctg 5581 ctgctgcacc tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt 5641 gtggacgccc tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc 5701 agatacagtt ggtggttgtt ctctacaatt tgcatgatag tggtgctcta tgttctggct 5761 acatccctgc gatctgctgc aagggagcgg ggccccgagg tggcatctac ctttaacacc 5821 ctgacagctc tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact 5881 gagggcgctg gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg 5941 actgccaagg tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc 6001 gaggcaccag aacccagtgc cggtgccgat acgttgatga agagtagaat cacaagcgaa 6061 ggcgagtaca tccccctgga tcaaatagac ataaatgtag tgagcaaggg cgaggagctg 6121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 6181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 6241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggcggcggc 6301 ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 6361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 6421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 6481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 6541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 6601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 6661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg 6721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 6781 gggatcactc tcggcatgga cgagctgtac aaatcaagga tcacctctga gggcgagtat 6841 atccctctgg atcagatcga cattaacgtc ggtggaaaat cccgcataac ctctgagggc 6901 gagtacatcc ctctggatca aatcgatata aacgtggggg gaaaaagtcg aatcacttct 6961 gagggcgagt atatacccct cgatcagatt gacatcaatg tggggggctt ttgttatgag 7021 aatgaagtgt aggcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg 7081 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt 7141 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt 7201 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg 7261 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata 7321 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc 7381 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat 7441 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag 7501 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga 7561 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga 7621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 7681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 7741 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc 7801 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 7861 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 7921 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt 7981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 8041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 8101 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 8161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 8221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 8281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8761 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8881 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8941 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 9001 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 9061 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 9121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 9181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 9241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 9301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 9361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 9421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 10021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 10081 cgaaaagtgc cacctg //