LOCUS Exported 10826 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone. ACCESSION . VERSION . KEYWORDS pFUGW-hGtACR2-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10826) AUTHORS Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL TITLE Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. JOURNAL Science. 2015 Jun 25. pii: aaa7484. PUBMED 26113638 REFERENCE 2 (bases 1 to 10826) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10826 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 26..47 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(166..186) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 171..500 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 351..486 /label=SV40 ori /note="SV40 origin of replication" primer_bind 413..432 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 548..595 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 614..988 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 1118..1239 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1155..1174) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1209..1228 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1288..1304) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1288..1304) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1301..1323) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1312..1328 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1336..1366) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1381..1402 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1519..1536) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1690..2278) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1770..1789) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2449..3309) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3072..3091 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3310..3414) /gene="bla" /label=AmpR promoter primer_bind complement(3489..3508) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 3680..4059 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4061..4259 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4214..4234 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 4277..4457 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 4504..4629 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 5122..5355 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 5540..5584 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 5882..5999 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 6091..7302 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 7139..7158 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 8219..8935 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(8261..8282) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(8522..8541) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 8869..8890 /label=EGFP-C /note="EGFP, forward primer" misc_feature 8960..9548 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9013..9033) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9431..9442) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(9551..9567) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9552..9568) /label=pBluescriptKS /note="For pBluescript vector" LTR 10073..10253 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(10279..10296) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 10285..10509 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 10555..157 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10642..10661) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 61 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt 601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga 721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca 781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc 841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga 901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt 961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc 1021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 1081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 1141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 1201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 1261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 1321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 1381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 1441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 1501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 1561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 1621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 1681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 1741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 1801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 1861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 2041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 2101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 2161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 2221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 2281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 2461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 2521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 2581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 2641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 2701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 2761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 2821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 2881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 2941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 3001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 3061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 3121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 3181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 3241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 3301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 3361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 3421 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc 3481 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt 3541 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg 3601 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta 3661 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg 3721 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc 3781 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 3841 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 3901 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 3961 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 4021 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac 4081 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac 4141 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac 4201 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc 4261 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 4321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 4381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 4441 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag 4501 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg 4561 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc 4621 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg 4681 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa 4741 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga 4801 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta 4861 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac 4921 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt 4981 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt 5041 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag 5101 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag 5161 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat 5221 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact 5281 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa 5341 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt 5401 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg 5461 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga 5521 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag 5581 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat 5641 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt 5701 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga 5761 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt 5821 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca 5881 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa 5941 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc 6001 gggtttatta cagggacagc agagatccag tttggttaat cgcatgctag ctagttcgaa 6061 ccttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg 6121 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc agcgagcgtc 6181 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag 6241 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg 6301 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg 6361 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac 6421 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc 6481 gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct 6541 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag 6601 gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg 6661 aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg 6721 ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg 6781 agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact 6841 cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt 6901 acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat 6961 gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag 7021 ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag 7081 ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg 7141 aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag 7201 gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa 7261 ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg ggctgcaggt 7321 cgactctaga ggatccatgg cctcccaggt cgtgtacggc gagtgggcca gcacccacac 7381 agagtgctac aacatgagca ggatcgacag caccttcgtg agcctgctgc agctggtgtg 7441 ggccgtggtg agcggatgcc agaccatctt catgatcagc agggccccca aggtgccctg 7501 ggagagcgtg tacctgccct tcgtggagtc catcacatac gccctggcca gcaccggcaa 7561 tggcacactg cagatgaggg acggcaggtt ctttccctgg tccaggatgg ccagctggct 7621 gtgcacatgc cccattatgc tgggccagat ctccaacatg gctctggtga agtacaagag 7681 catccctctg aaccccatcg cccaggccgc cagcattatc agggtggtga tgggcattac 7741 cgctaccatc tcccccgccg agtacatgaa gtggctgttc ttcttcttcg gcgctacctg 7801 cctggtcttc gaatacagcg tggtgttcac catcttccag gtgggactgt acggcttcga 7861 gtccgtgggc acccctctgg cccagaaggt ggtggtgagg atcaagatgc tgaggctgat 7921 cttcttcatc gcctggacca tgttccccat cgtgtggctg atttccccca ccggcgtgtg 7981 cgtgatccac gagaacgtga gcgccatcct gtatctgctc gccgacggcc tgtgcaagaa 8041 cacctacggc gtgatcctgt ggtccaccgc ctggggagtg ctcgagggaa agtgggaccc 8101 ggcttgcctc cctggccagg agaagcctga ggccgacgac cccttcggac tgaaccacga 8161 gaagaatgcc ccccccaacg acgaggtcaa catcagaatg ttcggcaggg cggccgccgt 8221 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 8281 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 8341 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 8401 gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca 8461 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 8521 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 8581 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 8641 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 8701 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 8761 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 8821 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 8881 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt 8941 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 9001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 9061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 9121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 9181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 9241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 9301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 9361 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 9421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 9481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 9541 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag 9601 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 9661 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 9721 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 9781 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa 9841 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt 9901 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca 9961 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga 10021 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct 10081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 10141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 10201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg 10261 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 10321 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 10381 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 10441 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 10501 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 10561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 10621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 10681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 10741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 10801 cctgatagac ggtttttcgc cctttg //