LOCUS Exported 5507 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION E.coli/H.Pylori shuttle vector. ACCESSION . VERSION . KEYWORDS pHel3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5507) AUTHORS Heuermann D, Haas R TITLE A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation. JOURNAL Mol Gen Genet. 1998 Mar;257(5):519-28. PUBMED 9563837 REFERENCE 2 (bases 1 to 5507) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5507 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(2304..3098) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" rep_origin complement(3868..4456) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3948..3967) /label=pBR322ori-F /note="pBR322 origin, forward primer" oriT 4682..4791 /note="incP origin of transfer" ORIGIN 1 catttttgat agtagtgatt ttagtggtaa tcttgacaca gccactatat taaaacctta 61 gcgttttaat aacccttata agtccgccaa gacttcttaa gggtttcact cctgttatta 121 tatcgtcttt tgaaaaataa gcattaaaag gcgcttaaat gcccatgaat acgaattttg 181 aacagcttag aaaacaagaa ttggaattac gaaaattatt agaagaatta gaaacgctcc 241 cacaaacccc acaaattaaa ctgcaaaaac aaaaaataca aacttacata gacaagataa 301 caccaagtat tttgagcggt tttgatcaaa aattcaaaga aattatagaa aatctatcaa 361 atgaatttga aaaagaaaaa tccacaccac tcaaagagcc acaaaccacc cccacaccat 421 gcaaagattt agtggttagc acccctaaag ataacaccta taccacctac cacaataacg 481 ctaataaggt caatctaggg aaattgagcg aaagggaagc caatctttta ttcgctattt 541 ttcaaaaact caaagatcaa gggaataccc tcattcgttt tgaaccgcaa gatttgaaac 601 gcatgatcat agtcaaatcc aacctaacca acagacaatt attgcaaatc ctcaaaaact 661 tacttgacaa cataggcggt gctaattttt ggatcattag agagcatgtt gaaaatggcg 721 aaatctatga agatcacact agctacatgc ttttcaaaca atttgacatt cgtatccata 781 agccaaccca aactatagaa tacttagaag tccaactcaa cgatagctac cactacctgc 841 tcaacaatct aggcatgagc ggtcaataca cttcctttaa acttttagaa ttccaaaggg 901 tgaggggcaa atacgccaag acgctctatc gcttactcaa gcaatacaaa agcacaggga 961 ttttgagcgt ggaatgggat caattcaggg agcttttaga cattccaaaa gattacaaaa 1021 tggaaaacat cgatcaaaaa gtcttaactc caagcctcaa ggaactcaga aaaatctatc 1081 cctttgaaca cttgagctac aaaaaagaac gcaaaagcca cgacaaacgc aaggtaacgc 1141 atattgattt ttattttgag caattgccac agggcgaaac caagaaacaa aaacaagccg 1201 acaagcaacg cgctcaaagg gacatcaagc tcatagcgtg ggatattcac aaccaaatcg 1261 ctaaaagaaa cgctaaagcc actatagaag ctaggtttct tgagctaaaa accttgattg 1321 gttatcagtt caggcacaac aatgggacta ttttacagat tgacaacgcc acttttgaaa 1381 agaatcaaat gtttttgcat gtttcaacca gcaaaaattc tcaaaaattc cttgtgtcca 1441 acaaaacatt cgctttagaa cttctgtttg tcaatggata cagcctaaaa aaagacatgt 1501 tggaaattga tcccccaagt atccacccca tcaccaacga acctatcaag gaatttgcag 1561 aatacatcgg aaaaacgatc cacatcacca atttcaatgt ggatcaatgc cctgagggga 1621 tcaataacta cctgaaaatc actaggattg tgaaactgaa tgacaatcgg atctgtgttt 1681 cagtccaaga tgtggataag cctgagaaac ttctaaaacc tttcattgct aaagatgaaa 1741 aacatttgaa aaattggttt aagaaacact atcggtgaaa aataagtggt gggattaaca 1801 atcgtccccc acaccttgca atgtagttag ttttgcatta ttttttctat aatctctatc 1861 aattctgaga gaacatagat taattcaatg aaatttttca ttatctattt cctttctgta 1921 aattttgtta aatatttatt aaaaaaaaat ttaaagatca ctctttaagt ggaacataca 1981 aaggctgtag agagtgccac attaagcctg atgttttgct tgtgtataga gtgaaaggca 2041 atattttaac tttagttagg cttggcagtc atagtgaatt gttttagaca aatttctttc 2101 taaaatcgcc cagaatgaat ttttaattct aatttaatat aattcatcgg ttttcactca 2161 aaacgatgaa atagggctat ttttagctca ttattggggt attttgtttt tagttgaaag 2221 ctaacttcaa aattcaaaaa aacttcattt ccctttctag aggatcgatc cccggggatc 2281 ttttagacat ctaaatctag gtactaaaac aattcatcca gtaaaatata atattttatt 2341 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc 2401 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg 2461 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg 2521 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa 2581 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca 2641 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc 2701 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc 2761 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg 2821 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga 2881 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg 2941 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccaac 3001 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc 3061 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc 3121 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt 3181 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt 3241 tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatga tagaatttac 3301 aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg 3361 cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac 3421 atacggcgat atcttctata taagcgtacc ggttccaatt ttttcgcagt ttaacttttc 3481 cgacgcattt atcgtcttgg tagcttttta aatatggcgc ttcatagagt aattctgtaa 3541 aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca aatggttcgc 3601 tgggtttgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc aggcatgcaa 3661 gctagctttc gcgagctcga gatctagata tcgatgaatt caaaaaaaaa ggctccaaaa 3721 ggagccttta atgatctaat tcgtatcagc tcactcaaag gcggtaatac ggttatccac 3781 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3841 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3901 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3961 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 4021 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 4081 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 4141 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 4201 cttatcgcca ccggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4261 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4321 tatctgcgct ctgctgaaac cagttacctt cggaaaaaga gttggtagct cttgatccgg 4381 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4441 aaaaaaagga tctcaagaag atcctttgat ctctgcagtt cagtaatttc ctgcatttgc 4501 ctgtttccag tcggtagata ttccacaaaa cagcagggaa gcagcgcttt tccgctgcat 4561 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat 4621 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc 4681 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt 4741 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg 4801 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gctgcagaag cttgtccgtt 4861 agcaaatata atgtgtttag cacctaaaag cgcatctatc ttgtgttagc catctaacct 4921 aaacaaaacc gcctagcgag cgttagcgag catggacaaa agcgcatcgc agtttgaaat 4981 cgtaggcgtt agccggagat ggttttgcgt tagcaaaatc aaacaagata gcgcaaaccg 5041 aggcgtcagc caaaaaaacc ctaaaactaa aatccccaaa atatgtagcg cgtcatgcgc 5101 gttgtttata ttacatgttt taataactat gttgttttta catgttttta caacgcgcgc 5161 gcgtgtgagg gattgggggt tgcaaccccc taaataacga agctgtaggg tttctcattt 5221 ttgggttaaa atgaataaaa cagaacttct tacgagtgat aacagaactt cttacgagtg 5281 ataacagaac ttcttacgag tgataacaga acttcttacg agtgataaca gaacttcttt 5341 attgtgaagt tatgattatt aacaattttt agctaaaata acaacctaag gcggtgtaac 5401 atggttgcta atcctagtgt taacaaattt ggagcaatta gctttaaaag ctagtgggtt 5461 gggagtttgt agcgggtagc actccgttag gaggcacacc atgaaag //