LOCUS Exported 3896 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION gene cloning of human CD1A. ACCESSION . VERSION . KEYWORDS pHCD1A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3896) AUTHORS Manolova V, Hirabayashi Y, Mori L, De Libero G TITLE CD1a and CD1b surface expression is independent from de novo synthesized glycosphingolipids. JOURNAL Eur J Immunol. 2003 Jan;33(1):29-37. PUBMED 12594829 REFERENCE 2 (bases 1 to 3896) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3896 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..19 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1037..1053) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1037..1053) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(1085..1104) /label=T7 /note="T7 promoter, forward primer" promoter complement(1086..1104) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1111..1128) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1111..1127) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1120..1142) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 1269..1724 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1356..1375) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1566..1587 /label=F1ori-F /note="F1 origin, forward primer" promoter 1750..1854 /gene="bla" /label=AmpR promoter CDS 1855..2715 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2073..2092) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2886..3474 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3375..3394 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3628..3645 /label=L4440 /note="L4440 vector, forward primer" protein_bind 3762..3783 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3798..3828 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3836..3852 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3841..3863 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3860..3876 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3860..3876 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga gatgctgttt 61 ttgctacttc cattgttagc tgttctccca ggtgatggca atgcagacgg gctcaaggag 121 cctctctcct tccatgtcat ctggatcgca tccttttaca accattcctg gaaacaaaat 181 ctggtctcag gttggctgag tgatttgcag actcatacct gggacagcaa ttccagcacc 241 atcgttttcc tgtggccctg gtccagggga aacttcagca atgaggagtg gaaggaactg 301 gaaacattat tccgtatacg caccattcgg tcatttgagg gaattcgtag atacgcccat 361 gaattgcagt ttgaatatcc ttttgagata caggtgacag gaggctgtga gctgcactct 421 ggaaaggtct caggaagctt cttgcagtta gcttatcaag gatcagactt tgtgagcttc 481 cagaacaatt catggttgcc atatccagtg gctgggaata tggccaagca tttctgcaaa 541 gtgctcaatc agaatcagca tgaaaatgac ataacacaca atcttctcag tgacacctgc 601 ccacgtttca tcttgggtct tcttgatgca ggaaaggcac atctccagcg gcaagtgaag 661 cccgaggcct ggctgtccca tggccccagt cctggccctg gccatctgca gcttgtgtgc 721 catgtctcag gattctaccc aaagcccgtg tgggtgatgt ggatgcgggg tgagcaggag 781 cagcagggca ctcagcgagg ggacatcttg cccagtgctg atgggacatg gtatctccgc 841 gcaaccctgg aggtggccgc tggggaggca gctgacctgt cctgtcgggt gaagcacagc 901 agtctagagg gccaggacat cgtcctctac tgggagcatc acagttccgt gggcttcatc 961 atcttggcgg tgatagtgcc tttacttctt ctgataggtc ttgcgctttg gttcaggaaa 1021 cgctgtttct gttaaggatc cactagttct agagcggccg ccaccgcggt ggagctccaa 1081 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg 1141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 1201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 1261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 1321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 1381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 1441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 1501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 1561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 1621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 1681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 1741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 1861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 2041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 2101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 2161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 2221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 2281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 2821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 3001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 3661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 3721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 3781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 3841 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcga //