LOCUS Exported 5116 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION E.coli/H.Pylori shuttle vector. ACCESSION . VERSION . KEYWORDS pHel2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5116) AUTHORS Heuermann D, Haas R TITLE A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation. JOURNAL Mol Gen Genet. 1998 Mar;257(5):519-28. PUBMED 9563837 REFERENCE 2 (bases 1 to 5116) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5116 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2751..3410 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2817..2836) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 3539..3587 /label=fd terminator /note="central terminator from bacteriophage fd (Otsuka and Kunisawa, 1982)" rep_origin complement(3860..4448) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3940..3959) /label=pBR322ori-F /note="pBR322 origin, forward primer" oriT 4674..4783 /note="incP origin of transfer" ORIGIN 1 aactatgttg tttttacatg tttttacaac gcgcgcgcgt gtgagggatt gggggttgca 61 accccctaaa taacgaagct gtagggtttc tcatttttgg gttaaaatga ataaaacaga 121 acttcttacg agtgataaca gaacttctta cgagtgataa cagaacttct tacgagtgat 181 aacagaactt cttacgagtg ataacagaac ttctttattg tgaagttatg attattaaca 241 atttttagct aaaataacaa cctaaggcgg tgtaacatgg ttgctaatcc tagtgttaac 301 aaatttggag caattagctt taaaagctag tgggttggga gtttgtagcg ggtagcactc 361 cgttaggagg cacaccatga aagcattttt gatagtagtg attttagtgg taatcttgac 421 acagccacta tattaaaacc ttagcgtttt aataaccctt ataagtccgc caagacttct 481 taagggtttc actcctgtta ttatatcgtc ttttgaaaaa taagcattaa aaggcgctta 541 aatgcccatg aatacgaatt ttgaacagct tagaaaacaa gaattggaat tacgaaaatt 601 attagaagaa ttagaaacgc tcccacaaac cccacaaatt aaactgcaaa aacaaaaaat 661 acaaacttac atagacaaga taacaccaag tattttgagc ggttttgatc aaaaattcaa 721 agaaattata gaaaatctat caaatgaatt tgaaaaagaa aaatccacac cactcaaaga 781 gccacaaacc acccccacac catgcaaaga tttagtggtt agcaccccta aagataacac 841 ctataccacc taccacaata acgctaataa ggtcaatcta gggaaattga gcgaaaggga 901 agccaatctt ttattcgcta tttttcaaaa actcaaagat caagggaata ccctcattcg 961 ttttgaaccg caagatttga aacgcatgat catagtcaaa tccaacctaa ccaacagaca 1021 attattgcaa atcctcaaaa acttacttga caacataggc ggtgctaatt tttggatcat 1081 tagagagcat gttgaaaatg gcgaaatcta tgaagatcac actagctaca tgcttttcaa 1141 acaatttgac attcgtatcc ataagccaac ccaaactata gaatacttag aagtccaact 1201 caacgatagc taccactacc tgctcaacaa tctaggcatg agcggtcaat acacttcctt 1261 taaactttta gaattccaaa gggtgagggg caaatacgcc aagacgctct atcgcttact 1321 caagcaatac aaaagcacag ggattttgag cgtggaatgg gatcaattca gggagctttt 1381 agacattcca aaagattaca aaatggaaaa catcgatcaa aaagtcttaa ctccaagcct 1441 caaggaactc agaaaaatct atccctttga acacttgagc tacaaaaaag aacgcaaaag 1501 ccacgacaaa cgcaaggtaa cgcatattga tttttatttt gagcaattgc cacagggcga 1561 aaccaagaaa caaaaacaag ccgacaagca acgcgctcaa agggacatca agctcatagc 1621 gtgggatatt cacaaccaaa tcgctaaaag aaacgctaaa gccactatag aagctaggtt 1681 tcttgagcta aaaaccttga ttggttatca gttcaggcac aacaatggga ctattttaca 1741 gattgacaac gccacttttg aaaagaatca aatgtttttg catgtttcaa ccagcaaaaa 1801 ttctcaaaaa ttccttgtgt ccaacaaaac attcgcttta gaacttctgt ttgtcaatgg 1861 atacagccta aaaaaagaca tgttggaaat tgatccccca agtatccacc ccatcaccaa 1921 cgaacctatc aaggaatttg cagaatacat cggaaaaacg atccacatca ccaatttcaa 1981 tgtggatcaa tgccctgagg ggatcaataa ctacctgaaa atcactagga ttgtgaaact 2041 gaatgacaat cggatctgtg tttcagtcca agatgtggat aagcctgaga aacttctaaa 2101 acctttcatt gctaaagatg aaaaacattt gaaaaattgg tttaagaaac actatcggtg 2161 aaaaataagt ggtgggatta acaatcgtcc cccacacctt gcaatgtagt tagttttgca 2221 ttattttttc tataatctct atcaattctg agagaacata gattaattca atgaaatttt 2281 tcattatcta tttcctttct gtaaattttg ttaaatattt attaaaaaaa aatttaaaga 2341 tcactcttta agtggaacat acaaaggctg tagagagtgc cacattaagc ctgatgtttt 2401 gcttgtgtat agagtgaaag gcaatatttt aactttagtt aggcttggca gtcatagtga 2461 attgttttag acaaatttct ttctaaaatc gcccagaatg aatttttaat tctaatttaa 2521 tataattcat cggttttcac tcaaaacgat gaaatagggc tatttttagc tcattattgg 2581 ggtattttgt ttttagttga aagctaactt caaaattcaa aaaaacttca tttccctttc 2641 tagagatccg gtttttgtta atccgccata ttgtgttgaa acaccgcccg gaacccgata 2701 taatccgccc ttcaacagat ccgagatttt caggagctaa ggaagctaaa atggagaaaa 2761 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 2821 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 2881 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 2941 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 3001 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 3061 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 3121 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 3181 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 3241 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 3301 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 3361 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 3421 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga 3481 tgaatggcag aaattcggat cttccatacc taccagttct gcgcctgcag gtcgataaac 3541 cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa cgtggatctg 3601 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagctagctt 3661 tcgcgagctc gagatctaga tatcgatgaa ttcaaaaaaa aaggctccaa aaggagcctt 3721 taatgatcta attcgtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3781 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3841 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3901 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3961 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4021 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4081 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 4141 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 4201 caccggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4261 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 4321 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4381 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4441 gatctcaaga agatcctttg atctctgcag ttcagtaatt tcctgcattt gcctgtttcc 4501 agtcggtaga tattccacaa aacagcaggg aagcagcgct tttccgctgc ataaccctgc 4561 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga 4621 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg 4681 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac 4741 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca 4801 gatgagggca agcggatggc tgatgaaacc aagctgcaga agcttgtccg ttagcaaata 4861 taatgtgttt agcacctaaa agcgcatcta tcttgtgtta gccatctaac ctaaacaaaa 4921 ccgcctagcg agcgttagcg agcatggaca aaagcgcatc gcagtttgaa atcgtaggcg 4981 ttagccggag atggttttgc gttagcaaaa tcaaacaaga tagcgcaaac cgaggcgtca 5041 gccaaaaaaa ccctaaaact aaaatcccca aaatatgtag cgcgtcatgc gcgttgttta 5101 tattacatgt tttaat //