LOCUS Exported 9535 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pWPT-PDX1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9535) AUTHORS Ritz-Laser B, Gauthier BR, Estreicher A, Mamin A, Brun T, Ris F, Salmon P, Halban PA, Trono D, Philippe J TITLE Ectopic expression of the beta-cell specific transcription factor Pdx1 inhibits glucagon gene transcription. JOURNAL Diabetologia. 2003 Jun . 46(6):810-21. PUBMED 12783165 REFERENCE 2 (bases 1 to 9535) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9535 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(861..1072) /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" misc_feature 1129..1246 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(1513..1557) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1742..1975 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 2472..2597 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 2644..3277 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" polyA_signal 3445..3579 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3469..3488) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3523..3542 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(4004..4024) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" intron 4154..4219 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" promoter complement(5353..5682) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5367..5502 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5421..5440) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 5667..5687 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" primer_bind complement(5757..5774) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5928..6516) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6008..6027) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6687..7547) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7310..7329 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7548..7652) /gene="bla" /label=AmpR promoter primer_bind 7720..7738 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(7776..7798) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 7898..7917 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8131..8150 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 8440..8458 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 8440..8457 /label=SP6 /note="SP6 promoter, forward primer" LTR 8483..8663 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" protein_bind complement(8683..8716) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 8860..9448 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 8966..8977 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 9375..9395 /label=WPRE-R /note="WPRE, reverse primer" primer_bind 9461..9477 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 9462..9478 /label=KS primer /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 cgctggaggg ctgtggcgac gcgctaaggc caggcggcag gcggccctct cgggcggcaa 61 cgggggcagc gggcggcaca gcacctccgg ggggcggcgg cggcggcagc gccagaagct 121 cctcgccgga ggtcacggcg cagtcctgct caggctccgc gaccccgcca cccccgacag 181 ctgtcccgcc gccgcgcttc ttgtcctcct cctttttcca cttcatgcgg cggttttgga 241 accagatctt gatgtgtctc tcggtcaagt tcaacatgac agccagctcc acccggcgcg 301 gccgtgagat gtacttgttg aataggaact ccttctccag ctctagcagc tgtgcgcgcg 361 tgtaggccgt gcgcgtccgc ttgttctcct ccggctccgc agcgtaggcg ccgcctgccc 421 actggccttt ccacgcgtga gctttggtag acttcatcca tgggaaaggc agctggacgc 481 ggttgggctc ctccaggacg cccggctcgg ctccctccgg gaagggcccg gcgggcgggt 541 gggggagcgc gagctgagcc gggaggtggt ggtgaaggtg cgccaccgcg gggtcgtcgg 601 cgaggggggg cacctcgtac ggggagatgt ccggggggct gccctgctcc agcgcgccca 661 gggcgccagg gaacgggtgc ggcggcggcg gcgggggctg gcggcccatg tacaggcacg 721 caggggggct ggcgctgaac tccggcgccg ggcctcgctg gaacgcgcat gggtccttgt 781 aaagctgcgt ggccgcgtag tactgctcct cgccgttcat ggccgaattc ctgcagcccg 841 ggggatccgc gtcacgacac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc 901 acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag 961 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg 1021 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg 1081 gcaccggagc catcgatcaa gcttctcgag gctagtctcg tgatcgataa aattttgaat 1141 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat 1201 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg 1261 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc 1321 tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg 1381 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc 1441 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt 1501 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc 1561 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc 1621 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca 1681 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc 1741 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt 1801 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt 1861 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga 1921 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc 1981 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa 2041 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc 2101 tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg 2161 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga 2221 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc 2281 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg 2341 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta 2401 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc 2461 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt 2521 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga 2581 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc 2641 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt 2701 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa 2761 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag 2821 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca 2881 gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct 2941 cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct 3001 aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg 3061 ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc 3121 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct 3181 ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg 3241 atatcttgtc ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat 3301 ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa 3361 atgaaaccac aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt 3421 ttggttagct tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga 3481 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 3541 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 3601 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac 3661 aaatgtggta tggctgatta tgatctctag tcaaggcact atacatcaaa tattccttat 3721 taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag 3781 cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat 3841 gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt 3901 tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac 3961 tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt 4021 ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt 4081 ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc 4141 cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac 4201 ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg 4261 tcacaccaca gaagtaaggt tccttcacaa agatccgggg cccactcata aatccagttg 4321 ccgccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc 4381 atcacaatac gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg ccagatccac 4441 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc 4501 acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta 4561 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga 4621 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc 4681 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca 4741 cggctgttca atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg 4801 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc 4861 aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc 4921 ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca 4981 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtacagaccg 5041 ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt 5101 ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt 5161 gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga 5221 ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca 5281 ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt 5341 gtgtccaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa 5401 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg 5461 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg 5521 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 5581 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc 5641 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctgcattaa 5701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 6361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 7021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 7081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 7141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 7201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 7261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 7321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 7381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 7441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 7501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 7561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 7621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 7681 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 7741 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 7801 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 7861 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 7921 agagtgcacc attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag 7981 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc 8041 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag 8101 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac 8161 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc 8221 gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac 8281 tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt 8341 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg 8401 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat 8461 acacggaatt aattctagat gctgctagag attttccaca ctgactaaaa gggtctgagg 8521 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc 8581 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag 8641 atctggtcta accagagaga cccagtacaa gcaaaaagca gcataacttc gtataatgta 8701 tgctatacga agttattccc atggtgaatt gatcccatct tgtcttcgtt gggagtgaat 8761 tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta 8821 agtcattggt cttaaaggta ccgagctcga attcccgatg cggggaggcg gcccaaaggg 8881 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca 8941 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc 9001 cgcgcagaat ccaggtggca acacaggcga gcagccatgg aaaggacgtc agcttccccg 9061 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc 9121 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga 9181 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca 9241 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt 9301 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta 9361 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt 9421 cacaaatttt gtaatccaga ggttgattat cggaattccc tcgaggtcga cggtatcgat 9481 aagcttgaat ggacagtgtg atcctatcgt ggttcctgcg gccgccgagg cgcga //