LOCUS Exported 10525 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing. ACCESSION . VERSION . KEYWORDS U6-hGRIN2B-CAG-ps-SpCas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10525) AUTHORS Zhou XX, Zou X, Chung HK, Gao Y, Liu Y, Qi LS, Lin MZ TITLE A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for Light-Inducible Gene Editing and Transcription. JOURNAL ACS Chem Biol. 2017 Sep 29. doi: 10.1021/acschembio.7b00603. PUBMED 28938067 REFERENCE 2 (bases 1 to 10525) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10525 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..286 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 288..565 /label=chicken beta-actin promoter intron 566..793 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 805..814 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 814..879 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 886..906 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6427..6447 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6475..6528 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6529..7128 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6529..6548) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind complement(7156..7173) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7162..7369 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 7378..7518 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 7378..7507 /label=AAV2 ITR rep_origin 7593..8048 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7680..7699) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7890..7911 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8065..8084) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8184..8206 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8244..8262) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8330..8434 /gene="bla" /label=AmpR promoter CDS 8435..9295 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8653..8672) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9466..10054 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9955..9974 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 10122..10362 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 10122..10142 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 10293..10312 /label=LKO.1 5' /note="Human U6 promoter, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 121 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 181 cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt 241 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc 301 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 361 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg 421 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa 481 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta 541 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg 601 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt 661 gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt 721 taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat 781 cacttttttt caggttggac cggtgccacc atggactata aggaccacga cggagactac 841 aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg 901 aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc 961 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa 1021 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggcgccctg 1081 ctgttcgaca gcggagaaac agccgaggcc acccggctga agagaaccgc cagaagaaga 1141 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc 1201 aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag 1261 aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag 1321 taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg 1381 cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag 1441 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc 1501 tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc 1561 ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc 1621 gagaagaaga atggcctgtt cggcaacctg attgccctga gcctgggcct gacccccaac 1681 ttcaagagca acttcgacct ggccactagt gtgatcaagc ccgacatgaa aatcaagctg 1741 aggatggagg gtgccgtgaa cggtcacccg tttgcgatcg agggcgtggg actgggcaag 1801 ccgttcgagg gaaagcaaag catcgacctc aaggtgaagg agggcggacc gctgcccttc 1861 gcctacgaca ttctgaccac tgccttctgc tacggtaatc gcgtcttcgc aaagtatccc 1921 gaaaacatcg tggattactt taagcagagc ttcccggagg gttactcctg ggagcgatcc 1981 atgaactacg aggacggagg gatctgcaac gcgacgaacg atataacctt ggacggcgac 2041 tgctacattt acgaaattcg gttccgcggc accaatttcc cagcgaatgg ccctgtaatg 2101 caaaaacgca cggtaaagtg ggagcccagc accgagaacc tgtacgtccg cgacggagtc 2161 ctgaagggag acgtggtcat ggcgctttca ttggaaggtg gcgggcacta cagatgcgat 2221 ttcaagacga cctataaggc gaaaaaggtg gtccaactgc cggactacca tttcgtcgac 2281 caccacatcg agatcaagag ccacgacaaa gactatagca acgtgaatct ccacgagcat 2341 gccgaggccc acagcgagct gcccaggcag gccaagacta gtgaggatgc caaactgcag 2401 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag 2461 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc 2521 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac 2581 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag 2641 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta catcgatggc 2701 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc 2761 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc 2821 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg 2881 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc 2941 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg 3001 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc 3061 gccagcgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag 3121 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc 3181 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa 3241 aaagccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa 3301 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat 3361 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac 3421 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg 3481 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac 3541 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag 3601 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc 3661 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa 3721 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc 3781 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac 3841 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga 3901 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa 3961 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag 4021 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac 4081 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc 4141 tttctgaagg acgactccat cgataacaaa gtgctgactc ggagcgacaa gaaccggggc 4201 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag 4261 ctgctgaatg ccaagctgat tacccagagg aagttcgaca atctgaccaa ggccgagaga 4321 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg 4381 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag 4441 aacgacaaac tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat 4501 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac 4561 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa 4621 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc 4681 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt 4741 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca 4801 aacggcgaaa caggcgagat cgtgtgggat aagggccggg actttgccac cgtgcggaaa 4861 gtgctgtcta tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc 4921 agcaaagagt ctatcctgcc caagaggaac agcgacaagc tgatcgccag aaagaaggac 4981 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg 5041 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg 5101 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag 5161 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag 5221 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa 5281 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg 5341 aagggatccg tgattaaacc agacatgaag atcaagctgc gtatggaagg cgctgtaaat 5401 ggacacccgt tcgcgattga aggagttggc cttgggaagc ctttcgaggg aaaacagagt 5461 attgacctta aagtcaaaga aggcggacct ctgcctttcg cctatgacat cttgacaact 5521 gctttctgtt acggcaacag ggtattcgcc aaatacccag aaaatatagt agactatttc 5581 aagcagtcgt ttcctgaggg ctactcttgg gaacgaagca tgaattacga agacgggggc 5641 atttgtaacg cgacaaacga cataaccctg gatggtgact gttatatcta tgaaattcga 5701 tttagaggta cgaactttcc tgccaatggt ccagttatgc agaagaggac tgtgaaatgg 5761 gagccatcca ctgagaactt gtatgtgcgt gatggagtgc tgaagggtga tgttgtcatg 5821 gctctgtcgc ttgaaggagg tggccattac cgatgtgact tcaaaactac ttataaagct 5881 aagaaggttg tccagttgcc agactatcac tttgtggacc accacattga gattaaaagc 5941 cacgacaaag attacagtaa tgttaatctg catgagcacg ccgaagcgca ttctgagctg 6001 ccgaggcagg ccaagggatc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 6061 cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 6121 ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct 6181 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 6241 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag 6301 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 6361 ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaagctagc 6421 ggctccccca agaaaaaacg caaggtgggc ttaattaacg gaagcggcag tggagagggc 6481 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat gaccgagtac 6541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 6601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 7021 ccccacaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 7081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctaacta 7141 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 7201 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 7261 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 7321 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag cggccgcagg 7381 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 7441 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 7501 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 7561 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc 7621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc 7681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 7741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 7801 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 7861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 7921 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg 7981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 8041 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 8101 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 8161 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 8221 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 8281 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 8341 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 8401 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 8461 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 8521 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 8581 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 8641 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 8701 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 8761 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 8821 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 8881 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 8941 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 9001 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 9061 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 9121 tgctgataaa tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc 9181 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 9241 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 9301 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 9361 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 9421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 9481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 9541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 9601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 9661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 9721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 9781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 9841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 9901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 9961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 10021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 10081 gttcctggcc ttttgctggc cttttgctca catgtttcga agagggccta tttcccatga 10141 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga 10201 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt 10261 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa 10321 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gggagaacag 10381 cactccgctc tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt 10441 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgtttta gagctagaaa 10501 tagcaagtta aaatctagag gtacc //